Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno
Dione juno and Agraulis vanillae are very common butterflies in natural gardens in South America, and also bred worldwide. In addition, larvae of these butterflies are considered as pests in crops of Passiflora spp. For these reasons, it is important to identify and describe pathogens of these speci...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/21110 |
| _version_ | 1855038348497780736 |
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| author | Rodríguez, Vanina Andrea Belaich, Mariano Nicolás Mengual Gómez, Diego Luis Sciocco, Alicia Ines Ghiringhelli, Pablo Daniel |
| author_browse | Belaich, Mariano Nicolás Ghiringhelli, Pablo Daniel Mengual Gómez, Diego Luis Rodríguez, Vanina Andrea Sciocco, Alicia Ines |
| author_facet | Rodríguez, Vanina Andrea Belaich, Mariano Nicolás Mengual Gómez, Diego Luis Sciocco, Alicia Ines Ghiringhelli, Pablo Daniel |
| author_sort | Rodríguez, Vanina Andrea |
| collection | INTA Digital |
| description | Dione juno and Agraulis vanillae are very common butterflies in natural gardens in South America, and also bred worldwide. In addition, larvae of these butterflies are considered as pests in crops of Passiflora spp. For these reasons, it is important to identify and describe pathogens of these species, both for preservation purposes and for use in pest control. Baculoviridae is a family of insect viruses that predominantly infect species of Lepidoptera and are used as bioinsecticides. Larvae of D. juno and A. vanillae exhibiting symptoms of baculovirus infection were examined for the presence of baculoviruses by PCR and transmission electron microscopy. Degenerate primers were designed and used to amplify partial sequences from the baculovirus p74, cathepsin, and chitinase genes, along with previously designed primers for amplification of lef-8, lef-9, and polh. Sequence data from these six loci, along with ultrastructural observations on occlusion bodies isolated from the larvae, confirmed that the larvae were infected with nucleopolyhedroviruses from genus Alphabaculovirus. The NPVs from the two different larval hosts appear to be variants of the same, previously undescribed baculovirus species. Phylogenetic analysis of the sequence data placed these NPVs in Alphabaculovirus group I/clade 1b. |
| format | info:ar-repo/semantics/artículo |
| id | INTA21110 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA211102025-01-31T14:24:31Z Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno Rodríguez, Vanina Andrea Belaich, Mariano Nicolás Mengual Gómez, Diego Luis Sciocco, Alicia Ines Ghiringhelli, Pablo Daniel Control biológico de plagas Biological pest control Lepidoptera Virus Viruses Nymphalidae Dione juno Agraulis vanillae Butterflies Nucleopolyhedrovirus Dione juno and Agraulis vanillae are very common butterflies in natural gardens in South America, and also bred worldwide. In addition, larvae of these butterflies are considered as pests in crops of Passiflora spp. For these reasons, it is important to identify and describe pathogens of these species, both for preservation purposes and for use in pest control. Baculoviridae is a family of insect viruses that predominantly infect species of Lepidoptera and are used as bioinsecticides. Larvae of D. juno and A. vanillae exhibiting symptoms of baculovirus infection were examined for the presence of baculoviruses by PCR and transmission electron microscopy. Degenerate primers were designed and used to amplify partial sequences from the baculovirus p74, cathepsin, and chitinase genes, along with previously designed primers for amplification of lef-8, lef-9, and polh. Sequence data from these six loci, along with ultrastructural observations on occlusion bodies isolated from the larvae, confirmed that the larvae were infected with nucleopolyhedroviruses from genus Alphabaculovirus. The NPVs from the two different larval hosts appear to be variants of the same, previously undescribed baculovirus species. Phylogenetic analysis of the sequence data placed these NPVs in Alphabaculovirus group I/clade 1b. Instituto de Microbiología y Zoología Agrícola (IMYZA) Fil: Rodríguez, Vanina Andrea. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; Argentina Fil: Rodríguez, Vanina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Belaich, Mariano Nicolás. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; Argentina Fil: Belaich, Mariano Nicolás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Mengual Gómez, Diego Luis. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; Argentina Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola (IMyZA); Argentina Fil: Ghiringhelli, Pablo Daniel. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Ingeniería Genética y Biología Celular y Molecular; Argentina 2025-01-31T13:50:53Z 2025-01-31T13:50:53Z 2011-02-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/21110 Journal of Invertebrate Pathology 106 (2) : 255-262 (Febrero 2011) 0022-2011 1096-0805 (on line) eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Journal of Invertebrate Pathology 106 (2) : 255-262. (Febrero 2011) |
| spellingShingle | Control biológico de plagas Biological pest control Lepidoptera Virus Viruses Nymphalidae Dione juno Agraulis vanillae Butterflies Nucleopolyhedrovirus Rodríguez, Vanina Andrea Belaich, Mariano Nicolás Mengual Gómez, Diego Luis Sciocco, Alicia Ines Ghiringhelli, Pablo Daniel Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno |
| title | Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno |
| title_full | Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno |
| title_fullStr | Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno |
| title_full_unstemmed | Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno |
| title_short | Identification of nucleopolyhedrovirus that infect Nymphalid butterflies Agraulis vanillae and Dione juno |
| title_sort | identification of nucleopolyhedrovirus that infect nymphalid butterflies agraulis vanillae and dione juno |
| topic | Control biológico de plagas Biological pest control Lepidoptera Virus Viruses Nymphalidae Dione juno Agraulis vanillae Butterflies Nucleopolyhedrovirus |
| url | http://hdl.handle.net/20.500.12123/21110 |
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