Salmonella spp. isolation from different types of egg samples and antimicrobial resistance of these strains in Argentina
We studied the serovars present and the performance of two selective plating media, Hektoen enteric agar (HE) and Xylose-Lysine-Desoxycholate (XLD) agar, as well as the time of tetrathionate broth incubation (one and five days) used for Salmonella isolation in different samples of egg and egg packs...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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GMPC
2024
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| Online Access: | http://hdl.handle.net/20.500.12123/18191 https://www.gmpc-akademie.de/articles/gjm/single/192 https://doi.org/10.51585/gjm.2024.2.0037 |
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| author | Soria, Mario Costa, Aldo Tomás Chichi, Matías Bruno, Susana B. Terragno, Raquel Tellez-Isaias, Guillermo Bueno, Dante Javier |
| author_browse | Bruno, Susana B. Bueno, Dante Javier Chichi, Matías Costa, Aldo Tomás Soria, Mario Tellez-Isaias, Guillermo Terragno, Raquel |
| author_facet | Soria, Mario Costa, Aldo Tomás Chichi, Matías Bruno, Susana B. Terragno, Raquel Tellez-Isaias, Guillermo Bueno, Dante Javier |
| author_sort | Soria, Mario |
| collection | INTA Digital |
| description | We studied the serovars present and the performance of two selective plating media, Hektoen enteric agar (HE) and Xylose-Lysine-Desoxycholate (XLD) agar, as well as the time of tetrathionate broth incubation (one and five days) used for Salmonella isolation in different samples of egg and egg packs collected from supermarkets in Argentina. We also determined the occurrence of bacterial growth inhibitors in egg content and the antibiotic resistance profile of the isolated strains. Salmonella spp. was detected in 29 samples (1.8%) from 1,643 different types of egg and egg pack samples analyzed. Eight serovars of Salmonella were isolated: S. ser. Typhimurium (ST), S. ser. Enteritidis (SE), S. ser. Agona, S. ser. Westhampton, S. ser. Brandenburg, S. ser. Gallinarum (biovar Gallinarum), S. ser. Muenchen, and S. ser. Montevideo. The most frequent were ST and SE; both were present in eggshells, yolk, and albumen. The increase in the incubation time in tetrathionate broth affected the isolation of Salmonella spp. from eggshell and pool of yolk and albumen with different values of relative sensitivity and accuracy (p<0.05). Furthermore, there was a significant difference between the two-plating media at the time of tetrathionate broth incubation in eggshell for the relative sensitivity (p<0.05). The agreement between XLD and HE agar in relation to the time of tetrathionate broth incubation depended on the type of samples studied. The occurrence of bacterial growth inhibitors in yolk and albumen depended on bacteria assayed. However, these were presented in yolk and albumen from the same pool of egg content. There were 2 and 8 different patterns of resistance among Salmonella strains isolated, and 87% of these strains were multidrug-resistant. It is necessary to understand the epidemiology of Salmonella from eggs and to alert public health to minimize the prevalence of antibiotic resistance of Salmonella spp. in Argentina. |
| format | info:ar-repo/semantics/artículo |
| id | INTA18191 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | GMPC |
| publisherStr | GMPC |
| record_format | dspace |
| spelling | INTA181912024-06-18T11:24:42Z Salmonella spp. isolation from different types of egg samples and antimicrobial resistance of these strains in Argentina Soria, Mario Costa, Aldo Tomás Chichi, Matías Bruno, Susana B. Terragno, Raquel Tellez-Isaias, Guillermo Bueno, Dante Javier Salmonella Huevos Resistencia a los Antibióticos Medio de Cultivo Antibióticos Argentina Eggs Resistance to Antibiotics Culture Media Antibiotics Bacterial Growth Inhibitor Inhibidores del crecimiento Bacteriano We studied the serovars present and the performance of two selective plating media, Hektoen enteric agar (HE) and Xylose-Lysine-Desoxycholate (XLD) agar, as well as the time of tetrathionate broth incubation (one and five days) used for Salmonella isolation in different samples of egg and egg packs collected from supermarkets in Argentina. We also determined the occurrence of bacterial growth inhibitors in egg content and the antibiotic resistance profile of the isolated strains. Salmonella spp. was detected in 29 samples (1.8%) from 1,643 different types of egg and egg pack samples analyzed. Eight serovars of Salmonella were isolated: S. ser. Typhimurium (ST), S. ser. Enteritidis (SE), S. ser. Agona, S. ser. Westhampton, S. ser. Brandenburg, S. ser. Gallinarum (biovar Gallinarum), S. ser. Muenchen, and S. ser. Montevideo. The most frequent were ST and SE; both were present in eggshells, yolk, and albumen. The increase in the incubation time in tetrathionate broth affected the isolation of Salmonella spp. from eggshell and pool of yolk and albumen with different values of relative sensitivity and accuracy (p<0.05). Furthermore, there was a significant difference between the two-plating media at the time of tetrathionate broth incubation in eggshell for the relative sensitivity (p<0.05). The agreement between XLD and HE agar in relation to the time of tetrathionate broth incubation depended on the type of samples studied. The occurrence of bacterial growth inhibitors in yolk and albumen depended on bacteria assayed. However, these were presented in yolk and albumen from the same pool of egg content. There were 2 and 8 different patterns of resistance among Salmonella strains isolated, and 87% of these strains were multidrug-resistant. It is necessary to understand the epidemiology of Salmonella from eggs and to alert public health to minimize the prevalence of antibiotic resistance of Salmonella spp. in Argentina. EEA Concepción del Uruguay Fil: Soria, Mario Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Costa, Aldo Tomás. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Salud; Argentina Fil: Chichi, Matías. Universidad Nacional de Entre Ríos. Facultad de Ciencias de la Salud; Argentina Fil: Bruno, Susana B. Administración Nacional de Laboratorios e Institutos de Salud (A.N.L.I.S.) ‘‘Dr Carlos G. Malbrán’’. Instituto Nacional de Producción de Biológicos (INPB). Servicio de Antígenos y Antisueros; Argentina Fil: Terragno, Raquel. Administración Nacional de Laboratorios e Institutos de Salud (A.N.L.I.S.) ‘‘Dr Carlos G. Malbrán’’. Instituto Nacional de Producción de Biológicos (INPB). Instituto Nacional de Enfermedades Infecciosas (I.N.E.I.). Servicio de Enterobacterias; Argentina Fil: Tellez-Isaias, Guillermo. University of Arkansas Agricultural Experiment Station. Department of Poultry Science; Estados Unidos Fil: Bueno, Dante Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay; Argentina Fil: Bueno, Dante Javier. Universidad Autónoma de Entre Ríos: Sede Basavilbaso. Facultad de Ciencia y Tecnología; Argentina 2024-06-18T10:32:55Z 2024-06-18T10:32:55Z 2024-06 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/18191 https://www.gmpc-akademie.de/articles/gjm/single/192 2749-0149 (Online) https://doi.org/10.51585/gjm.2024.2.0037 eng info:eu-repograntAgreement/INTA/2023-PE-L04-I120, Inocuidad física, química y biológica de los alimentos. Metodologías analíticas, tecnologías, estrategias de intervención innovadoras y análisis de riesgo info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Attribution-NonCommercial-4.0 International (CC BY-NC-4.0) application/pdf Argentina .......... (nation) (World, South America) 7006477 GMPC German journal of microbiology 4 (2): 54-66. (06-Jun-2024) |
| spellingShingle | Salmonella Huevos Resistencia a los Antibióticos Medio de Cultivo Antibióticos Argentina Eggs Resistance to Antibiotics Culture Media Antibiotics Bacterial Growth Inhibitor Inhibidores del crecimiento Bacteriano Soria, Mario Costa, Aldo Tomás Chichi, Matías Bruno, Susana B. Terragno, Raquel Tellez-Isaias, Guillermo Bueno, Dante Javier Salmonella spp. isolation from different types of egg samples and antimicrobial resistance of these strains in Argentina |
| title | Salmonella spp. isolation from different types of egg samples and antimicrobial resistance of these strains in Argentina |
| title_full | Salmonella spp. isolation from different types of egg samples and antimicrobial resistance of these strains in Argentina |
| title_fullStr | Salmonella spp. isolation from different types of egg samples and antimicrobial resistance of these strains in Argentina |
| title_full_unstemmed | Salmonella spp. isolation from different types of egg samples and antimicrobial resistance of these strains in Argentina |
| title_short | Salmonella spp. isolation from different types of egg samples and antimicrobial resistance of these strains in Argentina |
| title_sort | salmonella spp isolation from different types of egg samples and antimicrobial resistance of these strains in argentina |
| topic | Salmonella Huevos Resistencia a los Antibióticos Medio de Cultivo Antibióticos Argentina Eggs Resistance to Antibiotics Culture Media Antibiotics Bacterial Growth Inhibitor Inhibidores del crecimiento Bacteriano |
| url | http://hdl.handle.net/20.500.12123/18191 https://www.gmpc-akademie.de/articles/gjm/single/192 https://doi.org/10.51585/gjm.2024.2.0037 |
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