Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds
DNA glycosylases initiate the base excision repair (BER) pathway by catalyzing the removal of damaged or mismatched bases from DNA. The Arabidopsis DNA glycosylase methyl-CpG-binding domain protein 4 like (MBD4L) is a nuclear enzyme triggering BER in response to the genotoxic agents 5-fluorouracil a...
| Main Authors: | , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Wiley
2024
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/18506 https://onlinelibrary.wiley.com/doi/10.1111/tpj.16907 https://doi.org/10.1111/tpj.16907 |
| _version_ | 1855037869531332608 |
|---|---|
| author | Lescano Lopez, Carlos Ignacio Torres, José Roberto Cecchini, Nicolás Miguel Alvarez, María Elena |
| author_browse | Alvarez, María Elena Cecchini, Nicolás Miguel Lescano Lopez, Carlos Ignacio Torres, José Roberto |
| author_facet | Lescano Lopez, Carlos Ignacio Torres, José Roberto Cecchini, Nicolás Miguel Alvarez, María Elena |
| author_sort | Lescano Lopez, Carlos Ignacio |
| collection | INTA Digital |
| description | DNA glycosylases initiate the base excision repair (BER) pathway by catalyzing the removal of damaged or mismatched bases from DNA. The Arabidopsis DNA glycosylase methyl-CpG-binding domain protein 4 like (MBD4L) is a nuclear enzyme triggering BER in response to the genotoxic agents 5-fluorouracil and 5-bromouracil. To date, the involvement of MBD4L in plant physiological processes has not been analyzed. To address this, we studied the enzyme functions in seeds. We found that imbibition induced the MBD4L gene expression by generating two alternative transcripts, MBD4L.3 and MBD4L.4. Gene activation was stronger in aged than in non-aged seeds. Seeds from mbd4l-1 mutants displayed germination failures when maintained under control or ageing conditions, while 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 seeds reversed these phenotypes. Seed nuclear DNA repair, assessed by comet assays, was exacerbated in an MBD4L-dependent manner at 24 h post-imbibition. Under this condition, the BER genes ARP, APE1L, and LIG1 showed higher expression in 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 than in mbd4l-1 seeds, suggesting that these components could coordinate with MBD4L to repair damaged DNA bases in seeds. Interestingly, the ATM, ATR, BRCA1, RAD51, and WEE1 genes associated with the DNA damage response (DDR) pathway were activated in mbd4l-1, but not in 35S:MBD4L.3/mbd4l-1 or 35S:MBD4L.4/mbd4l-1 seeds. These results indicate that MBD4L is a key enzyme of a BER cascade that operates during seed imbibition, whose deficiency would cause genomic damage detected by DDR, generating a delay or reduction in germination. |
| format | info:ar-repo/semantics/artículo |
| id | INTA18506 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Wiley |
| publisherStr | Wiley |
| record_format | dspace |
| spelling | INTA185062024-07-15T12:20:58Z Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds Lescano Lopez, Carlos Ignacio Torres, José Roberto Cecchini, Nicolás Miguel Alvarez, María Elena Seed Germination DNA Damage Germinación de las Semillas Daño al ADN Base Excision Repair DNA Glycosylases Seed Ageing DNA Damage Responses MBD4L DNA glycosylases initiate the base excision repair (BER) pathway by catalyzing the removal of damaged or mismatched bases from DNA. The Arabidopsis DNA glycosylase methyl-CpG-binding domain protein 4 like (MBD4L) is a nuclear enzyme triggering BER in response to the genotoxic agents 5-fluorouracil and 5-bromouracil. To date, the involvement of MBD4L in plant physiological processes has not been analyzed. To address this, we studied the enzyme functions in seeds. We found that imbibition induced the MBD4L gene expression by generating two alternative transcripts, MBD4L.3 and MBD4L.4. Gene activation was stronger in aged than in non-aged seeds. Seeds from mbd4l-1 mutants displayed germination failures when maintained under control or ageing conditions, while 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 seeds reversed these phenotypes. Seed nuclear DNA repair, assessed by comet assays, was exacerbated in an MBD4L-dependent manner at 24 h post-imbibition. Under this condition, the BER genes ARP, APE1L, and LIG1 showed higher expression in 35S:MBD4L.3/mbd4l-1 and 35S:MBD4L.4/mbd4l-1 than in mbd4l-1 seeds, suggesting that these components could coordinate with MBD4L to repair damaged DNA bases in seeds. Interestingly, the ATM, ATR, BRCA1, RAD51, and WEE1 genes associated with the DNA damage response (DDR) pathway were activated in mbd4l-1, but not in 35S:MBD4L.3/mbd4l-1 or 35S:MBD4L.4/mbd4l-1 seeds. These results indicate that MBD4L is a key enzyme of a BER cascade that operates during seed imbibition, whose deficiency would cause genomic damage detected by DDR, generating a delay or reduction in germination. Instituto de Fisiología y Recursos Genéticos Vegetales Fil: Lescano Lopez, Carlos Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina Fil: Lescano Lopez, Carlos Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Lescano Lopez, Carlos Ignacio. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina Fil: Torres, José Roberto. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC). Departamento de Química Biológica Ranwel Caputto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cecchini, Nicolás Miguel. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC). Departamento de Química Biológica Ranwel Caputto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alvarez, María Elena. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Centro de Investigaciones en Química Biológica de Córdoba (CIQUIBIC). Departamento de Química Biológica Ranwel Caputto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-07-15T11:40:58Z 2024-07-15T11:40:58Z 2024-07-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18506 https://onlinelibrary.wiley.com/doi/10.1111/tpj.16907 0960-7412 1365-313X (online) https://doi.org/10.1111/tpj.16907 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Wiley The Plant Journal : 1-12 (First published: 04 July 2024) |
| spellingShingle | Seed Germination DNA Damage Germinación de las Semillas Daño al ADN Base Excision Repair DNA Glycosylases Seed Ageing DNA Damage Responses MBD4L Lescano Lopez, Carlos Ignacio Torres, José Roberto Cecchini, Nicolás Miguel Alvarez, María Elena Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds |
| title | Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds |
| title_full | Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds |
| title_fullStr | Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds |
| title_full_unstemmed | Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds |
| title_short | Arabidopsis DNA glycosylase MBD4L improves recovery of aged seeds |
| title_sort | arabidopsis dna glycosylase mbd4l improves recovery of aged seeds |
| topic | Seed Germination DNA Damage Germinación de las Semillas Daño al ADN Base Excision Repair DNA Glycosylases Seed Ageing DNA Damage Responses MBD4L |
| url | http://hdl.handle.net/20.500.12123/18506 https://onlinelibrary.wiley.com/doi/10.1111/tpj.16907 https://doi.org/10.1111/tpj.16907 |
| work_keys_str_mv | AT lescanolopezcarlosignacio arabidopsisdnaglycosylasembd4limprovesrecoveryofagedseeds AT torresjoseroberto arabidopsisdnaglycosylasembd4limprovesrecoveryofagedseeds AT cecchininicolasmiguel arabidopsisdnaglycosylasembd4limprovesrecoveryofagedseeds AT alvarezmariaelena arabidopsisdnaglycosylasembd4limprovesrecoveryofagedseeds |