Cover Image
QR Code

A haplotype at intron 8 of PTPRT gene is associated with resistance to Brucella infection in Argentinian creole goats

Brucellosis is the leading zoonosis on a worldwide scale and constitutes a major public health threat in many regions of the world. Several molecular markers associated with natural resistance to intracellular bacterial infection have been identified. Recently seven single-nucleotide polymorphisms (...

Full description

Main Authors: Rossi, Ursula, Hasenauer, Flavia Carolina, Caffaro, Marí­a Eugenia, Neumann, Roberto Daniel, Salatin, Antonio Omar, Poli, Mario Andres, Rossetti, Carlos Alberto
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: 2018
Subjects:
Caprinos
Genética
Brucella
Enfermedades de los Animales
Brucelosis
Goats
Genetics
Animal Diseases
Brucellosis
Argentina
Online Access:http://hdl.handle.net/20.500.12123/1992
https://www.sciencedirect.com/science/article/pii/S0378113517302341
https://doi.org/10.1016/j.vetmic.2017.06.001
Summary:Brucellosis is the leading zoonosis on a worldwide scale and constitutes a major public health threat in many regions of the world. Several molecular markers associated with natural resistance to intracellular bacterial infection have been identified. Recently seven single-nucleotide polymorphisms (SNPs) located in the PTPRT gene were associated with resistance to Mycobacterium bovis infection in cattle. Here, we perform a case-control study to test if polymorphisms at PTPRT intron 8 might influence the resistance or susceptibility to Brucella infection in goats. DNA samples from 22 seropositive (cases) and 22 seronegative (controls) for brucellosis, unrelated female creole goats, were included in the present study. Four previously reported polymorphisms (SNP1: rs643551276, SNP2: rs651618967, SNP3: rs662137815 and SNP4: rs657542977) and a new SNP (SNP5: chr13: 691695526) were detected by PCR-DNA sequencing method. Genotypic and allelic frequencies differed significantly between cases and controls at SNPs 1, 2, 4 and 5 (p ≤0.001). Indeed, the SNP1 TT, SNP2 TT, SNP4 CC and SNP5 TT genotypes were associated with absence of Brucella-specific antibodies (ORs =0.019 to 0.045). Moreover, haplotype association analysis revealed a significant association of the TTCCT haplotype with protection to Brucella infection (p ≤1 × 10−4; OR =18), including the major allelic variants associated with resistance. These results represent the first evidence of genetic association between polymorphisms in the PTPRT gene and absence of brucellosis in goats.

Similar Items