Characterization of a new Helicoverpa armigera nucleopolyhedrovirus variant causing epizootic on a previously unreported host, Helicoverpa gelotopoeon (Lepidoptera: Noctuidae)
This paper reports the first biological and molecular characterization of a nucleopolyhedrovirus isolated from the soybean and cotton pest Helicoverpa gelotopoeon. Studies were performed following a virus outbreak in a rearing facility and in wild H. gelotopoeon populations in Córdoba, Argentina....
| Autores principales: | , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/1258 http://www.sciencedirect.com/science/article/pii/S0022201115300082 https://doi.org/10.1016/j.jip.2015.08.009 |
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| author | Ferrelli, Maria Leticia Taibo, Catalina Beatriz Fichetti, Patricia Sciocco, Alicia Ines Arneodo Larochette, Joel Demian |
| author_browse | Arneodo Larochette, Joel Demian Ferrelli, Maria Leticia Fichetti, Patricia Sciocco, Alicia Ines Taibo, Catalina Beatriz |
| author_facet | Ferrelli, Maria Leticia Taibo, Catalina Beatriz Fichetti, Patricia Sciocco, Alicia Ines Arneodo Larochette, Joel Demian |
| author_sort | Ferrelli, Maria Leticia |
| collection | INTA Digital |
| description | This paper reports the first biological and molecular characterization of a nucleopolyhedrovirus isolated
from the soybean and cotton pest Helicoverpa gelotopoeon. Studies were performed following a virus outbreak
in a rearing facility and in wild H. gelotopoeon populations in Córdoba, Argentina. Host identity was
corroborated by partial sequencing of the COI gene. Scanning electron microscope observations of purified
OBs revealed their polyhedral morphology and an average diameter of 0.89 ± 0.14 lm. Ultrathin
sections of infected larvae examined by transmission electron microscopy showed the intranuclear
occurrence of polyhedra and virus particles in fat body cells. Nucleocapsids were singly enveloped.
Phylogenetic analysis of lef-8, lef-9, polh, orf5/5b and hr3-orf62 viral sequences identified this new NPV
isolate (hereafter HegeSNPV) as a variant of Helicoverpa armigera nucleopolyhedrovirus (HearNPV).
Furthermore, HegeSNPV was closely related to the so-called ‘‘HzSNPV Group” within HearNPV, although
having particular characteristics. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1258 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA12582019-03-22T14:06:16Z Characterization of a new Helicoverpa armigera nucleopolyhedrovirus variant causing epizootic on a previously unreported host, Helicoverpa gelotopoeon (Lepidoptera: Noctuidae) Ferrelli, Maria Leticia Taibo, Catalina Beatriz Fichetti, Patricia Sciocco, Alicia Ines Arneodo Larochette, Joel Demian Plagas de Plantas Helicoverpa Armigera Lepidóptera Pests of Plants Helicoverpa Gelotopoeon This paper reports the first biological and molecular characterization of a nucleopolyhedrovirus isolated from the soybean and cotton pest Helicoverpa gelotopoeon. Studies were performed following a virus outbreak in a rearing facility and in wild H. gelotopoeon populations in Córdoba, Argentina. Host identity was corroborated by partial sequencing of the COI gene. Scanning electron microscope observations of purified OBs revealed their polyhedral morphology and an average diameter of 0.89 ± 0.14 lm. Ultrathin sections of infected larvae examined by transmission electron microscopy showed the intranuclear occurrence of polyhedra and virus particles in fat body cells. Nucleocapsids were singly enveloped. Phylogenetic analysis of lef-8, lef-9, polh, orf5/5b and hr3-orf62 viral sequences identified this new NPV isolate (hereafter HegeSNPV) as a variant of Helicoverpa armigera nucleopolyhedrovirus (HearNPV). Furthermore, HegeSNPV was closely related to the so-called ‘‘HzSNPV Group” within HearNPV, although having particular characteristics. Inst. de Microbiología y Zoología Agrícola IMyZA Fil: Ferrelli, Maria Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina Fil: Taibo, Catalina Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Centro de Investigación en Ciencias Veterinarias y Agronómicas. Servicio de Microscopía; Argentina Fil: Fichetti, Patricia. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Departamento de Protección Vegetal; Argentina Fil: Sciocco, Alicia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Microbiología y Zoología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-09-19T17:59:25Z 2017-09-19T17:59:25Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1258 http://www.sciencedirect.com/science/article/pii/S0022201115300082 0022-2011 https://doi.org/10.1016/j.jip.2015.08.009 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of invertebrate pathology 138 : 89-93. (July 2016) |
| spellingShingle | Plagas de Plantas Helicoverpa Armigera Lepidóptera Pests of Plants Helicoverpa Gelotopoeon Ferrelli, Maria Leticia Taibo, Catalina Beatriz Fichetti, Patricia Sciocco, Alicia Ines Arneodo Larochette, Joel Demian Characterization of a new Helicoverpa armigera nucleopolyhedrovirus variant causing epizootic on a previously unreported host, Helicoverpa gelotopoeon (Lepidoptera: Noctuidae) |
| title | Characterization of a new Helicoverpa armigera nucleopolyhedrovirus variant causing epizootic on a previously unreported host, Helicoverpa gelotopoeon (Lepidoptera: Noctuidae) |
| title_full | Characterization of a new Helicoverpa armigera nucleopolyhedrovirus variant causing epizootic on a previously unreported host, Helicoverpa gelotopoeon (Lepidoptera: Noctuidae) |
| title_fullStr | Characterization of a new Helicoverpa armigera nucleopolyhedrovirus variant causing epizootic on a previously unreported host, Helicoverpa gelotopoeon (Lepidoptera: Noctuidae) |
| title_full_unstemmed | Characterization of a new Helicoverpa armigera nucleopolyhedrovirus variant causing epizootic on a previously unreported host, Helicoverpa gelotopoeon (Lepidoptera: Noctuidae) |
| title_short | Characterization of a new Helicoverpa armigera nucleopolyhedrovirus variant causing epizootic on a previously unreported host, Helicoverpa gelotopoeon (Lepidoptera: Noctuidae) |
| title_sort | characterization of a new helicoverpa armigera nucleopolyhedrovirus variant causing epizootic on a previously unreported host helicoverpa gelotopoeon lepidoptera noctuidae |
| topic | Plagas de Plantas Helicoverpa Armigera Lepidóptera Pests of Plants Helicoverpa Gelotopoeon |
| url | http://hdl.handle.net/20.500.12123/1258 http://www.sciencedirect.com/science/article/pii/S0022201115300082 https://doi.org/10.1016/j.jip.2015.08.009 |
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