Screening of bioactive compounds in lettuce: multivariate optimization of an ultrasound‑assisted solid–liquid extraction procedure
Lettuce (Lactuca sativa L.) is one of the most consumed vegetables worldwide and is considered a good source of healthpromoting compounds. The objective of this work was to apply a multivariate design of experiments through a screening stage followed by optimization of factors that afect the extract...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Springer
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/20392 https://link.springer.com/article/10.1007/s12161-024-02656-z https://doi.org/10.1007/s12161-024-02656-z |
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| author | Lemos, Aldana Ayelén Soto Vargas, Verónica Carolina Wuilloud, Rodolfo Germán Gonzalez, Roxana Elizabeth |
| author_browse | Gonzalez, Roxana Elizabeth Lemos, Aldana Ayelén Soto Vargas, Verónica Carolina Wuilloud, Rodolfo Germán |
| author_facet | Lemos, Aldana Ayelén Soto Vargas, Verónica Carolina Wuilloud, Rodolfo Germán Gonzalez, Roxana Elizabeth |
| author_sort | Lemos, Aldana Ayelén |
| collection | INTA Digital |
| description | Lettuce (Lactuca sativa L.) is one of the most consumed vegetables worldwide and is considered a good source of healthpromoting compounds. The objective of this work was to apply a multivariate design of experiments through a screening stage followed by optimization of factors that afect the extraction of phytochemicals present in lettuce. A response surface methodology was employed and, specifcally, a central composite design model, for the optimization of the methods. The optimal conditions for the extraction of chlorophyll a, b, and total carotenoids were as follows: 4.8 mL of acetone:water (80:20 v/v) and 10 mg of sample with ultrasound-assisted extraction for 5 min. On the other hand, for anthocyanin and phenolic compounds, the optimal conditions were as follows: 5 mL of acetone:acidifed water (80:20 v/v) and 20 mg of sample for 5 min with ultrasound-assisted extraction. The experimental model was followed by spectrophotometry methodology for a comprehensive characterization. Optimal conditions were applied for the simultaneous extraction and determination of the above-mentioned compounds in diferent lettuce cultivars. Both extraction methods successfully extract diferent phytochemicals by reducing the volume of solvent, sample mass, and extraction time compared to previously reported methods. The results obtained showed a signifcant variability in the content of phytochemicals; therefore, it could be estimated that the diferent cultivars of lettuce evaluated will exhibit diferent benefcial health efects. |
| format | info:ar-repo/semantics/artículo |
| id | INTA20392 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA203922025-08-19T11:33:47Z Screening of bioactive compounds in lettuce: multivariate optimization of an ultrasound‑assisted solid–liquid extraction procedure Lemos, Aldana Ayelén Soto Vargas, Verónica Carolina Wuilloud, Rodolfo Germán Gonzalez, Roxana Elizabeth Compuestos Bioactivos Lechugas Lactuca sativa Fitoquímicos Diseño Experimental Bioactive Compounds Lettuces Phytochemicals Experimental Design Lactuca sativa L. Quantification Lettuce (Lactuca sativa L.) is one of the most consumed vegetables worldwide and is considered a good source of healthpromoting compounds. The objective of this work was to apply a multivariate design of experiments through a screening stage followed by optimization of factors that afect the extraction of phytochemicals present in lettuce. A response surface methodology was employed and, specifcally, a central composite design model, for the optimization of the methods. The optimal conditions for the extraction of chlorophyll a, b, and total carotenoids were as follows: 4.8 mL of acetone:water (80:20 v/v) and 10 mg of sample with ultrasound-assisted extraction for 5 min. On the other hand, for anthocyanin and phenolic compounds, the optimal conditions were as follows: 5 mL of acetone:acidifed water (80:20 v/v) and 20 mg of sample for 5 min with ultrasound-assisted extraction. The experimental model was followed by spectrophotometry methodology for a comprehensive characterization. Optimal conditions were applied for the simultaneous extraction and determination of the above-mentioned compounds in diferent lettuce cultivars. Both extraction methods successfully extract diferent phytochemicals by reducing the volume of solvent, sample mass, and extraction time compared to previously reported methods. The results obtained showed a signifcant variability in the content of phytochemicals; therefore, it could be estimated that the diferent cultivars of lettuce evaluated will exhibit diferent benefcial health efects. EEA Mendoza Fil: Lemos, Aldana. Universidad Nacional de Cuyo (UNCuyo). Facultad de Ciencias Exactas y Naturales (FCEN). Laboratorio de Química Analítica para Investigación y Desarrollo (QUIANID); Argentina Fil: Lemos, Aldana. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Interdisciplinario de Ciencias Básicas (ICB); Argentina Fil: Lemos, Aldana. Universidad Nacional de Cuyo (UNCuyo). Instituto Interdisciplinario de Ciencias Básicas (ICB); Argentina Fil: Soto Vargas, Verónica Carolina. Universidad Nacional de Cuyo (UNCuyo). Facultad de Ciencias Agrarias (FCA). Instituto de Biología Agrícola de Mendoza (IBAM); Argentina Fil: Soto Vargas, Verónica Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto de Biología Agrícola de Mendoza (IBAM); Argentina Fil: Wuilloud, Rodolfo Germán. Universidad Nacional de Cuyo (UNCuyo). Facultad de Ciencias Exactas y Naturales (FCEN). Laboratorio de Química Analítica para Investigación y Desarrollo (QUIANID); Argentina Fil: Wuilloud, Rodolfo Germán. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Instituto Interdisciplinario de Ciencias Básicas (ICB); Argentina Fil: Wuilloud, Rodolfo Germán. Universidad Nacional de Cuyo (UNCuyo). Instituto Interdisciplinario de Ciencias Básicas (ICB); Argentina Fil: González, Roxana Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina Fil: González, Roxana Elizabeth. Universidad Nacional de Cuyo (UNCuyo). Facultad de Ciencias Exactas y Naturales (FCEN); Argentina 2024-11-29T12:39:27Z 2024-11-29T12:39:27Z 2024-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/20392 https://link.springer.com/article/10.1007/s12161-024-02656-z 1936-9751 1936-976X (online) https://doi.org/10.1007/s12161-024-02656-z eng info:eu-repograntAgreement/INTA/2019-PD-E7-I152-001/2019-PD-E7-I152-001/AR./Alimentos nutracéuticos, funcionales o para regímenes especiales. info:eu-repograntAgreement/INTA/2019-PE-E7-I517-001/2019-PE-E7-I517-001, Calidad nutricional y sensorial de alimentos y aptitud tecnológica de materia prima asociada a sistemas y territorios productivos con foco en las demandas del consumidor. info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Food Analytical Methods 17 (12) : 1281–1291. (August 2024) |
| spellingShingle | Compuestos Bioactivos Lechugas Lactuca sativa Fitoquímicos Diseño Experimental Bioactive Compounds Lettuces Phytochemicals Experimental Design Lactuca sativa L. Quantification Lemos, Aldana Ayelén Soto Vargas, Verónica Carolina Wuilloud, Rodolfo Germán Gonzalez, Roxana Elizabeth Screening of bioactive compounds in lettuce: multivariate optimization of an ultrasound‑assisted solid–liquid extraction procedure |
| title | Screening of bioactive compounds in lettuce: multivariate optimization of an ultrasound‑assisted solid–liquid extraction procedure |
| title_full | Screening of bioactive compounds in lettuce: multivariate optimization of an ultrasound‑assisted solid–liquid extraction procedure |
| title_fullStr | Screening of bioactive compounds in lettuce: multivariate optimization of an ultrasound‑assisted solid–liquid extraction procedure |
| title_full_unstemmed | Screening of bioactive compounds in lettuce: multivariate optimization of an ultrasound‑assisted solid–liquid extraction procedure |
| title_short | Screening of bioactive compounds in lettuce: multivariate optimization of an ultrasound‑assisted solid–liquid extraction procedure |
| title_sort | screening of bioactive compounds in lettuce multivariate optimization of an ultrasound assisted solid liquid extraction procedure |
| topic | Compuestos Bioactivos Lechugas Lactuca sativa Fitoquímicos Diseño Experimental Bioactive Compounds Lettuces Phytochemicals Experimental Design Lactuca sativa L. Quantification |
| url | http://hdl.handle.net/20.500.12123/20392 https://link.springer.com/article/10.1007/s12161-024-02656-z https://doi.org/10.1007/s12161-024-02656-z |
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