Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins
Plant viruses encode movement proteins (MP) to facilitate cell-to-cell transport through plasmodesmata. In this study, using trans-complementation of a movement-defective turnip vein-clearing tobamovirus (TVCV) replicon, we show for the first time for cytorhabdoviruses (lettuce necrotic yellows viru...
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1235 http://www.sciencedirect.com/science/article/pii/S0042682215005097 https://doi.org/10.1016/j.virol.2015.11.028 |
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| author | Mann, Krin S. Bejerman, Nicolas Esteban Johnson, Karyn N. Dietzgen, Ralf Georg |
| author_browse | Bejerman, Nicolas Esteban Dietzgen, Ralf Georg Johnson, Karyn N. Mann, Krin S. |
| author_facet | Mann, Krin S. Bejerman, Nicolas Esteban Johnson, Karyn N. Dietzgen, Ralf Georg |
| author_sort | Mann, Krin S. |
| collection | INTA Digital |
| description | Plant viruses encode movement proteins (MP) to facilitate cell-to-cell transport through plasmodesmata. In this study, using trans-complementation of a movement-defective turnip vein-clearing tobamovirus (TVCV) replicon, we show for the first time for cytorhabdoviruses (lettuce necrotic yellows virus (LNYV) and alfalfa dwarf virus (ADV)) that their P3 proteins function as MP similar to the TVCV P30 protein. All three MP localized to plasmodesmata when ectopically expressed. In addition, we show that these MP belong to the 30K superfamily since movement was inhibited by mutation of an aspartic acid residue in the critical 30K-specific LxD/N50–70G motif. We also report that Nicotiana benthamiana microtubule-associated VOZ1-like transcriptional activator interacts with LNYV P3 and TVCV P30 but not with ADV P3 or any of the MP point mutants. This host protein, which is known to interact with P3 of sonchus yellow net nucleorhabdovirus, may be involved in aiding the cell-to-cell movement of LNYV and TVCV. |
| format | Artículo |
| id | INTA1235 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA12352019-03-22T13:58:55Z Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins Mann, Krin S. Bejerman, Nicolas Esteban Johnson, Karyn N. Dietzgen, Ralf Georg Enfermedades de las Plantas Virus de las Plantas Proteínas Plant Diseases Plant Viruses Proteins Cytorhabdovirus Plant viruses encode movement proteins (MP) to facilitate cell-to-cell transport through plasmodesmata. In this study, using trans-complementation of a movement-defective turnip vein-clearing tobamovirus (TVCV) replicon, we show for the first time for cytorhabdoviruses (lettuce necrotic yellows virus (LNYV) and alfalfa dwarf virus (ADV)) that their P3 proteins function as MP similar to the TVCV P30 protein. All three MP localized to plasmodesmata when ectopically expressed. In addition, we show that these MP belong to the 30K superfamily since movement was inhibited by mutation of an aspartic acid residue in the critical 30K-specific LxD/N50–70G motif. We also report that Nicotiana benthamiana microtubule-associated VOZ1-like transcriptional activator interacts with LNYV P3 and TVCV P30 but not with ADV P3 or any of the MP point mutants. This host protein, which is known to interact with P3 of sonchus yellow net nucleorhabdovirus, may be involved in aiding the cell-to-cell movement of LNYV and TVCV. Inst. Patología Vegetal Fil: Mann, Krin S. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia Fil: Bejerman, Nicolás Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Johnson, Karyn N. University of Queensland. School of Biological Sciences; Australia Fil: Dietzgen, Ralf Georg. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia 2017-09-18T13:19:02Z 2017-09-18T13:19:02Z 2016 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://hdl.handle.net/20.500.12123/1235 http://www.sciencedirect.com/science/article/pii/S0042682215005097 0042-6822 https://doi.org/10.1016/j.virol.2015.11.028 eng info:eu-repo/semantics/restrictedAccess application/pdf Virology 489 : 20-33. (February 2016) |
| spellingShingle | Enfermedades de las Plantas Virus de las Plantas Proteínas Plant Diseases Plant Viruses Proteins Cytorhabdovirus Mann, Krin S. Bejerman, Nicolas Esteban Johnson, Karyn N. Dietzgen, Ralf Georg Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins |
| title | Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins |
| title_full | Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins |
| title_fullStr | Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins |
| title_full_unstemmed | Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins |
| title_short | Cytorhabdovirus P3 genes encode 30K-like cell-to-cell movement proteins |
| title_sort | cytorhabdovirus p3 genes encode 30k like cell to cell movement proteins |
| topic | Enfermedades de las Plantas Virus de las Plantas Proteínas Plant Diseases Plant Viruses Proteins Cytorhabdovirus |
| url | http://hdl.handle.net/20.500.12123/1235 http://www.sciencedirect.com/science/article/pii/S0042682215005097 https://doi.org/10.1016/j.virol.2015.11.028 |
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