A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction
Although mammals are still the main source of polyclonal antibodies production, the use of egg yolk immunoglobulins (IgY) is increasing, and thus improvement of its safety to reduce undesired reactions is needed. Removal of IgY constant domains by pepsin enzymatic treatment is expected to reduce pot...
| Main Authors: | , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Elsevier
2023
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/13964 https://www.sciencedirect.com/science/article/pii/S1570023222003907 https://doi.org/10.1016/j.jchromb.2022.123486 |
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| author | González Viacava, Myriam Belén Leiva, Carlos Leónidas Tavarone, Maria Eugenia Dokmetjian, José Christian Cascone, Osvaldo Chacana, Pablo Fingermann, Matías |
| author_browse | Cascone, Osvaldo Chacana, Pablo Dokmetjian, José Christian Fingermann, Matías González Viacava, Myriam Belén Leiva, Carlos Leónidas Tavarone, Maria Eugenia |
| author_facet | González Viacava, Myriam Belén Leiva, Carlos Leónidas Tavarone, Maria Eugenia Dokmetjian, José Christian Cascone, Osvaldo Chacana, Pablo Fingermann, Matías |
| author_sort | González Viacava, Myriam Belén |
| collection | INTA Digital |
| description | Although mammals are still the main source of polyclonal antibodies production, the use of egg yolk immunoglobulins (IgY) is increasing, and thus improvement of its safety to reduce undesired reactions is needed. Removal of IgY constant domains by pepsin enzymatic treatment is expected to reduce potential adverse effects derived from the parenteral administration of these antibodies, while also increasing its distribution volume. Current Fab’ production requires the previous purification of IgY to be used as starting material. In this context, relevant economic benefits may be gained if initial IgY purification could be avoided, by using rawer starting materials. In this work, a three-step process for Fab’ production from crude egg yolk water-soluble fractions is described using scalable and simple low-cost technologies such as ultrafiltration and anion exchange chromatography. The overall process yield of 33% highly pure Fab’ from water-soluble fractions favorably compares to the manufacture of related medicinal products from mammalian antibodies, such as antivenoms. |
| format | info:ar-repo/semantics/artículo |
| id | INTA13964 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA139642023-02-14T11:58:02Z A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction González Viacava, Myriam Belén Leiva, Carlos Leónidas Tavarone, Maria Eugenia Dokmetjian, José Christian Cascone, Osvaldo Chacana, Pablo Fingermann, Matías Egg Yolk Antibodies Purification Chromatography Yema de Huevo Anticuerpos Purificación Cromatografía Although mammals are still the main source of polyclonal antibodies production, the use of egg yolk immunoglobulins (IgY) is increasing, and thus improvement of its safety to reduce undesired reactions is needed. Removal of IgY constant domains by pepsin enzymatic treatment is expected to reduce potential adverse effects derived from the parenteral administration of these antibodies, while also increasing its distribution volume. Current Fab’ production requires the previous purification of IgY to be used as starting material. In this context, relevant economic benefits may be gained if initial IgY purification could be avoided, by using rawer starting materials. In this work, a three-step process for Fab’ production from crude egg yolk water-soluble fractions is described using scalable and simple low-cost technologies such as ultrafiltration and anion exchange chromatography. The overall process yield of 33% highly pure Fab’ from water-soluble fractions favorably compares to the manufacture of related medicinal products from mammalian antibodies, such as antivenoms. Instituto de Patobiología Fil: González Viacava, Myriam Belén. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; Argentina Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tavarone, Maria Eugenia. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; Argentina Fil: Dokmetjian, José Christian. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; Argentina Fil: Cascone, Osvaldo. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; Argentina Fil: Cascone, Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina Fil: Cascone, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fingermann, Matías. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; Argentina 2023-02-14T11:52:24Z 2023-02-14T11:52:24Z 2022-11 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/13964 https://www.sciencedirect.com/science/article/pii/S1570023222003907 1873-376X https://doi.org/10.1016/j.jchromb.2022.123486 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Journal of Chromatography B 1211 : 123486 (Noviembre 2022) |
| spellingShingle | Egg Yolk Antibodies Purification Chromatography Yema de Huevo Anticuerpos Purificación Cromatografía González Viacava, Myriam Belén Leiva, Carlos Leónidas Tavarone, Maria Eugenia Dokmetjian, José Christian Cascone, Osvaldo Chacana, Pablo Fingermann, Matías A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction |
| title | A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction |
| title_full | A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction |
| title_fullStr | A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction |
| title_full_unstemmed | A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction |
| title_short | A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction |
| title_sort | simple and economic three step process for producing highly purified fab fragments directly from the egg yolk water soluble fraction |
| topic | Egg Yolk Antibodies Purification Chromatography Yema de Huevo Anticuerpos Purificación Cromatografía |
| url | http://hdl.handle.net/20.500.12123/13964 https://www.sciencedirect.com/science/article/pii/S1570023222003907 https://doi.org/10.1016/j.jchromb.2022.123486 |
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