Comparison of two coprological methods for the diagnosis of Eurytrema ssp. in cattle and sheep
Eurytrematosis is a disease caused by flukes of the genus Eurytrema. These parasites infect the pancreatic ducts of a wide variety of species, including cattle, sheep and humans. Diagnosing eurytrematosis through the analysis of faecal samples can be difficult because most of the available techni...
| Main Authors: | , , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
Cambridge University Press
2022
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/12436 https://www.cambridge.org/core/journals/journal-of-helminthology/article/abs/comparison-of-two-coprological-methods-for-the-diagnosis-of-eurytrema-ssp-in-cattle-and-sheep/F240BADD6A4FC46156DFA71B9ADACBBC https://doi.org/10.1017/S0022149X22000414 |
| Summary: | Eurytrematosis is a disease caused by flukes of the genus Eurytrema. These parasites infect the
pancreatic ducts of a wide variety of species, including cattle, sheep and humans. Diagnosing
eurytrematosis through the analysis of faecal samples can be difficult because most of the available
techniques are considered of low sensitivity. In this context, a modification of the Dennis,
Stone and Swanson technique (Belem Sedimentation Technique, BST) was previously developed
to increase the probability of detecting infected animals; nevertheless, the values of eggs per
gram obtained using the modified technique are generally low. We proposed a modification
of the this technique (MBST), to increase the sensitivity and detection rate of infected animals.
The objective of this work was to describe MBST and compare it with BST. Faecal samples of
212 clinically healthy animals (174 from cattle and 38 from sheep) from 20 farms were taken by
the intra-rectal route and stored at 4°C. The samples were processed using BST and MBST.
Positive samples amounted to 55 (25.9%) using BST and 121 (57.1%) using MBST. In the simples
from cattle, 52 (29.8%) and 107 (61.4%) were positive in BST and MBST, respectively. In
sheep, three (7.8%) and 14 (36.8%) positive samples were obtained in BST and MBST,
respectively.The results obtained using the two methods were significantly different, indicating
a lack of agreement between their findings. The results suggest that MBST is a more sensitive
method to detect Eurytrema spp. eggs in faeces than BST. |
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