Transcriptional and metabolic changes associated to the infection by Fusarium verticillioides in maize inbreds with contrasting ear rot resistance

Fusarium verticillioides causes ear rot and grain mycotoxins in maize (Zea mays L.), which are harmful to human and animal health. Breeding and growing less susceptible plant genotypes is one alternative to reduce these detrimental effects. A better understanding of the resistance mechanisms would f...

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Main Authors: Campos Bermudez, Valeria Alina, Fauguel, Carolina Maria, Tronconi, Marcos A., Casati, Paula, Presello, Daniel Alberto, Andreo, Carlos Santiago
Format: Artículo
Language:Inglés
Published: 2017
Subjects:
Online Access:http://hdl.handle.net/20.500.12123/1451
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0061580
https://doi.org/10.1371/journal.pone.0061580
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author Campos Bermudez, Valeria Alina
Fauguel, Carolina Maria
Tronconi, Marcos A.
Casati, Paula
Presello, Daniel Alberto
Andreo, Carlos Santiago
author_browse Andreo, Carlos Santiago
Campos Bermudez, Valeria Alina
Casati, Paula
Fauguel, Carolina Maria
Presello, Daniel Alberto
Tronconi, Marcos A.
author_facet Campos Bermudez, Valeria Alina
Fauguel, Carolina Maria
Tronconi, Marcos A.
Casati, Paula
Presello, Daniel Alberto
Andreo, Carlos Santiago
author_sort Campos Bermudez, Valeria Alina
collection INTA Digital
description Fusarium verticillioides causes ear rot and grain mycotoxins in maize (Zea mays L.), which are harmful to human and animal health. Breeding and growing less susceptible plant genotypes is one alternative to reduce these detrimental effects. A better understanding of the resistance mechanisms would facilitate the implementation of strategic molecular agriculture to breeding of resistant germplasm. Our aim was to identify genes and metabolites that may be related to the Fusarium reaction in a resistant (L4637) and a susceptible (L4674) inbred. Gene expression data were obtained from microarray hybridizations in inoculated and non-inoculated kernels from both inbreds. Fungal inoculation did not produce considerable changes in gene expression and metabolites in L4637. Defense-related genes changed in L4674 kernels, responding specifically to the pathogen infection. These results indicate that L4637 resistance may be mainly due to constitutive defense mechanisms preventing fungal infection. These mechanisms seem to be poorly expressed in L4674; and despite the inoculation activate a defense response; this is not enough to prevent the disease progress in this susceptible line. Through this study, a global view of differential genes expressed and metabolites accumulated during resistance and susceptibility to F. verticillioides inoculation has been obtained, giving additional information about the mechanisms and pathways conferring resistance to this important disease in maize.
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spelling INTA14512019-06-06T17:45:54Z Transcriptional and metabolic changes associated to the infection by Fusarium verticillioides in maize inbreds with contrasting ear rot resistance Campos Bermudez, Valeria Alina Fauguel, Carolina Maria Tronconi, Marcos A. Casati, Paula Presello, Daniel Alberto Andreo, Carlos Santiago Maíz Maize Pathogenic Fungi Disease Resistance Fngal Diseases Mycotoxins Hongos Patógenos Resistencia a la Enfermedad Enfermedades Fungosas Micotoxinas Fusariosis Argentina Fusarium Verticillioides Fusarium verticillioides causes ear rot and grain mycotoxins in maize (Zea mays L.), which are harmful to human and animal health. Breeding and growing less susceptible plant genotypes is one alternative to reduce these detrimental effects. A better understanding of the resistance mechanisms would facilitate the implementation of strategic molecular agriculture to breeding of resistant germplasm. Our aim was to identify genes and metabolites that may be related to the Fusarium reaction in a resistant (L4637) and a susceptible (L4674) inbred. Gene expression data were obtained from microarray hybridizations in inoculated and non-inoculated kernels from both inbreds. Fungal inoculation did not produce considerable changes in gene expression and metabolites in L4637. Defense-related genes changed in L4674 kernels, responding specifically to the pathogen infection. These results indicate that L4637 resistance may be mainly due to constitutive defense mechanisms preventing fungal infection. These mechanisms seem to be poorly expressed in L4674; and despite the inoculation activate a defense response; this is not enough to prevent the disease progress in this susceptible line. Through this study, a global view of differential genes expressed and metabolites accumulated during resistance and susceptibility to F. verticillioides inoculation has been obtained, giving additional information about the mechanisms and pathways conferring resistance to this important disease in maize. Fil: Campos Bermudez, Valeria A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Fauguel, Carolina Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina Fil: Tronconi, Marcos A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Casati, Paula. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina Fil: Presello, Daniel Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina Fil: Andreo, Carlos Santiago. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina 2017-10-10T15:50:20Z 2017-10-10T15:50:20Z 2013 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1451 http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0061580 1932-6203 https://doi.org/10.1371/journal.pone.0061580 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf PLoS ONE 8(4): e61580. (2013)
spellingShingle Maíz
Maize
Pathogenic Fungi
Disease Resistance
Fngal Diseases
Mycotoxins
Hongos Patógenos
Resistencia a la Enfermedad
Enfermedades Fungosas
Micotoxinas
Fusariosis
Argentina
Fusarium Verticillioides
Campos Bermudez, Valeria Alina
Fauguel, Carolina Maria
Tronconi, Marcos A.
Casati, Paula
Presello, Daniel Alberto
Andreo, Carlos Santiago
Transcriptional and metabolic changes associated to the infection by Fusarium verticillioides in maize inbreds with contrasting ear rot resistance
title Transcriptional and metabolic changes associated to the infection by Fusarium verticillioides in maize inbreds with contrasting ear rot resistance
title_full Transcriptional and metabolic changes associated to the infection by Fusarium verticillioides in maize inbreds with contrasting ear rot resistance
title_fullStr Transcriptional and metabolic changes associated to the infection by Fusarium verticillioides in maize inbreds with contrasting ear rot resistance
title_full_unstemmed Transcriptional and metabolic changes associated to the infection by Fusarium verticillioides in maize inbreds with contrasting ear rot resistance
title_short Transcriptional and metabolic changes associated to the infection by Fusarium verticillioides in maize inbreds with contrasting ear rot resistance
title_sort transcriptional and metabolic changes associated to the infection by fusarium verticillioides in maize inbreds with contrasting ear rot resistance
topic Maíz
Maize
Pathogenic Fungi
Disease Resistance
Fngal Diseases
Mycotoxins
Hongos Patógenos
Resistencia a la Enfermedad
Enfermedades Fungosas
Micotoxinas
Fusariosis
Argentina
Fusarium Verticillioides
url http://hdl.handle.net/20.500.12123/1451
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0061580
https://doi.org/10.1371/journal.pone.0061580
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