Comparison of two coprological methods for the diagnosis of Eurytrema ssp. in cattle and sheep
Eurytrematosis is a disease caused by flukes of the genus Eurytrema. These parasites infect the pancreatic ducts of a wide variety of species, including cattle, sheep and humans. Diagnosing eurytrematosis through the analysis of faecal samples can be difficult because most of the available techni...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
Cambridge University Press
2022
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/12436 https://www.cambridge.org/core/journals/journal-of-helminthology/article/abs/comparison-of-two-coprological-methods-for-the-diagnosis-of-eurytrema-ssp-in-cattle-and-sheep/F240BADD6A4FC46156DFA71B9ADACBBC https://doi.org/10.1017/S0022149X22000414 |
| _version_ | 1855484972967657472 |
|---|---|
| author | Olmos, Leandro Hipolito Pantiu, Andrea Julia Avellaneda Cáceres, Agustín Valencia, P.N. Cayo, P.N. Signorini Porchiett, Marcelo Lisandro Micheloud, Juan Francisco |
| author_browse | Avellaneda Cáceres, Agustín Cayo, P.N. Micheloud, Juan Francisco Olmos, Leandro Hipolito Pantiu, Andrea Julia Signorini Porchiett, Marcelo Lisandro Valencia, P.N. |
| author_facet | Olmos, Leandro Hipolito Pantiu, Andrea Julia Avellaneda Cáceres, Agustín Valencia, P.N. Cayo, P.N. Signorini Porchiett, Marcelo Lisandro Micheloud, Juan Francisco |
| author_sort | Olmos, Leandro Hipolito |
| collection | INTA Digital |
| description | Eurytrematosis is a disease caused by flukes of the genus Eurytrema. These parasites infect the
pancreatic ducts of a wide variety of species, including cattle, sheep and humans. Diagnosing
eurytrematosis through the analysis of faecal samples can be difficult because most of the available
techniques are considered of low sensitivity. In this context, a modification of the Dennis,
Stone and Swanson technique (Belem Sedimentation Technique, BST) was previously developed
to increase the probability of detecting infected animals; nevertheless, the values of eggs per
gram obtained using the modified technique are generally low. We proposed a modification
of the this technique (MBST), to increase the sensitivity and detection rate of infected animals.
The objective of this work was to describe MBST and compare it with BST. Faecal samples of
212 clinically healthy animals (174 from cattle and 38 from sheep) from 20 farms were taken by
the intra-rectal route and stored at 4°C. The samples were processed using BST and MBST.
Positive samples amounted to 55 (25.9%) using BST and 121 (57.1%) using MBST. In the simples
from cattle, 52 (29.8%) and 107 (61.4%) were positive in BST and MBST, respectively. In
sheep, three (7.8%) and 14 (36.8%) positive samples were obtained in BST and MBST,
respectively.The results obtained using the two methods were significantly different, indicating
a lack of agreement between their findings. The results suggest that MBST is a more sensitive
method to detect Eurytrema spp. eggs in faeces than BST. |
| format | Artículo |
| id | INTA12436 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Cambridge University Press |
| publisherStr | Cambridge University Press |
| record_format | dspace |
| spelling | INTA124362022-07-29T10:50:07Z Comparison of two coprological methods for the diagnosis of Eurytrema ssp. in cattle and sheep Olmos, Leandro Hipolito Pantiu, Andrea Julia Avellaneda Cáceres, Agustín Valencia, P.N. Cayo, P.N. Signorini Porchiett, Marcelo Lisandro Micheloud, Juan Francisco Eurytrema Diagnóstico Sedimentación Diagnosis Sedimentation Cattle Sheep Ganado Bovino Ovinos Coprology Eurytrematosis is a disease caused by flukes of the genus Eurytrema. These parasites infect the pancreatic ducts of a wide variety of species, including cattle, sheep and humans. Diagnosing eurytrematosis through the analysis of faecal samples can be difficult because most of the available techniques are considered of low sensitivity. In this context, a modification of the Dennis, Stone and Swanson technique (Belem Sedimentation Technique, BST) was previously developed to increase the probability of detecting infected animals; nevertheless, the values of eggs per gram obtained using the modified technique are generally low. We proposed a modification of the this technique (MBST), to increase the sensitivity and detection rate of infected animals. The objective of this work was to describe MBST and compare it with BST. Faecal samples of 212 clinically healthy animals (174 from cattle and 38 from sheep) from 20 farms were taken by the intra-rectal route and stored at 4°C. The samples were processed using BST and MBST. Positive samples amounted to 55 (25.9%) using BST and 121 (57.1%) using MBST. In the simples from cattle, 52 (29.8%) and 107 (61.4%) were positive in BST and MBST, respectively. In sheep, three (7.8%) and 14 (36.8%) positive samples were obtained in BST and MBST, respectively.The results obtained using the two methods were significantly different, indicating a lack of agreement between their findings. The results suggest that MBST is a more sensitive method to detect Eurytrema spp. eggs in faeces than BST. Instituto de Investigación Animal del Chaco Semiárido Fil: Olmos, Leandro Hipólito. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Investigación en Salud Animal; Argentina Fil: Olmos, Leandro Hipolito. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina Fil: Pantiu, Andrea Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Montecarlo; Argentina Fil: Avellaneda Cáceres, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Avellaneda Cáceres, Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Investigación en Salud Animal; Argentina Fil: Avellaneda Cáceres, Agustín. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina Fil: Valencia, P.N. Instituto San Cayetano N° 8092. Tecnicatura Superior en Laboratorio; Argentina Fil: Cayo, P.N. Instituto San Cayetano N° 8092. Tecnicatura Superior en Laboratorio; Argentina Fil: Signorini Porchiett, Marcelo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Signorini Porchiett, Marcelo Lisandro. Instituto Nacional de Tecnología Agropecuaria (INTA).Estación Experimental Agropecuaria Rafaela; Argentina Fil: Micheloud, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación Animal del Chaco Semiárido. Área de Investigación en Salud Animal; Argentina Fil: Micheloud, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Micheloud, Juan Francisco. Universidad Católica de Salta. Facultad de Ciencias Agrarias y Veterinarias; Argentina 2022-07-29T10:38:39Z 2022-07-29T10:38:39Z 2022-07-27 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12436 https://www.cambridge.org/core/journals/journal-of-helminthology/article/abs/comparison-of-two-coprological-methods-for-the-diagnosis-of-eurytrema-ssp-in-cattle-and-sheep/F240BADD6A4FC46156DFA71B9ADACBBC 0022-149X 1475-2697 (online) https://doi.org/10.1017/S0022149X22000414 eng info:eu-repograntAgreement/INTA/2019-PEM-E5-I702-001/2019-PEM-E5-I702-001/AR./Caracterización de la euritremosis en rumiantes en Misiones – Aportes para su control info:eu-repo/semantics/restrictedAccess application/pdf Cambridge University Press Journal of Helminthology 96 : e53 (2022) |
| spellingShingle | Eurytrema Diagnóstico Sedimentación Diagnosis Sedimentation Cattle Sheep Ganado Bovino Ovinos Coprology Olmos, Leandro Hipolito Pantiu, Andrea Julia Avellaneda Cáceres, Agustín Valencia, P.N. Cayo, P.N. Signorini Porchiett, Marcelo Lisandro Micheloud, Juan Francisco Comparison of two coprological methods for the diagnosis of Eurytrema ssp. in cattle and sheep |
| title | Comparison of two coprological methods for the diagnosis of Eurytrema ssp. in cattle and sheep |
| title_full | Comparison of two coprological methods for the diagnosis of Eurytrema ssp. in cattle and sheep |
| title_fullStr | Comparison of two coprological methods for the diagnosis of Eurytrema ssp. in cattle and sheep |
| title_full_unstemmed | Comparison of two coprological methods for the diagnosis of Eurytrema ssp. in cattle and sheep |
| title_short | Comparison of two coprological methods for the diagnosis of Eurytrema ssp. in cattle and sheep |
| title_sort | comparison of two coprological methods for the diagnosis of eurytrema ssp in cattle and sheep |
| topic | Eurytrema Diagnóstico Sedimentación Diagnosis Sedimentation Cattle Sheep Ganado Bovino Ovinos Coprology |
| url | http://hdl.handle.net/20.500.12123/12436 https://www.cambridge.org/core/journals/journal-of-helminthology/article/abs/comparison-of-two-coprological-methods-for-the-diagnosis-of-eurytrema-ssp-in-cattle-and-sheep/F240BADD6A4FC46156DFA71B9ADACBBC https://doi.org/10.1017/S0022149X22000414 |
| work_keys_str_mv | AT olmosleandrohipolito comparisonoftwocoprologicalmethodsforthediagnosisofeurytremasspincattleandsheep AT pantiuandreajulia comparisonoftwocoprologicalmethodsforthediagnosisofeurytremasspincattleandsheep AT avellanedacaceresagustin comparisonoftwocoprologicalmethodsforthediagnosisofeurytremasspincattleandsheep AT valenciapn comparisonoftwocoprologicalmethodsforthediagnosisofeurytremasspincattleandsheep AT cayopn comparisonoftwocoprologicalmethodsforthediagnosisofeurytremasspincattleandsheep AT signoriniporchiettmarcelolisandro comparisonoftwocoprologicalmethodsforthediagnosisofeurytremasspincattleandsheep AT micheloudjuanfrancisco comparisonoftwocoprologicalmethodsforthediagnosisofeurytremasspincattleandsheep |