Assessment of the potential utility of different regions of Streptococcus uberis adhesion molecule (SUAM) for mastitis subunit vaccine development
Streptococcus uberis is one of the most prevalent pathogens causing clinical and subclinical mastitis worldwide. Among bacterial factors involved in intramammary infections caused by this organism, S. uberis adhesion molecule (SUAM) is one of the main virulence factors identified. This molecule is...
| Autores principales: | , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1440 http://www.sciencedirect.com/science/article/pii/S0882401016307173?via%3Dihub https://doi.org/10.1016/j.micpath.2017.02.035 |
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| author | Perrig, Melina Soledad Veaute, Carolina Melania Isabel Renna, María Sol Pujato, Nazarena Calvinho, Luis Fernando Marcipar, Ivan Sergio Barbagelata, Maria Sol |
| author_browse | Barbagelata, Maria Sol Calvinho, Luis Fernando Marcipar, Ivan Sergio Perrig, Melina Soledad Pujato, Nazarena Renna, María Sol Veaute, Carolina Melania Isabel |
| author_facet | Perrig, Melina Soledad Veaute, Carolina Melania Isabel Renna, María Sol Pujato, Nazarena Calvinho, Luis Fernando Marcipar, Ivan Sergio Barbagelata, Maria Sol |
| author_sort | Perrig, Melina Soledad |
| collection | INTA Digital |
| description | Streptococcus uberis is one of the most prevalent pathogens causing clinical and subclinical mastitis
worldwide. Among bacterial factors involved in intramammary infections caused by this organism,
S. uberis adhesion molecule (SUAM) is one of the main virulence factors identified. This molecule is
involved in S. uberis internalization to mammary epithelial cells through lactoferrin (Lf) binding. The
objective of this study was to evaluate SUAM properties as a potential subunit vaccine component for
prevention of S. uberis mastitis. B epitope prediction analysis of SUAM sequence was used to identify
potentially immunogenic regions. Since these regions were detected all along the gene, this criterion did
not allow selecting a specific region as a potential immunogen. Hence, four fractions of SUAM (-1fr, 2fr,
3fr and 4fr), comprising most of the protein, were cloned and expressed. Every fraction elicited a humoral
immune response in mice as predicted by bioinformatics analysis. SUAM-1fr generated antibodies with
the highest recognition ability towards SUAM native protein. Moreover, antibodies against SUAM-1fr
produced the highest proportion of internalization inhibition of S. uberis to mammary epithelial cells.
In conclusion, SUAM immunogenic and functionally relevant regions were identified and allowed to
propose SUAM-1fr as a potential candidate for a subunit vaccine for S. uberis mastitis prevention. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1440 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA14402019-03-22T15:00:10Z Assessment of the potential utility of different regions of Streptococcus uberis adhesion molecule (SUAM) for mastitis subunit vaccine development Perrig, Melina Soledad Veaute, Carolina Melania Isabel Renna, María Sol Pujato, Nazarena Calvinho, Luis Fernando Marcipar, Ivan Sergio Barbagelata, Maria Sol Enfermedades de los Animales Bacteria Streptococcus Uberis Mastitis Vacuna Animal Diseases Vaccines Streptococcus uberis is one of the most prevalent pathogens causing clinical and subclinical mastitis worldwide. Among bacterial factors involved in intramammary infections caused by this organism, S. uberis adhesion molecule (SUAM) is one of the main virulence factors identified. This molecule is involved in S. uberis internalization to mammary epithelial cells through lactoferrin (Lf) binding. The objective of this study was to evaluate SUAM properties as a potential subunit vaccine component for prevention of S. uberis mastitis. B epitope prediction analysis of SUAM sequence was used to identify potentially immunogenic regions. Since these regions were detected all along the gene, this criterion did not allow selecting a specific region as a potential immunogen. Hence, four fractions of SUAM (-1fr, 2fr, 3fr and 4fr), comprising most of the protein, were cloned and expressed. Every fraction elicited a humoral immune response in mice as predicted by bioinformatics analysis. SUAM-1fr generated antibodies with the highest recognition ability towards SUAM native protein. Moreover, antibodies against SUAM-1fr produced the highest proportion of internalization inhibition of S. uberis to mammary epithelial cells. In conclusion, SUAM immunogenic and functionally relevant regions were identified and allowed to propose SUAM-1fr as a potential candidate for a subunit vaccine for S. uberis mastitis prevention. EEA Rafaela Fil: Perrig, Melina Soledad. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Veaute, Carolina Melania Isabel. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina Fil: Renna, María Sol. Carolina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Pujato, Nazarena. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Marcipar, Ivan Sergio. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Barbagelata, Maria Sol. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas. Laboratorio de Tecnología Inmunológica; Argentina 2017-10-10T11:36:13Z 2017-10-10T11:36:13Z 2017-04 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1440 http://www.sciencedirect.com/science/article/pii/S0882401016307173?via%3Dihub 0882-4010 (Print) 1096-1208 (Online) https://doi.org/10.1016/j.micpath.2017.02.035 eng info:eu-repo/semantics/restrictedAccess application/pdf Microbial pathogenesis 105 : 273-279. (April 2017) |
| spellingShingle | Enfermedades de los Animales Bacteria Streptococcus Uberis Mastitis Vacuna Animal Diseases Vaccines Perrig, Melina Soledad Veaute, Carolina Melania Isabel Renna, María Sol Pujato, Nazarena Calvinho, Luis Fernando Marcipar, Ivan Sergio Barbagelata, Maria Sol Assessment of the potential utility of different regions of Streptococcus uberis adhesion molecule (SUAM) for mastitis subunit vaccine development |
| title | Assessment of the potential utility of different regions of Streptococcus uberis adhesion molecule (SUAM) for mastitis subunit vaccine development |
| title_full | Assessment of the potential utility of different regions of Streptococcus uberis adhesion molecule (SUAM) for mastitis subunit vaccine development |
| title_fullStr | Assessment of the potential utility of different regions of Streptococcus uberis adhesion molecule (SUAM) for mastitis subunit vaccine development |
| title_full_unstemmed | Assessment of the potential utility of different regions of Streptococcus uberis adhesion molecule (SUAM) for mastitis subunit vaccine development |
| title_short | Assessment of the potential utility of different regions of Streptococcus uberis adhesion molecule (SUAM) for mastitis subunit vaccine development |
| title_sort | assessment of the potential utility of different regions of streptococcus uberis adhesion molecule suam for mastitis subunit vaccine development |
| topic | Enfermedades de los Animales Bacteria Streptococcus Uberis Mastitis Vacuna Animal Diseases Vaccines |
| url | http://hdl.handle.net/20.500.12123/1440 http://www.sciencedirect.com/science/article/pii/S0882401016307173?via%3Dihub https://doi.org/10.1016/j.micpath.2017.02.035 |
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