Pre-harvest strategy for reducing aflatoxin accumulation during storage of maize in Argentina
Maize (Zea mays L.) is an important crop in Argentina. Aspergillus flavus may infect this crop at growing stage and the harvested kernels can be contaminated with aflatoxins (AFs), whose levels may increase during storage. In Argentina, silo bags, a hermetic type of storage system, are widely used....
| Main Authors: | , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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Elsevier
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/12776 https://www.sciencedirect.com/science/article/pii/S0168160522003592 https://doi.org/10.1016/j.ijfoodmicro.2022.109887 |
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| author | Alaniz Zanon, María Silvina Bossa, Marianela Chiotta, María Laura Oddino, Claudio Giovanini, Diego Cardoso, Marcelo Leandro Bartosik, Ricardo Enrique Chulze, Sofía Noemí |
| author_browse | Alaniz Zanon, María Silvina Bartosik, Ricardo Enrique Bossa, Marianela Cardoso, Marcelo Leandro Chiotta, María Laura Chulze, Sofía Noemí Giovanini, Diego Oddino, Claudio |
| author_facet | Alaniz Zanon, María Silvina Bossa, Marianela Chiotta, María Laura Oddino, Claudio Giovanini, Diego Cardoso, Marcelo Leandro Bartosik, Ricardo Enrique Chulze, Sofía Noemí |
| author_sort | Alaniz Zanon, María Silvina |
| collection | INTA Digital |
| description | Maize (Zea mays L.) is an important crop in Argentina. Aspergillus flavus may infect this crop at growing stage and the harvested kernels can be contaminated with aflatoxins (AFs), whose levels may increase during storage. In Argentina, silo bags, a hermetic type of storage system, are widely used. Biocontrol based on competitive exclusion by atoxigenic A. flavus strains is a useful tool for AFs management at pre-harvest stage. The aim of the present study was to evaluate the effect of pre-harvest biocontrol treatments on aflatoxin B1 (AFB1) accumulation in maize stored in silo bags during 3 and 6 months. Three bioformulations based on A. flavus AFCHG2 and ARG5/30 strains were applied during field trials as single and mixed inocula. Harvested kernels were stored in non-hermetic and hermetic silo bags. At initial time (t0), 3 and 6 months (t3 and t6) the following parameters were evaluated: percentage of damaged kernels, moisture content, water activity, Aspergillus section Flavi incidence, relative humidity, O2 and CO2 levels into the silo bags, and AFB1 levels. The biocontrol strains included in the 3 bioformulations were able to infect maize kernels during the field trial and displaced native toxigenic isolates. At t0 control plots showed 10.9 ± 0.4 μg/kg of AFB1 while no AFs were detected in all the treatments. Along the storage assay AFB1 levels varied from not detected (<1 μg/kg) to 20.1 ± 0.8 μg/kg. Hermetic bags were better than non-hermetic bags in preventing AFB1 accumulation. Both single and mixed inocula were effective to control AFB1 accumulation in maize kernels during 3 and 6 months. AFB1 was not detected in kernels from the treatment at field stage with AFCHG2 + ARG5/30 after 6 months of storage into hermetic bags. The application of the biocontrol agents at field stage is an appropriate tool to reduce AFB1 accumulation under storage in hermetic silo bags. This is the first report on biocontrol strategy based on native atoxigenic strains applied at pre-harvest stage to reduce AFB1 accumulation during storage in Argentina. |
| format | Artículo |
| id | INTA12776 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA127762022-09-05T17:33:09Z Pre-harvest strategy for reducing aflatoxin accumulation during storage of maize in Argentina Alaniz Zanon, María Silvina Bossa, Marianela Chiotta, María Laura Oddino, Claudio Giovanini, Diego Cardoso, Marcelo Leandro Bartosik, Ricardo Enrique Chulze, Sofía Noemí Maíz Aspergillus Flavus Aflatoxinas Almacenamiento Atmósfera Controlada Control Biológico Argentina Maize Aflatoxins Controlled Atmosphere Storage Biological Control Maize (Zea mays L.) is an important crop in Argentina. Aspergillus flavus may infect this crop at growing stage and the harvested kernels can be contaminated with aflatoxins (AFs), whose levels may increase during storage. In Argentina, silo bags, a hermetic type of storage system, are widely used. Biocontrol based on competitive exclusion by atoxigenic A. flavus strains is a useful tool for AFs management at pre-harvest stage. The aim of the present study was to evaluate the effect of pre-harvest biocontrol treatments on aflatoxin B1 (AFB1) accumulation in maize stored in silo bags during 3 and 6 months. Three bioformulations based on A. flavus AFCHG2 and ARG5/30 strains were applied during field trials as single and mixed inocula. Harvested kernels were stored in non-hermetic and hermetic silo bags. At initial time (t0), 3 and 6 months (t3 and t6) the following parameters were evaluated: percentage of damaged kernels, moisture content, water activity, Aspergillus section Flavi incidence, relative humidity, O2 and CO2 levels into the silo bags, and AFB1 levels. The biocontrol strains included in the 3 bioformulations were able to infect maize kernels during the field trial and displaced native toxigenic isolates. At t0 control plots showed 10.9 ± 0.4 μg/kg of AFB1 while no AFs were detected in all the treatments. Along the storage assay AFB1 levels varied from not detected (<1 μg/kg) to 20.1 ± 0.8 μg/kg. Hermetic bags were better than non-hermetic bags in preventing AFB1 accumulation. Both single and mixed inocula were effective to control AFB1 accumulation in maize kernels during 3 and 6 months. AFB1 was not detected in kernels from the treatment at field stage with AFCHG2 + ARG5/30 after 6 months of storage into hermetic bags. The application of the biocontrol agents at field stage is an appropriate tool to reduce AFB1 accumulation under storage in hermetic silo bags. This is the first report on biocontrol strategy based on native atoxigenic strains applied at pre-harvest stage to reduce AFB1 accumulation during storage in Argentina. EEA Balcarce Fil: Alaniz Zanon, María Silvina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina. Universidad Nacional de Río Cuarto; Argentina. Instituto de Investigación en Micología y Micotoxicología; Argentina. Fil: Bossa, Marianela. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina. Universidad Nacional de Río Cuarto; Argentina. Instituto de Investigación en Micología y Micotoxicología; Argentina. Fil: Chiotta, María Laura. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina. Universidad Nacional de Río Cuarto; Argentina. Instituto de Investigación en Micología y Micotoxicología; Argentina. Fil: Oddino, Claudio. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina. Universidad Nacional de Río Cuarto; Argentina. Instituto de Investigación en Micología y Micotoxicología; Argentina. Fil: Giovanini, Diego. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina. Universidad Nacional de Río Cuarto; Argentina. Instituto de Investigación en Micología y Micotoxicología; Argentina. Fil: Cardoso, Marcelo Leandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Bartosik, Ricardo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina. Fil: Chulze, Sofía Noemí. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina. Universidad Nacional de Río Cuarto; Argentina. Instituto de Investigación en Micología y Micotoxicología; Argentina. 2022-09-05T17:26:30Z 2022-09-05T17:26:30Z 2022-08-27 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12776 https://www.sciencedirect.com/science/article/pii/S0168160522003592 0168-1605 https://doi.org/10.1016/j.ijfoodmicro.2022.109887 eng info:eu-repo/semantics/restrictedAccess application/pdf Elsevier International Journal of Food Microbiology 380 : 109887 (November 2022) |
| spellingShingle | Maíz Aspergillus Flavus Aflatoxinas Almacenamiento Atmósfera Controlada Control Biológico Argentina Maize Aflatoxins Controlled Atmosphere Storage Biological Control Alaniz Zanon, María Silvina Bossa, Marianela Chiotta, María Laura Oddino, Claudio Giovanini, Diego Cardoso, Marcelo Leandro Bartosik, Ricardo Enrique Chulze, Sofía Noemí Pre-harvest strategy for reducing aflatoxin accumulation during storage of maize in Argentina |
| title | Pre-harvest strategy for reducing aflatoxin accumulation during storage of maize in Argentina |
| title_full | Pre-harvest strategy for reducing aflatoxin accumulation during storage of maize in Argentina |
| title_fullStr | Pre-harvest strategy for reducing aflatoxin accumulation during storage of maize in Argentina |
| title_full_unstemmed | Pre-harvest strategy for reducing aflatoxin accumulation during storage of maize in Argentina |
| title_short | Pre-harvest strategy for reducing aflatoxin accumulation during storage of maize in Argentina |
| title_sort | pre harvest strategy for reducing aflatoxin accumulation during storage of maize in argentina |
| topic | Maíz Aspergillus Flavus Aflatoxinas Almacenamiento Atmósfera Controlada Control Biológico Argentina Maize Aflatoxins Controlled Atmosphere Storage Biological Control |
| url | http://hdl.handle.net/20.500.12123/12776 https://www.sciencedirect.com/science/article/pii/S0168160522003592 https://doi.org/10.1016/j.ijfoodmicro.2022.109887 |
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