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Genomic comparison of two strains of Mycobacterium avium subsp. paratuberculosis with contrasting pathogenic phenotype

In a previous study, we evaluated the degree of virulence of Mycobacterium avium subsp. paratuberculosis (Map) strains isolated from cattle in Argentina in a murine model. This assay allowed us to differentiate between high-virulent MapARG1347 and low-virulent MapARG1543 strains. To corroborate whet...

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Main Authors: Colombatti Olivieri, Maria Alejandra, Fresia, Pablo, Graña, Martín, Cuerda, Maria Ximena, Nagel, Ariel Gaston, Alvarado Pinedo, Maria Fiorella, Romano, Maria Isabel, Caimi, Karina Cynthia, Berná, Luisa, Santangelo, María De La Paz
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: Elsevier 2023
Subjects:
Genomics
Mycobacterium avium subsp. paratuberculosis
Virulence
Oxidative Stress
Pathogenicity
Phenotypes
Genómica
Virulencia
Estrés Oxidativo
Patogenicidad
Fenotipos
Online Access:http://hdl.handle.net/20.500.12123/15156
https://www.sciencedirect.com/science/article/pii/S1472979222001366
https://doi.org/10.1016/j.tube.2022.102299
Summary:In a previous study, we evaluated the degree of virulence of Mycobacterium avium subsp. paratuberculosis (Map) strains isolated from cattle in Argentina in a murine model. This assay allowed us to differentiate between high-virulent MapARG1347 and low-virulent MapARG1543 strains. To corroborate whether the differences in virulence could be attributed to genetic differences between the strains, we performed Whole Genome Sequencing and compared the genomes and gene content between them and determined the differences related to the reference strain MapK10. We found 233 SNPs/INDELS in one or both strains relative to Map K10. The two strains share most of the variations, but we found 15 mutations present in only one of the strains. Considering NS-SNP/INDELS that produced a severe effect in the coding sequence, we focus the analysis on four predicted proteins, putatively related to virulence. Survival of MapARG1347 strain in bMDM was higher than MapARG1543 and was more resistant to acidic pH and H2O2 stresses than MapK10. The genomic differences between the two strains found in genes MAP1203 (a putative peptidoglycan hydrolase), MAP0403 (a putative serine protease) MAP1003c (a member of the PE-PPE family) and MAP4152 (a putative mycofactocin binding protein) could contribute to explain the contrasting phenotype previously observed in mice models.

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