Impact of extracellular nucleic acids from lactic acid bacteria on qPCR and RT-qPCR results in dairy matrices : implications for defining molecular markers of cell integrity
In this study, we evaluated whether quantitative PCR (qPCR) and reverse transcription-qPCR (RT-qPCR) results could be affected by extracellular (free) DNA or RNA, respectively, obtained either from isolated bacteria or from fermented dairy foods manufactured with these microorganisms. These experime...
| Main Authors: | , , , , , , |
|---|---|
| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/1742 https://www.sciencedirect.com/science/article/pii/S002364381730155X https://doi.org/10.1016/j.lwt.2017.03.010 |
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