Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2
The accurate and effective detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to preventing the spread of infectious diseases and ensuring human health. Herein, a nanobody-displayed whole-cell biosensor was developed for colorimetric detection of SARS-CoV-2 spike...
| Autores principales: | , , , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
American Chemical Society
2023
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/15222 https://pubs.acs.org/doi/10.1021/acsami.3c05900 https://doi.org/10.1021/acsami.3c05900 |
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| author | He, Yawen Xu, Zhiyuan Kasputis, Tom Zhao, Xue Ibañez, Itati Pavan, Florencia Bok, Marina Malito, Juan Pablo Parreño, Gladys Viviana Yuan, Lijuan Wright, R. Clay Chen, Juhong |
| author_browse | Bok, Marina Chen, Juhong He, Yawen Ibañez, Itati Kasputis, Tom Malito, Juan Pablo Parreño, Gladys Viviana Pavan, Florencia Wright, R. Clay Xu, Zhiyuan Yuan, Lijuan Zhao, Xue |
| author_facet | He, Yawen Xu, Zhiyuan Kasputis, Tom Zhao, Xue Ibañez, Itati Pavan, Florencia Bok, Marina Malito, Juan Pablo Parreño, Gladys Viviana Yuan, Lijuan Wright, R. Clay Chen, Juhong |
| author_sort | He, Yawen |
| collection | INTA Digital |
| description | The accurate and effective detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to preventing the spread of infectious diseases and ensuring human health. Herein, a nanobody-displayed whole-cell biosensor was developed for colorimetric detection of SARS-CoV-2 spike proteins. Serving as bioreceptors, yeast surfaces were genetically engineered to display SARS-CoV-2 binding of llama-derived single-domain antibodies (nanobodies) with high capture efficiency, facilitating the concentration and purification of SARS-CoV-2. Gold nanoparticles (AuNPs) employed as signal transductions were functionalized with horseradish peroxidase (HRP) and anti-SARS monoclonal antibodies to enhance the detection sensitivity. In the presence of SARS-CoV-2 spike proteins, the sandwiched binding will be formed by linking engineered yeast, SARS-CoV-2 spike proteins, and reporter AuNPs. The colorimetric signal was generated by the enzymatic reaction of HRP and its corresponding colorimetric substrate/chromogen system. At the optimal conditions, the developed whole-cell biosensor enables the sensitive detection of SARS-CoV-2 spike proteins in a linear range from 0.01 to 1 μg/mL with a limit of detection (LOD) of 0.037 μg/mL (about 4 × 108 virion particles/mL). Furthermore, the whole-cell biosensor was demonstrated to detect the spike protein of different SARS-CoV-2 variants in human serum, providing new possibilities for the detection of future SARS-CoV-2 variants. |
| format | Artículo |
| id | INTA15222 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | American Chemical Society |
| publisherStr | American Chemical Society |
| record_format | dspace |
| spelling | INTA152222023-09-15T11:01:30Z Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2 He, Yawen Xu, Zhiyuan Kasputis, Tom Zhao, Xue Ibañez, Itati Pavan, Florencia Bok, Marina Malito, Juan Pablo Parreño, Gladys Viviana Yuan, Lijuan Wright, R. Clay Chen, Juhong Biosensors Colorimetry Severe Acute Respiratory Syndrome Coronavirus 2 Biosensores Colorimetría Coronavirus del Síndrome Respiratorio Agudo Grave 2 Nanobody Nanocuerpo SARS-CoV-2 The accurate and effective detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to preventing the spread of infectious diseases and ensuring human health. Herein, a nanobody-displayed whole-cell biosensor was developed for colorimetric detection of SARS-CoV-2 spike proteins. Serving as bioreceptors, yeast surfaces were genetically engineered to display SARS-CoV-2 binding of llama-derived single-domain antibodies (nanobodies) with high capture efficiency, facilitating the concentration and purification of SARS-CoV-2. Gold nanoparticles (AuNPs) employed as signal transductions were functionalized with horseradish peroxidase (HRP) and anti-SARS monoclonal antibodies to enhance the detection sensitivity. In the presence of SARS-CoV-2 spike proteins, the sandwiched binding will be formed by linking engineered yeast, SARS-CoV-2 spike proteins, and reporter AuNPs. The colorimetric signal was generated by the enzymatic reaction of HRP and its corresponding colorimetric substrate/chromogen system. At the optimal conditions, the developed whole-cell biosensor enables the sensitive detection of SARS-CoV-2 spike proteins in a linear range from 0.01 to 1 μg/mL with a limit of detection (LOD) of 0.037 μg/mL (about 4 × 108 virion particles/mL). Furthermore, the whole-cell biosensor was demonstrated to detect the spike protein of different SARS-CoV-2 variants in human serum, providing new possibilities for the detection of future SARS-CoV-2 variants. Instituto de Virología Fil: He, Yawen. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos Fil: Xu, Zhiyuan. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos Fil: Kasputis, Tom. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos Fil: Zhao, Xue. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos Fil: Ibañez, Itati. Universidad de Buenos Aires. Instituto de Química Física de los Materiales, Medio Ambiente y Energía (INQUIMAE); Argentina Fil: Ibañez, Itati. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pavan, Florencia. Universidad de Buenos Aires. Instituto de Química Física de los Materiales, Medio Ambiente y Energía (INQUIMAE); Argentina Fil: Pavan, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas; Argentina Fil: Bok, Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Malito, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virología e Innovaciones Tecnologicas; Argentina Fil: Malito, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). INCUINTA. Instituto de Virologia e Innovaciones Tecnologicas (IVIT); Argentina Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Parreño, Gladys Viviana. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Yuan, Lijuan. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Wright, R. Clay. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos Fil: Chen, Juhong. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos 2023-09-15T10:55:41Z 2023-09-15T10:55:41Z 2023-08 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15222 https://pubs.acs.org/doi/10.1021/acsami.3c05900 1944-8252 https://doi.org/10.1021/acsami.3c05900 eng info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf American Chemical Society ACS Applied Materials & Interfaces 15 : 37184-37192 (Agosto 2023) |
| spellingShingle | Biosensors Colorimetry Severe Acute Respiratory Syndrome Coronavirus 2 Biosensores Colorimetría Coronavirus del Síndrome Respiratorio Agudo Grave 2 Nanobody Nanocuerpo SARS-CoV-2 He, Yawen Xu, Zhiyuan Kasputis, Tom Zhao, Xue Ibañez, Itati Pavan, Florencia Bok, Marina Malito, Juan Pablo Parreño, Gladys Viviana Yuan, Lijuan Wright, R. Clay Chen, Juhong Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2 |
| title | Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2 |
| title_full | Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2 |
| title_fullStr | Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2 |
| title_full_unstemmed | Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2 |
| title_short | Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2 |
| title_sort | development of nanobody displayed whole cell biosensors for the colorimetric detection of sars cov 2 |
| topic | Biosensors Colorimetry Severe Acute Respiratory Syndrome Coronavirus 2 Biosensores Colorimetría Coronavirus del Síndrome Respiratorio Agudo Grave 2 Nanobody Nanocuerpo SARS-CoV-2 |
| url | http://hdl.handle.net/20.500.12123/15222 https://pubs.acs.org/doi/10.1021/acsami.3c05900 https://doi.org/10.1021/acsami.3c05900 |
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