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Assessment of the potential utility of different regions of Streptococcus uberis adhesion molecule (SUAM) for mastitis subunit vaccine development

Streptococcus uberis is one of the most prevalent pathogens causing clinical and subclinical mastitis worldwide. Among bacterial factors involved in intramammary infections caused by this organism, S. uberis adhesion molecule (SUAM) is one of the main virulence factors identified. This molecule is...

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Main Authors: Perrig, Melina Soledad, Veaute, Carolina Melania Isabel, Renna, María Sol, Pujato, Nazarena, Calvinho, Luis Fernando, Marcipar, Ivan Sergio, Barbagelata, Maria Sol
Format: info:ar-repo/semantics/artículo
Language:Inglés
Published: 2017
Subjects:
Enfermedades de los Animales
Bacteria
Streptococcus Uberis
Mastitis
Vacuna
Animal Diseases
Vaccines
Online Access:http://hdl.handle.net/20.500.12123/1440
http://www.sciencedirect.com/science/article/pii/S0882401016307173?via%3Dihub
https://doi.org/10.1016/j.micpath.2017.02.035
Summary:Streptococcus uberis is one of the most prevalent pathogens causing clinical and subclinical mastitis worldwide. Among bacterial factors involved in intramammary infections caused by this organism, S. uberis adhesion molecule (SUAM) is one of the main virulence factors identified. This molecule is involved in S. uberis internalization to mammary epithelial cells through lactoferrin (Lf) binding. The objective of this study was to evaluate SUAM properties as a potential subunit vaccine component for prevention of S. uberis mastitis. B epitope prediction analysis of SUAM sequence was used to identify potentially immunogenic regions. Since these regions were detected all along the gene, this criterion did not allow selecting a specific region as a potential immunogen. Hence, four fractions of SUAM (-1fr, 2fr, 3fr and 4fr), comprising most of the protein, were cloned and expressed. Every fraction elicited a humoral immune response in mice as predicted by bioinformatics analysis. SUAM-1fr generated antibodies with the highest recognition ability towards SUAM native protein. Moreover, antibodies against SUAM-1fr produced the highest proportion of internalization inhibition of S. uberis to mammary epithelial cells. In conclusion, SUAM immunogenic and functionally relevant regions were identified and allowed to propose SUAM-1fr as a potential candidate for a subunit vaccine for S. uberis mastitis prevention.

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