Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions
High-throughput sequencing of genomes has expanded our knowledge of the Alphaherpesvirinae, a widely extended subfamily of DNA viruses that recombine to increase their genetic diversity. It has been acknowledged that Equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4), two alphaherpesviruses...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
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Elsevier
2023
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/15284 https://doi.org/10.1016/j.jevs.2023.104916 |
| _version_ | 1855037284375592960 |
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| author | Tau, Rocio Lucia Ferreccio, Carola Maria Bachir, Natalia Veronica Torales, Fatima Anabel Romera, Sonia Alejandra Maidana, Silvina Soledad |
| author_browse | Bachir, Natalia Veronica Ferreccio, Carola Maria Maidana, Silvina Soledad Romera, Sonia Alejandra Tau, Rocio Lucia Torales, Fatima Anabel |
| author_facet | Tau, Rocio Lucia Ferreccio, Carola Maria Bachir, Natalia Veronica Torales, Fatima Anabel Romera, Sonia Alejandra Maidana, Silvina Soledad |
| author_sort | Tau, Rocio Lucia |
| collection | INTA Digital |
| description | High-throughput sequencing of genomes has expanded our knowledge of the Alphaherpesvirinae, a widely extended subfamily of DNA viruses that recombine to increase their genetic diversity. It has been acknowledged that Equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4), two alphaherpesviruses with an economic impact on the horse industry, can recombine. This work aimed to analyze interspecific recombination between all equid alphaherpesvirus species, using genomes of EHV-1, EHV-3, EHV-4, EHV-6, EHV-8, and EHV-9 available in GenBank. 14 events of recombination by RDP4 and Simplot between EHV-1 x EHV-4, EHV-1 x EHV-9, EHV-8 x EHV-1, and EHV-8 x EHV-9 were identified. 10 out of 14 events involved ORF64, a double-copy gene located at the repeat regions that codifies for the infected cell protein 4 (ICP4). Among the ICP4, recombination can be found between EHV-1 X EHV-9, EHV-8 X EHV-9, and EHV-1 X EHV-4, the former affects zebra-borne genotypes, a type of EHV-1 that infect wild equids, and the latter match with previous breakpoints reported in fields isolates. Consequently, these findings strongly suggest that ICP4 is a hotspot for recombination. This work describes novel recombination events and is the first genome-wide recombination analysis using all available equid alphaherpesvirus species genomes. |
| format | info:ar-repo/semantics/artículo |
| id | INTA15284 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA152842023-09-21T17:56:23Z Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions Tau, Rocio Lucia Ferreccio, Carola Maria Bachir, Natalia Veronica Torales, Fatima Anabel Romera, Sonia Alejandra Maidana, Silvina Soledad Herpesviridae Recombinación Homóloga Equidae Homologous Recombination Alphaherpesvirus High-throughput sequencing of genomes has expanded our knowledge of the Alphaherpesvirinae, a widely extended subfamily of DNA viruses that recombine to increase their genetic diversity. It has been acknowledged that Equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4), two alphaherpesviruses with an economic impact on the horse industry, can recombine. This work aimed to analyze interspecific recombination between all equid alphaherpesvirus species, using genomes of EHV-1, EHV-3, EHV-4, EHV-6, EHV-8, and EHV-9 available in GenBank. 14 events of recombination by RDP4 and Simplot between EHV-1 x EHV-4, EHV-1 x EHV-9, EHV-8 x EHV-1, and EHV-8 x EHV-9 were identified. 10 out of 14 events involved ORF64, a double-copy gene located at the repeat regions that codifies for the infected cell protein 4 (ICP4). Among the ICP4, recombination can be found between EHV-1 X EHV-9, EHV-8 X EHV-9, and EHV-1 X EHV-4, the former affects zebra-borne genotypes, a type of EHV-1 that infect wild equids, and the latter match with previous breakpoints reported in fields isolates. Consequently, these findings strongly suggest that ICP4 is a hotspot for recombination. This work describes novel recombination events and is the first genome-wide recombination analysis using all available equid alphaherpesvirus species genomes. Instituto de Virología Fil: Tau, Rocio Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Tau, Rocio Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ferrecio, Carola Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación en Virología; Argentina Fil: Ferrecio, Carola Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina Fil: Ferrecio, Carola Maria. Universidad del Salvador. Cátedra de Inmunología; Argentina Fil: Bachir, Natalia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Bachir, Natalia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Torales, Fatima Anabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Torales, Fatima Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Romera, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romera, Sonia Alejandra. Universidad del Salvador. Cátedra de Inmunología; Argentina Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina Fil: Maidana, Silvina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Maidana, Silvina Soledad. Universidad del Salvador. Cátedra de Inmunología; Argentina 2023-09-21T16:40:21Z 2023-09-21T16:40:21Z 2023 info:ar-repo/semantics/artículo info:eu-repo/semantics/acceptedVersion info:eu-repo/semantics/article http://hdl.handle.net/20.500.12123/15284 0737-0806 https://doi.org/10.1016/j.jevs.2023.104916 eng info:eu-repo/semantics/embargoedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Journal of equine veterinary science (Available online 11 September 2023) |
| spellingShingle | Herpesviridae Recombinación Homóloga Equidae Homologous Recombination Alphaherpesvirus Tau, Rocio Lucia Ferreccio, Carola Maria Bachir, Natalia Veronica Torales, Fatima Anabel Romera, Sonia Alejandra Maidana, Silvina Soledad Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions |
| title | Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions |
| title_full | Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions |
| title_fullStr | Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions |
| title_full_unstemmed | Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions |
| title_short | Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions |
| title_sort | comprehensive analysis of equid herpesvirus recombination an insight into the repeat regions |
| topic | Herpesviridae Recombinación Homóloga Equidae Homologous Recombination Alphaherpesvirus |
| url | http://hdl.handle.net/20.500.12123/15284 https://doi.org/10.1016/j.jevs.2023.104916 |
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