BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle
Bovine leukemia virus (BLV) infections, causing persistent lymphocytosis and lethal lymphosarcoma in cattle, have reached high endemicity on dairy farms. We observed extensive inter-individual variation in the level of infection (LI) by assessing differences in proviral load in peripheral blood. Thi...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
2017
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/1430 http://onlinelibrary.wiley.com/doi/10.1111/age.12566/abstract;jsessionid=1A21B9D057520F287700321689693BE3.f04t02 https://doi.org/10.1111/age.12566 |
| _version_ | 1855034802025005056 |
|---|---|
| author | Carignano, Hugo Adrian Beribe, Maria Jose Caffaro, María Eugenia Amadio, Ariel Nani, Juan Pablo Gutierrez, Gerónimo Alvarez, Irene Trono, Karina Gabriela Miretti, Marcos Mateo Poli, Mario Andres |
| author_browse | Alvarez, Irene Amadio, Ariel Beribe, Maria Jose Caffaro, María Eugenia Carignano, Hugo Adrian Gutierrez, Gerónimo Miretti, Marcos Mateo Nani, Juan Pablo Poli, Mario Andres Trono, Karina Gabriela |
| author_facet | Carignano, Hugo Adrian Beribe, Maria Jose Caffaro, María Eugenia Amadio, Ariel Nani, Juan Pablo Gutierrez, Gerónimo Alvarez, Irene Trono, Karina Gabriela Miretti, Marcos Mateo Poli, Mario Andres |
| author_sort | Carignano, Hugo Adrian |
| collection | INTA Digital |
| description | Bovine leukemia virus (BLV) infections, causing persistent lymphocytosis and lethal lymphosarcoma in cattle, have reached high endemicity on dairy farms. We observed extensive inter-individual variation in the level of infection (LI) by assessing differences in proviral load in peripheral blood. This phenotypic variation appears to be determined by host genetics variants, especially those located in the BoLA-DRB3 MHCII molecule. We performed an association study using sequencing-based typed BOLA-DRB3 alleles from over 800 Holstein and Holstein × Jersey cows considering LI in vivo and accounting for filial relationships. The DBR3*0902 allele was associated with a low level of infection (LLI) (<1% of circulating infected B-cells), whereas the DRB3*1001 and DRB3*1201 alleles were related to a high level of infection (HLI). We found evidence that 13 polymorphic positions located in the pockets of the peptide-binding cleft of the BOLA-DRB3 alleles were associated with LI. DRB3*0902 had unique haplotypes for each of the pockets: Ser13-Glu70-Arg71-Glu74 (pocket 4), Ser11-Ser30 (pocket 6), Glu28-Trp61-Arg71 (pocket 7) and Asn37-Asp57 (pocket 9), and all of them were significantly associated with LLI. Conversely, Lys13-Arg70-Ala71-Ala74 and Ser13-Arg70-Ala71-Ala74, corresponding to the DRB3*1001 and *1201 alleles respectively, were associated with HLI. We showed that the specific amino acid pattern in the DRB3*0902 peptide-binding cleft may be related to the set point of a very low proviral load level in adult cows. Moreover, we identified two BOLA-DRB3 alleles associated with a HLI, which is compatible with a highly contagious profile. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1430 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA14302020-04-02T15:38:09Z BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle Carignano, Hugo Adrian Beribe, Maria Jose Caffaro, María Eugenia Amadio, Ariel Nani, Juan Pablo Gutierrez, Gerónimo Alvarez, Irene Trono, Karina Gabriela Miretti, Marcos Mateo Poli, Mario Andres Enfermedades de los Animales Ganado Bovino Animal Diseases Cattle Bovine Leukaemia Virus Breeds (Animals) Crossbreeding Polymorphism Genetics Virus Leucemia Bovina Razas (Animales) Cruzamiento Polimorfismo Genética Raza Holstein Raza Jersey Bovine leukemia virus (BLV) infections, causing persistent lymphocytosis and lethal lymphosarcoma in cattle, have reached high endemicity on dairy farms. We observed extensive inter-individual variation in the level of infection (LI) by assessing differences in proviral load in peripheral blood. This phenotypic variation appears to be determined by host genetics variants, especially those located in the BoLA-DRB3 MHCII molecule. We performed an association study using sequencing-based typed BOLA-DRB3 alleles from over 800 Holstein and Holstein × Jersey cows considering LI in vivo and accounting for filial relationships. The DBR3*0902 allele was associated with a low level of infection (LLI) (<1% of circulating infected B-cells), whereas the DRB3*1001 and DRB3*1201 alleles were related to a high level of infection (HLI). We found evidence that 13 polymorphic positions located in the pockets of the peptide-binding cleft of the BOLA-DRB3 alleles were associated with LI. DRB3*0902 had unique haplotypes for each of the pockets: Ser13-Glu70-Arg71-Glu74 (pocket 4), Ser11-Ser30 (pocket 6), Glu28-Trp61-Arg71 (pocket 7) and Asn37-Asp57 (pocket 9), and all of them were significantly associated with LLI. Conversely, Lys13-Arg70-Ala71-Ala74 and Ser13-Arg70-Ala71-Ala74, corresponding to the DRB3*1001 and *1201 alleles respectively, were associated with HLI. We showed that the specific amino acid pattern in the DRB3*0902 peptide-binding cleft may be related to the set point of a very low proviral load level in adult cows. Moreover, we identified two BOLA-DRB3 alleles associated with a HLI, which is compatible with a highly contagious profile. Fil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Beribe, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Pergamino; Argentina Fil: Caffaro, María Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nani, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Alvarez, Irene. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Miretti, Marcos Mateo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas | Universidad Nacional de Misiones. Instituto de Biología Subtropical. Instituto de Biología Subtropical - Nodo Posadas; Argentina Fil: Poli, Mario AndresInstituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética "Ewald A. Favret"; Argentina 2017-10-06T14:35:02Z 2017-10-06T14:35:02Z 2017-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1430 http://onlinelibrary.wiley.com/doi/10.1111/age.12566/abstract;jsessionid=1A21B9D057520F287700321689693BE3.f04t02 0268-9146 (Print) 1365-2052 (Online) https://doi.org/10.1111/age.12566 eng info:eu-repo/semantics/restrictedAccess application/pdf Animal genetics 48 (4) : 420–430. (August 2017) |
| spellingShingle | Enfermedades de los Animales Ganado Bovino Animal Diseases Cattle Bovine Leukaemia Virus Breeds (Animals) Crossbreeding Polymorphism Genetics Virus Leucemia Bovina Razas (Animales) Cruzamiento Polimorfismo Genética Raza Holstein Raza Jersey Carignano, Hugo Adrian Beribe, Maria Jose Caffaro, María Eugenia Amadio, Ariel Nani, Juan Pablo Gutierrez, Gerónimo Alvarez, Irene Trono, Karina Gabriela Miretti, Marcos Mateo Poli, Mario Andres BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle |
| title | BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle |
| title_full | BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle |
| title_fullStr | BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle |
| title_full_unstemmed | BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle |
| title_short | BOLA-DRB3 gene polymorphisms influence bovine leukaemia virus infection levels in Holstein and Holstein × Jersey crossbreed dairy cattle |
| title_sort | bola drb3 gene polymorphisms influence bovine leukaemia virus infection levels in holstein and holstein jersey crossbreed dairy cattle |
| topic | Enfermedades de los Animales Ganado Bovino Animal Diseases Cattle Bovine Leukaemia Virus Breeds (Animals) Crossbreeding Polymorphism Genetics Virus Leucemia Bovina Razas (Animales) Cruzamiento Polimorfismo Genética Raza Holstein Raza Jersey |
| url | http://hdl.handle.net/20.500.12123/1430 http://onlinelibrary.wiley.com/doi/10.1111/age.12566/abstract;jsessionid=1A21B9D057520F287700321689693BE3.f04t02 https://doi.org/10.1111/age.12566 |
| work_keys_str_mv | AT carignanohugoadrian boladrb3genepolymorphismsinfluencebovineleukaemiavirusinfectionlevelsinholsteinandholsteinjerseycrossbreeddairycattle AT beribemariajose boladrb3genepolymorphismsinfluencebovineleukaemiavirusinfectionlevelsinholsteinandholsteinjerseycrossbreeddairycattle AT caffaromariaeugenia boladrb3genepolymorphismsinfluencebovineleukaemiavirusinfectionlevelsinholsteinandholsteinjerseycrossbreeddairycattle AT amadioariel boladrb3genepolymorphismsinfluencebovineleukaemiavirusinfectionlevelsinholsteinandholsteinjerseycrossbreeddairycattle AT nanijuanpablo boladrb3genepolymorphismsinfluencebovineleukaemiavirusinfectionlevelsinholsteinandholsteinjerseycrossbreeddairycattle AT gutierrezgeronimo boladrb3genepolymorphismsinfluencebovineleukaemiavirusinfectionlevelsinholsteinandholsteinjerseycrossbreeddairycattle AT alvarezirene boladrb3genepolymorphismsinfluencebovineleukaemiavirusinfectionlevelsinholsteinandholsteinjerseycrossbreeddairycattle AT tronokarinagabriela boladrb3genepolymorphismsinfluencebovineleukaemiavirusinfectionlevelsinholsteinandholsteinjerseycrossbreeddairycattle AT mirettimarcosmateo boladrb3genepolymorphismsinfluencebovineleukaemiavirusinfectionlevelsinholsteinandholsteinjerseycrossbreeddairycattle AT polimarioandres boladrb3genepolymorphismsinfluencebovineleukaemiavirusinfectionlevelsinholsteinandholsteinjerseycrossbreeddairycattle |