Characterization of a new Helicoverpa armigera nucleopolyhedrovirus variant causing epizootic on a previously unreported host, Helicoverpa gelotopoeon (Lepidoptera: Noctuidae)
This paper reports the first biological and molecular characterization of a nucleopolyhedrovirus isolated from the soybean and cotton pest Helicoverpa gelotopoeon. Studies were performed following a virus outbreak in a rearing facility and in wild H. gelotopoeon populations in Córdoba, Argentina....
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1258 http://www.sciencedirect.com/science/article/pii/S0022201115300082 https://doi.org/10.1016/j.jip.2015.08.009 |
| Sumario: | This paper reports the first biological and molecular characterization of a nucleopolyhedrovirus isolated
from the soybean and cotton pest Helicoverpa gelotopoeon. Studies were performed following a virus outbreak
in a rearing facility and in wild H. gelotopoeon populations in Córdoba, Argentina. Host identity was
corroborated by partial sequencing of the COI gene. Scanning electron microscope observations of purified
OBs revealed their polyhedral morphology and an average diameter of 0.89 ± 0.14 lm. Ultrathin
sections of infected larvae examined by transmission electron microscopy showed the intranuclear
occurrence of polyhedra and virus particles in fat body cells. Nucleocapsids were singly enveloped.
Phylogenetic analysis of lef-8, lef-9, polh, orf5/5b and hr3-orf62 viral sequences identified this new NPV
isolate (hereafter HegeSNPV) as a variant of Helicoverpa armigera nucleopolyhedrovirus (HearNPV).
Furthermore, HegeSNPV was closely related to the so-called ‘‘HzSNPV Group” within HearNPV, although
having particular characteristics. |
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