Improving the Comprehension of Pathogenicity and Phylogeny in ‘Candidatus Phytoplasma meliae’ through Genome Characterization
‘Candidatus Phytoplasma meliae’ is a pathogen associated with chinaberry yellowing disease, which has become a major phytosanitary problem for chinaberry forestry production in Argentina. Despite its economic impact, no genome information of this phytoplasma has been published, which has hindered...
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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MDPI
2024
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/17094 https://www.mdpi.com/2076-2607/12/1/142 https://doi.org/10.3390/microorganisms12010142 |
| _version_ | 1855037608535523328 |
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| author | Fernandez, Franco Daniel Xiao-Hua, Yan Chih-Horng, Kuo Marcone, Carmine Conci, Luis Rogelio |
| author_browse | Chih-Horng, Kuo Conci, Luis Rogelio Fernandez, Franco Daniel Marcone, Carmine Xiao-Hua, Yan |
| author_facet | Fernandez, Franco Daniel Xiao-Hua, Yan Chih-Horng, Kuo Marcone, Carmine Conci, Luis Rogelio |
| author_sort | Fernandez, Franco Daniel |
| collection | INTA Digital |
| description | ‘Candidatus Phytoplasma meliae’ is a pathogen associated with chinaberry yellowing
disease, which has become a major phytosanitary problem for chinaberry forestry production in
Argentina. Despite its economic impact, no genome information of this phytoplasma has been
published, which has hindered its characterization at the genomic level. In this study, we used a
metagenomics approach to analyze the draft genome of the ‘Ca. P. meliae’ strain ChTYXIII. The draft
assembly consisted of twenty-one contigs with a total length of 751.949 bp, and annotation revealed
669 CDSs, 34 tRNAs, and 1 set of rRNA operons. The metabolic pathways analysis showed that
ChTYXIII contains the complete core genes for glycolysis and a functional Sec system for protein
translocation. Our phylogenomic analysis based on 133 single-copy genes and genome-to-genome
metrics supports the classification as unique ‘Ca. P. species’ within the MPV clade. |
| format | info:ar-repo/semantics/artículo |
| id | INTA17094 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INTA170942024-03-19T11:22:01Z Improving the Comprehension of Pathogenicity and Phylogeny in ‘Candidatus Phytoplasma meliae’ through Genome Characterization Fernandez, Franco Daniel Xiao-Hua, Yan Chih-Horng, Kuo Marcone, Carmine Conci, Luis Rogelio Phytoplasmas Genomes Fitoplasmas Genomas Melia azedarach Syntheny Effector Protein Chinaberry Orthologs MPV Phytoplasma meliae ‘Candidatus Phytoplasma meliae’ is a pathogen associated with chinaberry yellowing disease, which has become a major phytosanitary problem for chinaberry forestry production in Argentina. Despite its economic impact, no genome information of this phytoplasma has been published, which has hindered its characterization at the genomic level. In this study, we used a metagenomics approach to analyze the draft genome of the ‘Ca. P. meliae’ strain ChTYXIII. The draft assembly consisted of twenty-one contigs with a total length of 751.949 bp, and annotation revealed 669 CDSs, 34 tRNAs, and 1 set of rRNA operons. The metabolic pathways analysis showed that ChTYXIII contains the complete core genes for glycolysis and a functional Sec system for protein translocation. Our phylogenomic analysis based on 133 single-copy genes and genome-to-genome metrics supports the classification as unique ‘Ca. P. species’ within the MPV clade. Instituto de Patología Vegetal Fil: Fernandez, Franco Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Fernandez, Franco Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Xiao-Hua, Yan. Academia Sinica. Institute of Plant and Microbial Biology; Taiwan Fil: Chih-Horng, Kuo. Academia Sinica. Institute of Plant and Microbial Biology; Taiwan Fil: Marcone, Carmine. University of Salerno. Department of Pharmacy; Italia Fil: Conci, Luis Rogelio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Conci, Luis Rogelio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina 2024-03-19T11:13:01Z 2024-03-19T11:13:01Z 2024-01-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/17094 https://www.mdpi.com/2076-2607/12/1/142 2076-2607 https://doi.org/10.3390/microorganisms12010142 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf MDPI Microorganisms 12 (1) : 142 (January 2024) |
| spellingShingle | Phytoplasmas Genomes Fitoplasmas Genomas Melia azedarach Syntheny Effector Protein Chinaberry Orthologs MPV Phytoplasma meliae Fernandez, Franco Daniel Xiao-Hua, Yan Chih-Horng, Kuo Marcone, Carmine Conci, Luis Rogelio Improving the Comprehension of Pathogenicity and Phylogeny in ‘Candidatus Phytoplasma meliae’ through Genome Characterization |
| title | Improving the Comprehension of Pathogenicity and Phylogeny in ‘Candidatus Phytoplasma meliae’ through Genome Characterization |
| title_full | Improving the Comprehension of Pathogenicity and Phylogeny in ‘Candidatus Phytoplasma meliae’ through Genome Characterization |
| title_fullStr | Improving the Comprehension of Pathogenicity and Phylogeny in ‘Candidatus Phytoplasma meliae’ through Genome Characterization |
| title_full_unstemmed | Improving the Comprehension of Pathogenicity and Phylogeny in ‘Candidatus Phytoplasma meliae’ through Genome Characterization |
| title_short | Improving the Comprehension of Pathogenicity and Phylogeny in ‘Candidatus Phytoplasma meliae’ through Genome Characterization |
| title_sort | improving the comprehension of pathogenicity and phylogeny in candidatus phytoplasma meliae through genome characterization |
| topic | Phytoplasmas Genomes Fitoplasmas Genomas Melia azedarach Syntheny Effector Protein Chinaberry Orthologs MPV Phytoplasma meliae |
| url | http://hdl.handle.net/20.500.12123/17094 https://www.mdpi.com/2076-2607/12/1/142 https://doi.org/10.3390/microorganisms12010142 |
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