Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi
Ten Argentinean Waitea circinata-like isolates (Corticiales, Basidiomycota) obtained from soil samples were identified to species level based on morphological features and molecular tools, and tested in order to explore their biological control abilities. Eight of them were identified as Waitea arva...
| Autores principales: | , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2024
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/18232 https://link.springer.com/article/10.1007/s11557-024-01944-6 https://doi.org/10.1007/s11557-024-01944-6 |
| _version_ | 1855037815869407232 |
|---|---|
| author | Melignani, Eliana Guidobono, Juan Santiago Rojo, Rodrigo Levin, Laura Barrera, Viviana Andrea |
| author_browse | Barrera, Viviana Andrea Guidobono, Juan Santiago Levin, Laura Melignani, Eliana Rojo, Rodrigo |
| author_facet | Melignani, Eliana Guidobono, Juan Santiago Rojo, Rodrigo Levin, Laura Barrera, Viviana Andrea |
| author_sort | Melignani, Eliana |
| collection | INTA Digital |
| description | Ten Argentinean Waitea circinata-like isolates (Corticiales, Basidiomycota) obtained from soil samples were identified to species level based on morphological features and molecular tools, and tested in order to explore their biological control abilities. Eight of them were identified as Waitea arvalis and two as W. circinata. Waitea arvalis isolates were tested as antagonists, and W. circinata isolates were used as pathogens. Most isolates performed well in in vitro antagonistic assays, inhibiting by 54–59% the growth of seven plant pathogens: Bipolaris sorokiniana, Fusarium graminearum, Fusarium oxysporum, Pythium irregulare, two different isolates of W. circinata and Sclerotium rolfsii. The most inhibited pathogen was P. irregulare (72.6%), followed by S. rolfsii (66.7%) and W. circinata (~ 65%). There was no evidence of mycoparasitism. Their capacity for chitinase, cellulase, protease and ligninase (laccase, Mn-peroxidase and lignin-peroxidase) secretion was evaluated. Most of the isolates tested positive for chitinase activity. All the evaluated W. arvalis isolates produced cellulase, protease and laccase activities, and some of them also rendered positive results for Mn-peroxidase and decolorized the dye Azure B. Isolates INTA-L003 and INTA-L005 displayed top results for cellulase activity (both in carboxymethylcellulose plate assay and in filter paper tube assay). These activities might be responsible for their better antagonistic behavior on Pythium sp. Thus, these isolates are promising as biocontrol agents and as sources of new enzymes for industrial applications. Moreover, to the best of our knowledge, this is the first citation of W. arvalis in Argentina. |
| format | info:ar-repo/semantics/artículo |
| id | INTA18232 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA182322024-06-24T10:25:23Z Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi Melignani, Eliana Guidobono, Juan Santiago Rojo, Rodrigo Levin, Laura Barrera, Viviana Andrea Laetisaria Cellulose Biological Control Pythium Basidiomycota Plant Pathology Celulosa Control Biológico Fitopatología Waitea Ten Argentinean Waitea circinata-like isolates (Corticiales, Basidiomycota) obtained from soil samples were identified to species level based on morphological features and molecular tools, and tested in order to explore their biological control abilities. Eight of them were identified as Waitea arvalis and two as W. circinata. Waitea arvalis isolates were tested as antagonists, and W. circinata isolates were used as pathogens. Most isolates performed well in in vitro antagonistic assays, inhibiting by 54–59% the growth of seven plant pathogens: Bipolaris sorokiniana, Fusarium graminearum, Fusarium oxysporum, Pythium irregulare, two different isolates of W. circinata and Sclerotium rolfsii. The most inhibited pathogen was P. irregulare (72.6%), followed by S. rolfsii (66.7%) and W. circinata (~ 65%). There was no evidence of mycoparasitism. Their capacity for chitinase, cellulase, protease and ligninase (laccase, Mn-peroxidase and lignin-peroxidase) secretion was evaluated. Most of the isolates tested positive for chitinase activity. All the evaluated W. arvalis isolates produced cellulase, protease and laccase activities, and some of them also rendered positive results for Mn-peroxidase and decolorized the dye Azure B. Isolates INTA-L003 and INTA-L005 displayed top results for cellulase activity (both in carboxymethylcellulose plate assay and in filter paper tube assay). These activities might be responsible for their better antagonistic behavior on Pythium sp. Thus, these isolates are promising as biocontrol agents and as sources of new enzymes for industrial applications. Moreover, to the best of our knowledge, this is the first citation of W. arvalis in Argentina. Centro de Investigación en Ciencias Veterinarias y Agronómicas Fil: Melignani, Eliana. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Instituto de Micología y Botánica. Laboratorio de Micología Experimental; Argentina Fil: Melignani, Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Guidobono, Juan Santiago. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Ecología, Genética y Evolución. Instituto de Ecología y Genética de Buenos Aires. Laboratorio de Ecología de Poblaciones; Argentina Fil: Guidobono, Juan Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rojo, Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina Fil: Levin, Laura. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental. Instituto de Micología y Botánica. Laboratorio de Micología Experimental; Argentina Fil: Levin, Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola. Grupo Interacción Microorganismo-Planta; Argentina Fil: Barrera, Viviana Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Barrera, Viviana Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2024-06-24T10:17:56Z 2024-06-24T10:17:56Z 2024-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18232 https://link.springer.com/article/10.1007/s11557-024-01944-6 1617-416X https://doi.org/10.1007/s11557-024-01944-6 eng info:eu-repograntAgreement/INTA/2019-PD-E4-I069-001, Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetal info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Mycological Progress 23 : 16 (Published: 24 February 2024) |
| spellingShingle | Laetisaria Cellulose Biological Control Pythium Basidiomycota Plant Pathology Celulosa Control Biológico Fitopatología Waitea Melignani, Eliana Guidobono, Juan Santiago Rojo, Rodrigo Levin, Laura Barrera, Viviana Andrea Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi |
| title | Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi |
| title_full | Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi |
| title_fullStr | Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi |
| title_full_unstemmed | Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi |
| title_short | Studies on the biotechnological potential of Argentinean isolates of Waitea arvalis (Corticiales, Basidiomycota) for the biological control of phytopathogenic fungi |
| title_sort | studies on the biotechnological potential of argentinean isolates of waitea arvalis corticiales basidiomycota for the biological control of phytopathogenic fungi |
| topic | Laetisaria Cellulose Biological Control Pythium Basidiomycota Plant Pathology Celulosa Control Biológico Fitopatología Waitea |
| url | http://hdl.handle.net/20.500.12123/18232 https://link.springer.com/article/10.1007/s11557-024-01944-6 https://doi.org/10.1007/s11557-024-01944-6 |
| work_keys_str_mv | AT melignanieliana studiesonthebiotechnologicalpotentialofargentineanisolatesofwaiteaarvaliscorticialesbasidiomycotaforthebiologicalcontrolofphytopathogenicfungi AT guidobonojuansantiago studiesonthebiotechnologicalpotentialofargentineanisolatesofwaiteaarvaliscorticialesbasidiomycotaforthebiologicalcontrolofphytopathogenicfungi AT rojorodrigo studiesonthebiotechnologicalpotentialofargentineanisolatesofwaiteaarvaliscorticialesbasidiomycotaforthebiologicalcontrolofphytopathogenicfungi AT levinlaura studiesonthebiotechnologicalpotentialofargentineanisolatesofwaiteaarvaliscorticialesbasidiomycotaforthebiologicalcontrolofphytopathogenicfungi AT barreravivianaandrea studiesonthebiotechnologicalpotentialofargentineanisolatesofwaiteaarvaliscorticialesbasidiomycotaforthebiologicalcontrolofphytopathogenicfungi |