A haplotype at intron 8 of PTPRT gene is associated with resistance to Brucella infection in Argentinian creole goats
Brucellosis is the leading zoonosis on a worldwide scale and constitutes a major public health threat in many regions of the world. Several molecular markers associated with natural resistance to intracellular bacterial infection have been identified. Recently seven single-nucleotide polymorphisms (...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2018
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1992 https://www.sciencedirect.com/science/article/pii/S0378113517302341 https://doi.org/10.1016/j.vetmic.2017.06.001 |
| _version_ | 1855034886255017984 |
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| author | Rossi, Ursula Hasenauer, Flavia Carolina Caffaro, María Eugenia Neumann, Roberto Daniel Salatin, Antonio Omar Poli, Mario Andres Rossetti, Carlos Alberto |
| author_browse | Caffaro, María Eugenia Hasenauer, Flavia Carolina Neumann, Roberto Daniel Poli, Mario Andres Rossetti, Carlos Alberto Rossi, Ursula Salatin, Antonio Omar |
| author_facet | Rossi, Ursula Hasenauer, Flavia Carolina Caffaro, María Eugenia Neumann, Roberto Daniel Salatin, Antonio Omar Poli, Mario Andres Rossetti, Carlos Alberto |
| author_sort | Rossi, Ursula |
| collection | INTA Digital |
| description | Brucellosis is the leading zoonosis on a worldwide scale and constitutes a major public health threat in many regions of the world. Several molecular markers associated with natural resistance to intracellular bacterial infection have been identified. Recently seven single-nucleotide polymorphisms (SNPs) located in the PTPRT gene were associated with resistance to Mycobacterium bovis infection in cattle. Here, we perform a case-control study to test if polymorphisms at PTPRT intron 8 might influence the resistance or susceptibility to Brucella infection in goats. DNA samples from 22 seropositive (cases) and 22 seronegative (controls) for brucellosis, unrelated female creole goats, were included in the present study. Four previously reported polymorphisms (SNP1: rs643551276, SNP2: rs651618967, SNP3: rs662137815 and SNP4: rs657542977) and a new SNP (SNP5: chr13: 691695526) were detected by PCR-DNA sequencing method. Genotypic and allelic frequencies differed significantly between cases and controls at SNPs 1, 2, 4 and 5 (p ≤0.001). Indeed, the SNP1 TT, SNP2 TT, SNP4 CC and SNP5 TT genotypes were associated with absence of Brucella-specific antibodies (ORs =0.019 to 0.045).
Moreover, haplotype association analysis revealed a significant association of the TTCCT haplotype with protection to Brucella infection (p ≤1 × 10−4; OR =18), including the major allelic variants associated with resistance.
These results represent the first evidence of genetic association between polymorphisms in the PTPRT gene and absence of brucellosis in goats. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1992 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| record_format | dspace |
| spelling | INTA19922019-09-11T17:50:35Z A haplotype at intron 8 of PTPRT gene is associated with resistance to Brucella infection in Argentinian creole goats Rossi, Ursula Hasenauer, Flavia Carolina Caffaro, María Eugenia Neumann, Roberto Daniel Salatin, Antonio Omar Poli, Mario Andres Rossetti, Carlos Alberto Caprinos Genética Brucella Enfermedades de los Animales Brucelosis Goats Genetics Animal Diseases Brucellosis Argentina Brucellosis is the leading zoonosis on a worldwide scale and constitutes a major public health threat in many regions of the world. Several molecular markers associated with natural resistance to intracellular bacterial infection have been identified. Recently seven single-nucleotide polymorphisms (SNPs) located in the PTPRT gene were associated with resistance to Mycobacterium bovis infection in cattle. Here, we perform a case-control study to test if polymorphisms at PTPRT intron 8 might influence the resistance or susceptibility to Brucella infection in goats. DNA samples from 22 seropositive (cases) and 22 seronegative (controls) for brucellosis, unrelated female creole goats, were included in the present study. Four previously reported polymorphisms (SNP1: rs643551276, SNP2: rs651618967, SNP3: rs662137815 and SNP4: rs657542977) and a new SNP (SNP5: chr13: 691695526) were detected by PCR-DNA sequencing method. Genotypic and allelic frequencies differed significantly between cases and controls at SNPs 1, 2, 4 and 5 (p ≤0.001). Indeed, the SNP1 TT, SNP2 TT, SNP4 CC and SNP5 TT genotypes were associated with absence of Brucella-specific antibodies (ORs =0.019 to 0.045). Moreover, haplotype association analysis revealed a significant association of the TTCCT haplotype with protection to Brucella infection (p ≤1 × 10−4; OR =18), including the major allelic variants associated with resistance. These results represent the first evidence of genetic association between polymorphisms in the PTPRT gene and absence of brucellosis in goats. Inst. de Patobiología Fil: Rossi, Ursula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Hasenauer, Flavia Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Caffaro, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Neumann, Roberto Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Salatin, Antonio Omar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina Fil: Poli, Mario Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina 2018-03-08T12:24:27Z 2018-03-08T12:24:27Z 2017-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1992 https://www.sciencedirect.com/science/article/pii/S0378113517302341 0378-1135 https://doi.org/10.1016/j.vetmic.2017.06.001 eng info:eu-repo/semantics/restrictedAccess application/pdf Veterinary microbiology 207 : 133-137. (August 2017) |
| spellingShingle | Caprinos Genética Brucella Enfermedades de los Animales Brucelosis Goats Genetics Animal Diseases Brucellosis Argentina Rossi, Ursula Hasenauer, Flavia Carolina Caffaro, María Eugenia Neumann, Roberto Daniel Salatin, Antonio Omar Poli, Mario Andres Rossetti, Carlos Alberto A haplotype at intron 8 of PTPRT gene is associated with resistance to Brucella infection in Argentinian creole goats |
| title | A haplotype at intron 8 of PTPRT gene is associated with resistance to Brucella infection in Argentinian creole goats |
| title_full | A haplotype at intron 8 of PTPRT gene is associated with resistance to Brucella infection in Argentinian creole goats |
| title_fullStr | A haplotype at intron 8 of PTPRT gene is associated with resistance to Brucella infection in Argentinian creole goats |
| title_full_unstemmed | A haplotype at intron 8 of PTPRT gene is associated with resistance to Brucella infection in Argentinian creole goats |
| title_short | A haplotype at intron 8 of PTPRT gene is associated with resistance to Brucella infection in Argentinian creole goats |
| title_sort | haplotype at intron 8 of ptprt gene is associated with resistance to brucella infection in argentinian creole goats |
| topic | Caprinos Genética Brucella Enfermedades de los Animales Brucelosis Goats Genetics Animal Diseases Brucellosis Argentina |
| url | http://hdl.handle.net/20.500.12123/1992 https://www.sciencedirect.com/science/article/pii/S0378113517302341 https://doi.org/10.1016/j.vetmic.2017.06.001 |
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