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Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2

The accurate and effective detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to preventing the spread of infectious diseases and ensuring human health. Herein, a nanobody-displayed whole-cell biosensor was developed for colorimetric detection of SARS-CoV-2 spike...

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Autores principales: He, Yawen, Xu, Zhiyuan, Kasputis, Tom, Zhao, Xue, Ibañez, Itati, Pavan, Florencia, Bok, Marina, Malito, Juan Pablo, Parreño, Gladys Viviana, Yuan, Lijuan, Wright, R. Clay, Chen, Juhong
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: American Chemical Society 2023
Materias:
Biosensors
Colorimetry
Severe Acute Respiratory Syndrome Coronavirus 2
Biosensores
Colorimetría
Coronavirus del Síndrome Respiratorio Agudo Grave 2
Nanobody
Nanocuerpo
SARS-CoV-2
Acceso en línea:http://hdl.handle.net/20.500.12123/15222
https://pubs.acs.org/doi/10.1021/acsami.3c05900
https://doi.org/10.1021/acsami.3c05900
Sumario:The accurate and effective detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to preventing the spread of infectious diseases and ensuring human health. Herein, a nanobody-displayed whole-cell biosensor was developed for colorimetric detection of SARS-CoV-2 spike proteins. Serving as bioreceptors, yeast surfaces were genetically engineered to display SARS-CoV-2 binding of llama-derived single-domain antibodies (nanobodies) with high capture efficiency, facilitating the concentration and purification of SARS-CoV-2. Gold nanoparticles (AuNPs) employed as signal transductions were functionalized with horseradish peroxidase (HRP) and anti-SARS monoclonal antibodies to enhance the detection sensitivity. In the presence of SARS-CoV-2 spike proteins, the sandwiched binding will be formed by linking engineered yeast, SARS-CoV-2 spike proteins, and reporter AuNPs. The colorimetric signal was generated by the enzymatic reaction of HRP and its corresponding colorimetric substrate/chromogen system. At the optimal conditions, the developed whole-cell biosensor enables the sensitive detection of SARS-CoV-2 spike proteins in a linear range from 0.01 to 1 μg/mL with a limit of detection (LOD) of 0.037 μg/mL (about 4 × 108 virion particles/mL). Furthermore, the whole-cell biosensor was demonstrated to detect the spike protein of different SARS-CoV-2 variants in human serum, providing new possibilities for the detection of future SARS-CoV-2 variants.

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