Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype
In a previous study, we evaluated the degree of virulence of Mycobacterium avium subsp. paratuberculosis (Map) strains isolated from cattle in Argentina in a murine model. This assay allowed us to differentiate between high-virulent MapARG1347 and low-virulent MapARG1543 strains. To corroborate whet...
| Autores principales: | , , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2023
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/15156 https://www.sciencedirect.com/science/article/pii/S1472979222001366 https://doi.org/10.1016/j.tube.2022.102299 |
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| author | Colombatti Olivieri, Maria Alejandra Fresia, Pablo Graña, Martín Cuerda, Maria Ximena Nagel, Ariel Gaston Alvarado Pinedo, Maria Fiorella Romano, Maria Isabel Caimi, Karina Cynthia Berná, Luisa Santangelo, María De La Paz |
| author_browse | Alvarado Pinedo, Maria Fiorella Berná, Luisa Caimi, Karina Cynthia Colombatti Olivieri, Maria Alejandra Cuerda, Maria Ximena Fresia, Pablo Graña, Martín Nagel, Ariel Gaston Romano, Maria Isabel Santangelo, María De La Paz |
| author_facet | Colombatti Olivieri, Maria Alejandra Fresia, Pablo Graña, Martín Cuerda, Maria Ximena Nagel, Ariel Gaston Alvarado Pinedo, Maria Fiorella Romano, Maria Isabel Caimi, Karina Cynthia Berná, Luisa Santangelo, María De La Paz |
| author_sort | Colombatti Olivieri, Maria Alejandra |
| collection | INTA Digital |
| description | In a previous study, we evaluated the degree of virulence of Mycobacterium avium subsp. paratuberculosis (Map) strains isolated from cattle in Argentina in a murine model. This assay allowed us to differentiate between high-virulent MapARG1347 and low-virulent MapARG1543 strains. To corroborate whether the differences in virulence could be attributed to genetic differences between the strains, we performed Whole Genome Sequencing and compared the genomes and gene content between them and determined the differences related to the reference strain MapK10. We found 233 SNPs/INDELS in one or both strains relative to Map K10. The two strains share most of the variations, but we found 15 mutations present in only one of the strains. Considering NS-SNP/INDELS that produced a severe effect in the coding sequence, we focus the analysis on four predicted proteins, putatively related to virulence. Survival of MapARG1347 strain in bMDM was higher than MapARG1543 and was more resistant to acidic pH and H2O2 stresses than MapK10. The genomic differences between the two strains found in genes MAP1203 (a putative peptidoglycan hydrolase), MAP0403 (a putative serine protease) MAP1003c (a member of the PE-PPE family) and MAP4152 (a putative mycofactocin binding protein) could contribute to explain the contrasting phenotype previously observed in mice models. |
| format | Artículo |
| id | INTA15156 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA151562023-09-08T15:38:31Z Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype Colombatti Olivieri, Maria Alejandra Fresia, Pablo Graña, Martín Cuerda, Maria Ximena Nagel, Ariel Gaston Alvarado Pinedo, Maria Fiorella Romano, Maria Isabel Caimi, Karina Cynthia Berná, Luisa Santangelo, María De La Paz Genomics Mycobacterium avium subsp. paratuberculosis Virulence Oxidative Stress Pathogenicity Phenotypes Genómica Virulencia Estrés Oxidativo Patogenicidad Fenotipos In a previous study, we evaluated the degree of virulence of Mycobacterium avium subsp. paratuberculosis (Map) strains isolated from cattle in Argentina in a murine model. This assay allowed us to differentiate between high-virulent MapARG1347 and low-virulent MapARG1543 strains. To corroborate whether the differences in virulence could be attributed to genetic differences between the strains, we performed Whole Genome Sequencing and compared the genomes and gene content between them and determined the differences related to the reference strain MapK10. We found 233 SNPs/INDELS in one or both strains relative to Map K10. The two strains share most of the variations, but we found 15 mutations present in only one of the strains. Considering NS-SNP/INDELS that produced a severe effect in the coding sequence, we focus the analysis on four predicted proteins, putatively related to virulence. Survival of MapARG1347 strain in bMDM was higher than MapARG1543 and was more resistant to acidic pH and H2O2 stresses than MapK10. The genomic differences between the two strains found in genes MAP1203 (a putative peptidoglycan hydrolase), MAP0403 (a putative serine protease) MAP1003c (a member of the PE-PPE family) and MAP4152 (a putative mycofactocin binding protein) could contribute to explain the contrasting phenotype previously observed in mice models. Instituto de Biotecnología Fil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fresia, Pablo. Institut Pasteur de Montevideo; Uruguay Fil: Graña, Martín. Institut Pasteur de Montevideo. Unidad de Bioinformática; Uruguay Fil: Cuerda, Maria Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Cuerda, Maria Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nagel, Ariel Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Nagel, Ariel Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Alvarado Pinedo, María Fiorella. Universidad de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Caimi, Karina Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Berná, Luisa. Institut Pasteur de Montevideo. Unidad de Biología Molecular; Uruguay Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2023-09-08T15:32:40Z 2023-09-08T15:32:40Z 2023-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/15156 https://www.sciencedirect.com/science/article/pii/S1472979222001366 1472-9792 https://doi.org/10.1016/j.tube.2022.102299 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001, Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco ?Una Salud? info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Tuberculosis 138 : 102299 (January 2023) |
| spellingShingle | Genomics Mycobacterium avium subsp. paratuberculosis Virulence Oxidative Stress Pathogenicity Phenotypes Genómica Virulencia Estrés Oxidativo Patogenicidad Fenotipos Colombatti Olivieri, Maria Alejandra Fresia, Pablo Graña, Martín Cuerda, Maria Ximena Nagel, Ariel Gaston Alvarado Pinedo, Maria Fiorella Romano, Maria Isabel Caimi, Karina Cynthia Berná, Luisa Santangelo, María De La Paz Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype |
| title | Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype |
| title_full | Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype |
| title_fullStr | Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype |
| title_full_unstemmed | Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype |
| title_short | Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype |
| title_sort | genomic comparison of two strains of mycobacterium avium subsp paratuberculosis with contrasting pathogenic phenotype |
| topic | Genomics Mycobacterium avium subsp. paratuberculosis Virulence Oxidative Stress Pathogenicity Phenotypes Genómica Virulencia Estrés Oxidativo Patogenicidad Fenotipos |
| url | http://hdl.handle.net/20.500.12123/15156 https://www.sciencedirect.com/science/article/pii/S1472979222001366 https://doi.org/10.1016/j.tube.2022.102299 |
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