Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes
Modern sugarcane (Saccharum spp.) cultivars originated from an intricate hybridization process, resulting in complex polyploid and aneuploid genomes. Flow cytometry (FCM), which has emerged as a precise and fast method for research on genome size in plants, has potential to unveil key aspects of sug...
| Main Authors: | , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
Wiley
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/18773 https://onlinelibrary.wiley.com/doi/10.1111/pbr.13203 https://doi.org/10.1111/pbr.13203 |
| _version_ | 1855486238966939648 |
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| author | Garcia, José María Andrada, Aldo R. Erazzu, Luis Ernesto Acevedo, Alberto |
| author_browse | Acevedo, Alberto Andrada, Aldo R. Erazzu, Luis Ernesto Garcia, José María |
| author_facet | Garcia, José María Andrada, Aldo R. Erazzu, Luis Ernesto Acevedo, Alberto |
| author_sort | Garcia, José María |
| collection | INTA Digital |
| description | Modern sugarcane (Saccharum spp.) cultivars originated from an intricate hybridization process, resulting in complex polyploid and aneuploid genomes. Flow cytometry (FCM), which has emerged as a precise and fast method for research on genome size in plants, has potential to unveil key aspects of sugarcane cytogenetics. In this study, FCM probes were conducted to estimate 2C DNA content in 175 Saccharum genotypes embracing phenotypically contrasting canes (commercial cultivars, energy-cane hybrids, and a Saccharum spontaneum accession) and full-sib sugarcane families. FCM-mediated DNA content estimates exhibited high accuracy in predicting chromosome numbers, offering a time-efficient alternative to classical cytogenetic procedure. Low-magnitude correlations detected between DNA content and phenotypic traits suggest that indirect selection of agronomic traits using FCM-mediated DNA content estimates may not be efficient. DNA content variation patterns in sugarcane families varied according to the crossing, and revealed transgressive variations associated with rampant aneuploidy. Altogether, the findings uncover that loss or gain of genetic material constitutes a source of high genetic variability that may substantially impact on crop breeding. |
| format | Artículo |
| id | INTA18773 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA187732024-08-02T13:18:30Z Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes Garcia, José María Andrada, Aldo R. Erazzu, Luis Ernesto Acevedo, Alberto Caña de Azúcar Variación Genética Genotipos Citometría de Flujo (Células) Poliploidia Variación Fenotípica Sugar Cane Genetic Variation Genotypes Flow Cytometry Polyploidy Phenotypic Variation Saccharum Modern sugarcane (Saccharum spp.) cultivars originated from an intricate hybridization process, resulting in complex polyploid and aneuploid genomes. Flow cytometry (FCM), which has emerged as a precise and fast method for research on genome size in plants, has potential to unveil key aspects of sugarcane cytogenetics. In this study, FCM probes were conducted to estimate 2C DNA content in 175 Saccharum genotypes embracing phenotypically contrasting canes (commercial cultivars, energy-cane hybrids, and a Saccharum spontaneum accession) and full-sib sugarcane families. FCM-mediated DNA content estimates exhibited high accuracy in predicting chromosome numbers, offering a time-efficient alternative to classical cytogenetic procedure. Low-magnitude correlations detected between DNA content and phenotypic traits suggest that indirect selection of agronomic traits using FCM-mediated DNA content estimates may not be efficient. DNA content variation patterns in sugarcane families varied according to the crossing, and revealed transgressive variations associated with rampant aneuploidy. Altogether, the findings uncover that loss or gain of genetic material constitutes a source of high genetic variability that may substantially impact on crop breeding. EEA Famaillá Fil: García, José María. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina Fil: Andrada, Aldo R. Fundación M. Lillo. Instituto de Genética y Microbiología. Area Biología Integrativa; Argentina Fil: Erazzú, Luis E. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina. Fil: Acevedo, Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina 2024-08-02T13:13:51Z 2024-08-02T13:13:51Z 2024-07 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18773 https://onlinelibrary.wiley.com/doi/10.1111/pbr.13203 0179-9541 1439-0523 https://doi.org/10.1111/pbr.13203 eng info:eu-repograntAgreement/INTA/2023-PD-L01-I071, Mejoramiento genético de los Cultivos Industriales y estrategias alternativas para la obtención de biotipos adaptados a los nuevos escenarios y al cambio climático info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley Plant Breeding : 1-13. (First published: 29 July 2024) |
| spellingShingle | Caña de Azúcar Variación Genética Genotipos Citometría de Flujo (Células) Poliploidia Variación Fenotípica Sugar Cane Genetic Variation Genotypes Flow Cytometry Polyploidy Phenotypic Variation Saccharum Garcia, José María Andrada, Aldo R. Erazzu, Luis Ernesto Acevedo, Alberto Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes |
| title | Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes |
| title_full | Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes |
| title_fullStr | Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes |
| title_full_unstemmed | Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes |
| title_short | Genome size and chromosome number variation in sugarcane (Saccharum spp.) families and phenotypically contrasting Saccharum genotypes |
| title_sort | genome size and chromosome number variation in sugarcane saccharum spp families and phenotypically contrasting saccharum genotypes |
| topic | Caña de Azúcar Variación Genética Genotipos Citometría de Flujo (Células) Poliploidia Variación Fenotípica Sugar Cane Genetic Variation Genotypes Flow Cytometry Polyploidy Phenotypic Variation Saccharum |
| url | http://hdl.handle.net/20.500.12123/18773 https://onlinelibrary.wiley.com/doi/10.1111/pbr.13203 https://doi.org/10.1111/pbr.13203 |
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