Xylanase production by cellulomonas phragmiteti using lignocellulosic waste materials
Lignocellulosic biomass holds promise as a renewable feedstock for various applications, but its efficient conversion requires cost-effective degradation strategies. The main objective of this study was to investigate the effect of the growth conditions of Cellulomonas phragmiteti in the production...
| Autores principales: | , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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MDPI
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/18363 https://www.mdpi.com/2227-9717/12/2/258 https://doi.org/10.3390/pr12020258 |
| _version_ | 1855486161295769600 |
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| author | Buda, Kata Fekete, Tünde Ontañon, Ornella Mailen Campos, Eleonora Fehér, Csaba |
| author_browse | Buda, Kata Campos, Eleonora Fehér, Csaba Fekete, Tünde Ontañon, Ornella Mailen |
| author_facet | Buda, Kata Fekete, Tünde Ontañon, Ornella Mailen Campos, Eleonora Fehér, Csaba |
| author_sort | Buda, Kata |
| collection | INTA Digital |
| description | Lignocellulosic biomass holds promise as a renewable feedstock for various applications, but its efficient conversion requires cost-effective degradation strategies. The main objective of this study was to investigate the effect of the growth conditions of Cellulomonas phragmiteti in the production of (hemi)cellulosic supernatants. To meet this aim, different lignocellulosic residues were used as carbon sources for growth using defined mineral or nutritive culture media. Cell-free culture supernatants with xylanolytic activity were produced in all the conditions evaluated, but the highest xylanase activity (15.3 U/mL) was achieved in Luria–Bertani (LB) medium containing 1% waste paper. Under these conditions, almost negligible β-glucosidase, cellobiohydrolase, β-xylosidase, and α-arabinofuranosidase activity was detected. The xylanolytic supernatant showed tolerance to salt and displayed maximal catalytic efficiency at pH 6 and 45 °C, along with good activity in the ranges of 45–55 °C and pH 5–8. As it showed good stability at 45 °C, the supernatant was employed for the hydrolysis of birchwood xylan (50 g/L) under optimal conditions, releasing 10.7 g/L xylose in 72 h. Thus, C. phragmiteti was found to produce a xylanolytic enzymatic supernatant efficiently by utilizing the cheap and abundant lignocellulosic residue of waste paper, and the produced supernatant has promising attributes for industrial applications. |
| format | Artículo |
| id | INTA18363 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA183632024-07-03T09:47:44Z Xylanase production by cellulomonas phragmiteti using lignocellulosic waste materials Buda, Kata Fekete, Tünde Ontañon, Ornella Mailen Campos, Eleonora Fehér, Csaba Cellulomonas Lignocellulose Wastes Enzymes Lignocelulosa Desechos Enzimas Cellulomonas phragmiteti Producción de Xilanasa Xylanase Production Lignocellulosic biomass holds promise as a renewable feedstock for various applications, but its efficient conversion requires cost-effective degradation strategies. The main objective of this study was to investigate the effect of the growth conditions of Cellulomonas phragmiteti in the production of (hemi)cellulosic supernatants. To meet this aim, different lignocellulosic residues were used as carbon sources for growth using defined mineral or nutritive culture media. Cell-free culture supernatants with xylanolytic activity were produced in all the conditions evaluated, but the highest xylanase activity (15.3 U/mL) was achieved in Luria–Bertani (LB) medium containing 1% waste paper. Under these conditions, almost negligible β-glucosidase, cellobiohydrolase, β-xylosidase, and α-arabinofuranosidase activity was detected. The xylanolytic supernatant showed tolerance to salt and displayed maximal catalytic efficiency at pH 6 and 45 °C, along with good activity in the ranges of 45–55 °C and pH 5–8. As it showed good stability at 45 °C, the supernatant was employed for the hydrolysis of birchwood xylan (50 g/L) under optimal conditions, releasing 10.7 g/L xylose in 72 h. Thus, C. phragmiteti was found to produce a xylanolytic enzymatic supernatant efficiently by utilizing the cheap and abundant lignocellulosic residue of waste paper, and the produced supernatant has promising attributes for industrial applications. Instituto de Biotecnología Fil: Buda, Kata. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungría Fil: Fekete, Tünde. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungría Fil: Ontañon, Ornella Mailen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ontañon, Ornella Mailen. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fehér, Csaba. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungría 2024-07-03T09:42:37Z 2024-07-03T09:42:37Z 2024-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18363 https://www.mdpi.com/2227-9717/12/2/258 2227-9717 https://doi.org/10.3390/pr12020258 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Processes 12 (2) : 258 (February 2024) |
| spellingShingle | Cellulomonas Lignocellulose Wastes Enzymes Lignocelulosa Desechos Enzimas Cellulomonas phragmiteti Producción de Xilanasa Xylanase Production Buda, Kata Fekete, Tünde Ontañon, Ornella Mailen Campos, Eleonora Fehér, Csaba Xylanase production by cellulomonas phragmiteti using lignocellulosic waste materials |
| title | Xylanase production by cellulomonas phragmiteti using lignocellulosic waste materials |
| title_full | Xylanase production by cellulomonas phragmiteti using lignocellulosic waste materials |
| title_fullStr | Xylanase production by cellulomonas phragmiteti using lignocellulosic waste materials |
| title_full_unstemmed | Xylanase production by cellulomonas phragmiteti using lignocellulosic waste materials |
| title_short | Xylanase production by cellulomonas phragmiteti using lignocellulosic waste materials |
| title_sort | xylanase production by cellulomonas phragmiteti using lignocellulosic waste materials |
| topic | Cellulomonas Lignocellulose Wastes Enzymes Lignocelulosa Desechos Enzimas Cellulomonas phragmiteti Producción de Xilanasa Xylanase Production |
| url | http://hdl.handle.net/20.500.12123/18363 https://www.mdpi.com/2227-9717/12/2/258 https://doi.org/10.3390/pr12020258 |
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