Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves
The use of synthetic fungicides for control soybean crop diseases has been cuestionated because of its impacts on human health and the environment. This work evaluated the composition, antifungal activity and toxicity of the alkaloids fraction obtained from Neltuma nigra leaves and compared its effe...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/15939 https://www.sciencedirect.com/science/article/pii/S1878818123003158 https://doi.org/10.1016/j.bcab.2023.102914 |
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| author | Sequin, Christian J. Appelhans, Stefania Carolina Heis, Magalí S. Torrent, Walter A. Trossero, Julia A. Catalan, Cesar Atilio Nazareno Sampietro, Diego Alejandro Aceñolaza, Pablo Gilberto |
| author_browse | Aceñolaza, Pablo Gilberto Appelhans, Stefania Carolina Catalan, Cesar Atilio Nazareno Heis, Magalí S. Sampietro, Diego Alejandro Sequin, Christian J. Torrent, Walter A. Trossero, Julia A. |
| author_facet | Sequin, Christian J. Appelhans, Stefania Carolina Heis, Magalí S. Torrent, Walter A. Trossero, Julia A. Catalan, Cesar Atilio Nazareno Sampietro, Diego Alejandro Aceñolaza, Pablo Gilberto |
| author_sort | Sequin, Christian J. |
| collection | INTA Digital |
| description | The use of synthetic fungicides for control soybean crop diseases has been cuestionated because of its impacts on human health and the environment. This work evaluated the composition, antifungal activity and toxicity of the alkaloids fraction obtained from Neltuma nigra leaves and compared its effect with the fungicide difenoconazole on Sclerotium rolfsii, Cercospora kikuchii, and C. sojina. Alkaloids fraction was extracted from leaves of N. nigra and its composition was analyzed by gas-chromatography coupled to mass spectrometry (GC-MS). The antifungal effect was recorded by measuring diameters of halos of inhibition of the growth of the different evaluated fungis. The toxicity of the alkaloids fraction was carried out using the method of Caenorhabditis elegans toxicity test. The extracted alkaloids fraction contained the alkaloids tryptamine (63.9%), tetrahydroxyharman (13.8%), phenethylamine (11.1%), cassine (10.4%), and N-formylphenylethylamine (0.8%). The alkaloids fraction was as effective against S. rolfsii as on the Cercospora strains, with minimum inhibitory doses of 25.0 μg disc-1 and inhibitory halos in the range of 8.5-13.0 mm, while difenoconazole had no effect on S. rolfsii. The toxicity of the alkaloid fraction (30.4-68.8 mg L-1) and difenoconazole (0.1-2.8 mg L-1) was tested on the reproduction, body length, and fertility of C. elegans. The fraction reduced the reproductive rate by 28.8-72.4% whereas difenoconazole did it from 12.2% to complete suppression. Neither of them affected the fertility of C. elegans. These findings suggest that the alkaloids fraction of N. nigra leaves is less toxic than difenoconazole and is a promising botanical fungicide against pathogenic soybean fungi. |
| format | info:ar-repo/semantics/artículo |
| id | INTA15939 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA159392023-11-13T14:15:33Z Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves Sequin, Christian J. Appelhans, Stefania Carolina Heis, Magalí S. Torrent, Walter A. Trossero, Julia A. Catalan, Cesar Atilio Nazareno Sampietro, Diego Alejandro Aceñolaza, Pablo Gilberto Antifungal Plants Antifungal Properties Alkaloids Toxicology Propiedades Antimicosicas Alcaloides Fabaceae Toxicología Neltuma nigra The use of synthetic fungicides for control soybean crop diseases has been cuestionated because of its impacts on human health and the environment. This work evaluated the composition, antifungal activity and toxicity of the alkaloids fraction obtained from Neltuma nigra leaves and compared its effect with the fungicide difenoconazole on Sclerotium rolfsii, Cercospora kikuchii, and C. sojina. Alkaloids fraction was extracted from leaves of N. nigra and its composition was analyzed by gas-chromatography coupled to mass spectrometry (GC-MS). The antifungal effect was recorded by measuring diameters of halos of inhibition of the growth of the different evaluated fungis. The toxicity of the alkaloids fraction was carried out using the method of Caenorhabditis elegans toxicity test. The extracted alkaloids fraction contained the alkaloids tryptamine (63.9%), tetrahydroxyharman (13.8%), phenethylamine (11.1%), cassine (10.4%), and N-formylphenylethylamine (0.8%). The alkaloids fraction was as effective against S. rolfsii as on the Cercospora strains, with minimum inhibitory doses of 25.0 μg disc-1 and inhibitory halos in the range of 8.5-13.0 mm, while difenoconazole had no effect on S. rolfsii. The toxicity of the alkaloid fraction (30.4-68.8 mg L-1) and difenoconazole (0.1-2.8 mg L-1) was tested on the reproduction, body length, and fertility of C. elegans. The fraction reduced the reproductive rate by 28.8-72.4% whereas difenoconazole did it from 12.2% to complete suppression. Neither of them affected the fertility of C. elegans. These findings suggest that the alkaloids fraction of N. nigra leaves is less toxic than difenoconazole and is a promising botanical fungicide against pathogenic soybean fungi. EEA Paraná Fil: Sequin, Christian J. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina Fil: Appelhans, Estefania Carolina. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina Fil: Appelhans, Estefania Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; Argentina Fil: Heis, Magalí S. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina Fil: Torrent, Walter A. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina Fil: Trossero, Julia A. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina Fil: Catalan, Cesar Atilio Nazareno. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. LABIFITO; Argentina Fil: Sampietro, Diego Alejandro. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. LABIFITO; Argentina Fil: Aceñolaza, Pablo Gilberto. Universidad Nacional de Entre Ríos. Facultad de Agronomía; Argentina 2023-11-13T14:12:25Z 2023-11-13T14:12:25Z 2023-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15939 https://www.sciencedirect.com/science/article/pii/S1878818123003158 1878-8181 https://doi.org/10.1016/j.bcab.2023.102914 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Biocatalysis and Agricultural Biotechnology 54 : 102914. (November 2023) |
| spellingShingle | Antifungal Plants Antifungal Properties Alkaloids Toxicology Propiedades Antimicosicas Alcaloides Fabaceae Toxicología Neltuma nigra Sequin, Christian J. Appelhans, Stefania Carolina Heis, Magalí S. Torrent, Walter A. Trossero, Julia A. Catalan, Cesar Atilio Nazareno Sampietro, Diego Alejandro Aceñolaza, Pablo Gilberto Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves |
| title | Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves |
| title_full | Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves |
| title_fullStr | Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves |
| title_full_unstemmed | Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves |
| title_short | Antifungal and toxicological evaluation of the alkaloids fraction from Neltuma nigra leaves |
| title_sort | antifungal and toxicological evaluation of the alkaloids fraction from neltuma nigra leaves |
| topic | Antifungal Plants Antifungal Properties Alkaloids Toxicology Propiedades Antimicosicas Alcaloides Fabaceae Toxicología Neltuma nigra |
| url | http://hdl.handle.net/20.500.12123/15939 https://www.sciencedirect.com/science/article/pii/S1878818123003158 https://doi.org/10.1016/j.bcab.2023.102914 |
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