Heat treatment of peach fruit: Modifications in the extracellular compartment and identification of novel extracellular proteins
Ripening of peach (Prunus persica L. Batsch) fruit is accompanied by dramatic cell wall changes that lead to softening. Post-harvest heat treatment is effective in delaying softening and preventing some chilling injury symptoms that this fruit exhibits after storage at low temperatures. In the prese...
| Autores principales: | , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/18384 https://www.sciencedirect.com/science/article/pii/S0981942812002008 https://doi.org/10.1016/j.plaphy.2012.07.021 |
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| author | Bustamante, Claudia Anabel Budde, Claudio Olaf Borsani, Julia Lombardo, Verónica Andrea Lauxmann, Martin Alexander Andreo, Carlos Santiago Lara, María Valeria Drincovich, María Fabiana |
| author_browse | Andreo, Carlos Santiago Borsani, Julia Budde, Claudio Olaf Bustamante, Claudia Anabel Drincovich, María Fabiana Lara, María Valeria Lauxmann, Martin Alexander Lombardo, Verónica Andrea |
| author_facet | Bustamante, Claudia Anabel Budde, Claudio Olaf Borsani, Julia Lombardo, Verónica Andrea Lauxmann, Martin Alexander Andreo, Carlos Santiago Lara, María Valeria Drincovich, María Fabiana |
| author_sort | Bustamante, Claudia Anabel |
| collection | INTA Digital |
| description | Ripening of peach (Prunus persica L. Batsch) fruit is accompanied by dramatic cell wall changes that lead to softening. Post-harvest heat treatment is effective in delaying softening and preventing some chilling injury symptoms that this fruit exhibits after storage at low temperatures. In the present work, the levels of twelve transcripts encoding proteins involved in cell wall metabolism, as well as the differential extracellular proteome, were examined after a post-harvest heat treatment (HT; 39 °C for 3 days) of “Dixiland” peach fruit. A typical softening behaviour, in correlation with an increase in 1-aminocyclopropane-1-carboxylic acid oxidase-1 (PpACO1), was observed for peach maintained at 20 °C for 3 days (R3). Six transcripts encoding proteins involved in cell wall metabolism significantly increased in R3 with respect to peach at harvest, while six showed no modification or even decreased. In contrast, after HT, fruit maintained their firmness, exhibiting low PpACO1 level and significant lower levels of the twelve cell wall-modifying genes than in R3. Differential proteomic analysis of apoplastic proteins during softening and after HT revealed a significant decrease of DUF642 proteins after HT; as well as an increase of glyceraldehyde-3-phosphate dehydrogenase (GAPC) after softening. The presence of GAPC in the peach extracellular matrix was further confirmed by in situ immunolocalization and transient expression in tomato fruit. Though further studies are required to establish the function of DUF642 and GAPC in the apoplast, this study contributes to a deeper understanding of the events during peach softening and after HT with a focus on this key compartment. |
| format | Artículo |
| id | INTA18384 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA183842024-07-04T13:28:34Z Heat treatment of peach fruit: Modifications in the extracellular compartment and identification of novel extracellular proteins Bustamante, Claudia Anabel Budde, Claudio Olaf Borsani, Julia Lombardo, Verónica Andrea Lauxmann, Martin Alexander Andreo, Carlos Santiago Lara, María Valeria Drincovich, María Fabiana Frutas de Hueso Durazno Prunus persica Pared Celular Tratamiento Térmico Fisiología Postcosecha Stone fruits Peaches Cell Walls Heat Treatment Postharvest Physiology DUF642 GAPC Ripening of peach (Prunus persica L. Batsch) fruit is accompanied by dramatic cell wall changes that lead to softening. Post-harvest heat treatment is effective in delaying softening and preventing some chilling injury symptoms that this fruit exhibits after storage at low temperatures. In the present work, the levels of twelve transcripts encoding proteins involved in cell wall metabolism, as well as the differential extracellular proteome, were examined after a post-harvest heat treatment (HT; 39 °C for 3 days) of “Dixiland” peach fruit. A typical softening behaviour, in correlation with an increase in 1-aminocyclopropane-1-carboxylic acid oxidase-1 (PpACO1), was observed for peach maintained at 20 °C for 3 days (R3). Six transcripts encoding proteins involved in cell wall metabolism significantly increased in R3 with respect to peach at harvest, while six showed no modification or even decreased. In contrast, after HT, fruit maintained their firmness, exhibiting low PpACO1 level and significant lower levels of the twelve cell wall-modifying genes than in R3. Differential proteomic analysis of apoplastic proteins during softening and after HT revealed a significant decrease of DUF642 proteins after HT; as well as an increase of glyceraldehyde-3-phosphate dehydrogenase (GAPC) after softening. The presence of GAPC in the peach extracellular matrix was further confirmed by in situ immunolocalization and transient expression in tomato fruit. Though further studies are required to establish the function of DUF642 and GAPC in the apoplast, this study contributes to a deeper understanding of the events during peach softening and after HT with a focus on this key compartment. EEA San Pedro, INTA Fil: Bustamante, Claudia Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Bustamante, Claudia Anabel. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina Fil: Borsani, Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Borsani, Julia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Lombardo, Verónica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Lombardo, Verónica Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Lauxmann, Martin Alexander. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Lauxmann, Martin Alexander. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Andreo, Carlos Santiago. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Lara, María Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Lara, María Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina Fil: Drincovich, María Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Drincovich, María Fabiana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos (CEFOBI); Argentina 2024-07-04T13:23:49Z 2024-07-04T13:23:49Z 2012-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18384 https://www.sciencedirect.com/science/article/pii/S0981942812002008 0981-9428 https://doi.org/10.1016/j.plaphy.2012.07.021 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Plant Physiology and Biochemistry 60 : 35-45 (nov. 2012). |
| spellingShingle | Frutas de Hueso Durazno Prunus persica Pared Celular Tratamiento Térmico Fisiología Postcosecha Stone fruits Peaches Cell Walls Heat Treatment Postharvest Physiology DUF642 GAPC Bustamante, Claudia Anabel Budde, Claudio Olaf Borsani, Julia Lombardo, Verónica Andrea Lauxmann, Martin Alexander Andreo, Carlos Santiago Lara, María Valeria Drincovich, María Fabiana Heat treatment of peach fruit: Modifications in the extracellular compartment and identification of novel extracellular proteins |
| title | Heat treatment of peach fruit: Modifications in the extracellular compartment and identification of novel extracellular proteins |
| title_full | Heat treatment of peach fruit: Modifications in the extracellular compartment and identification of novel extracellular proteins |
| title_fullStr | Heat treatment of peach fruit: Modifications in the extracellular compartment and identification of novel extracellular proteins |
| title_full_unstemmed | Heat treatment of peach fruit: Modifications in the extracellular compartment and identification of novel extracellular proteins |
| title_short | Heat treatment of peach fruit: Modifications in the extracellular compartment and identification of novel extracellular proteins |
| title_sort | heat treatment of peach fruit modifications in the extracellular compartment and identification of novel extracellular proteins |
| topic | Frutas de Hueso Durazno Prunus persica Pared Celular Tratamiento Térmico Fisiología Postcosecha Stone fruits Peaches Cell Walls Heat Treatment Postharvest Physiology DUF642 GAPC |
| url | http://hdl.handle.net/20.500.12123/18384 https://www.sciencedirect.com/science/article/pii/S0981942812002008 https://doi.org/10.1016/j.plaphy.2012.07.021 |
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