Prevalence, risk factors and molecular epidemiology of neonatal cryptosporidiosis of calves : The Argentine perspective
Cryptosporidium spp. are enteroparasitic protozoans that cause cryptosporidiosis in newborn calves. Clinical signs of the infection are diarrhoea and dehydration leading to decreased productivity and economic losses in cattle farms around the world. Additionally, cryptosporidiosis is a relevant zoon...
| Autores principales: | , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/16119 https://www.sciencedirect.com/science/article/pii/S2667114X23000353 https://doi.org/10.1016/j.crpvbd.2023.100147 |
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| author | De Alba, Paloma Garro, Carlos Javier Florin-Christensen, Monica Schnittger, Leonhard |
| author_browse | De Alba, Paloma Florin-Christensen, Monica Garro, Carlos Javier Schnittger, Leonhard |
| author_facet | De Alba, Paloma Garro, Carlos Javier Florin-Christensen, Monica Schnittger, Leonhard |
| author_sort | De Alba, Paloma |
| collection | INTA Digital |
| description | Cryptosporidium spp. are enteroparasitic protozoans that cause cryptosporidiosis in newborn calves. Clinical signs of the infection are diarrhoea and dehydration leading to decreased productivity and economic losses in cattle farms around the world. Additionally, cryptosporidiosis is a relevant zoonotic disease since the ingestion of oocysts can be fatal for children under five years of age, the elderly, and/or immunocompromised adults. This review aims to integrate existing knowledge on the epidemiological situation of calf cryptosporidiosis and associated risk factors in Argentina. In addition, the GP60 subtype diversity of the pathogen was analysed and related with the global distribution of corresponding GP60 subtypes. Depending on the study region and applied diagnostics, prevalence among calves up to 20 days of age varied between 25.2% and 42.5%, while a prevalence of 16.3–25.5% was observed at the age of 1–90 days. So far, molecular studies have determined exclusively Cryptosporidium parvum in preweaned calves. In addition, C. parvum infection was reported as the major cause of calf diarrhoea, followed by rotavirus A (RVA), while enteropathogens such as coronavirus, Escherichia coli, and Salmonella sp. played a negligible role. Calf age of 20 days or less, incidence of diarrhoea, poorly drained soils, and large farm size were identified as risk factors for C. parvum-infection in Argentina. A total of nine GP60 subtypes (IIaAxxG1R1, xx = 16 to 24) were identified, showing a stepwise increase of the trinucleotide motif TCA, and including the zoonotic subtypes IIaA16G1R1, IIaA17G1R1, IIaA18G1R1, IIaA19G1R1, and IIaA20G1R1. We found that an increase in the A16→A24 trinucleotide repeat was accompanied by a gradual decrease in the global distribution of GP60 alleles, strongly suggesting that IIaA16G1R1 represents the primordial allelic variant of this group. Since identified GP60 alleles have a similar genetic background, we hypothesize that the continuous trinucleotide repeat array has been generated by stepwise repeat expansion of A16. The information gathered and integrated in this study contributes to an improved understanding of the epidemiological characteristics of bovine cryptosporidiosis in and beyond Argentina, which in turn can help to develop control strategies for this parasitosis of veterinary and medical relevance. |
| format | info:ar-repo/semantics/artículo |
| id | INTA16119 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA161192023-12-05T10:22:47Z Prevalence, risk factors and molecular epidemiology of neonatal cryptosporidiosis of calves : The Argentine perspective De Alba, Paloma Garro, Carlos Javier Florin-Christensen, Monica Schnittger, Leonhard Cryptosporidium parvum Ternero Diarrea Epidemiología Factores de Riesgo Argentina Calves Diarrhoea Epidemiology Risk Factors Neonatal Diarrhoea Prevalence Diarrea Neonatal Predominio Cryptosporidium spp. are enteroparasitic protozoans that cause cryptosporidiosis in newborn calves. Clinical signs of the infection are diarrhoea and dehydration leading to decreased productivity and economic losses in cattle farms around the world. Additionally, cryptosporidiosis is a relevant zoonotic disease since the ingestion of oocysts can be fatal for children under five years of age, the elderly, and/or immunocompromised adults. This review aims to integrate existing knowledge on the epidemiological situation of calf cryptosporidiosis and associated risk factors in Argentina. In addition, the GP60 subtype diversity of the pathogen was analysed and related with the global distribution of corresponding GP60 subtypes. Depending on the study region and applied diagnostics, prevalence among calves up to 20 days of age varied between 25.2% and 42.5%, while a prevalence of 16.3–25.5% was observed at the age of 1–90 days. So far, molecular studies have determined exclusively Cryptosporidium parvum in preweaned calves. In addition, C. parvum infection was reported as the major cause of calf diarrhoea, followed by rotavirus A (RVA), while enteropathogens such as coronavirus, Escherichia coli, and Salmonella sp. played a negligible role. Calf age of 20 days or less, incidence of diarrhoea, poorly drained soils, and large farm size were identified as risk factors for C. parvum-infection in Argentina. A total of nine GP60 subtypes (IIaAxxG1R1, xx = 16 to 24) were identified, showing a stepwise increase of the trinucleotide motif TCA, and including the zoonotic subtypes IIaA16G1R1, IIaA17G1R1, IIaA18G1R1, IIaA19G1R1, and IIaA20G1R1. We found that an increase in the A16→A24 trinucleotide repeat was accompanied by a gradual decrease in the global distribution of GP60 alleles, strongly suggesting that IIaA16G1R1 represents the primordial allelic variant of this group. Since identified GP60 alleles have a similar genetic background, we hypothesize that the continuous trinucleotide repeat array has been generated by stepwise repeat expansion of A16. The information gathered and integrated in this study contributes to an improved understanding of the epidemiological characteristics of bovine cryptosporidiosis in and beyond Argentina, which in turn can help to develop control strategies for this parasitosis of veterinary and medical relevance. Instituto de Patobiología Fil: De Alba, Paloma. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: De Alba, Paloma. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Garro, Carlos Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Florin-Christensen, Monica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Florin-Christensen, Monica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Schnittger, Leonhard. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-12-05T10:15:28Z 2023-12-05T10:15:28Z 2023-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/16119 https://www.sciencedirect.com/science/article/pii/S2667114X23000353 2667-114X https://doi.org/10.1016/j.crpvbd.2023.100147 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I102-001, Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública info:eu-repograntAgreement/INTA/2019-PD-E5-I103-001, Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud pública info:eu-repograntAgreement/INTA/2023-PD-L01-I118, Innovaciones en los procesos de crianza, recría y vaca en transición de rodeos lecheros info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Current Research in Parasitology & Vector-Borne Diseases 4 : 100147 (Available online 4 October 2023) |
| spellingShingle | Cryptosporidium parvum Ternero Diarrea Epidemiología Factores de Riesgo Argentina Calves Diarrhoea Epidemiology Risk Factors Neonatal Diarrhoea Prevalence Diarrea Neonatal Predominio De Alba, Paloma Garro, Carlos Javier Florin-Christensen, Monica Schnittger, Leonhard Prevalence, risk factors and molecular epidemiology of neonatal cryptosporidiosis of calves : The Argentine perspective |
| title | Prevalence, risk factors and molecular epidemiology of neonatal cryptosporidiosis of calves : The Argentine perspective |
| title_full | Prevalence, risk factors and molecular epidemiology of neonatal cryptosporidiosis of calves : The Argentine perspective |
| title_fullStr | Prevalence, risk factors and molecular epidemiology of neonatal cryptosporidiosis of calves : The Argentine perspective |
| title_full_unstemmed | Prevalence, risk factors and molecular epidemiology of neonatal cryptosporidiosis of calves : The Argentine perspective |
| title_short | Prevalence, risk factors and molecular epidemiology of neonatal cryptosporidiosis of calves : The Argentine perspective |
| title_sort | prevalence risk factors and molecular epidemiology of neonatal cryptosporidiosis of calves the argentine perspective |
| topic | Cryptosporidium parvum Ternero Diarrea Epidemiología Factores de Riesgo Argentina Calves Diarrhoea Epidemiology Risk Factors Neonatal Diarrhoea Prevalence Diarrea Neonatal Predominio |
| url | http://hdl.handle.net/20.500.12123/16119 https://www.sciencedirect.com/science/article/pii/S2667114X23000353 https://doi.org/10.1016/j.crpvbd.2023.100147 |
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