Differential mRNA accumulation upon early arabidopsis thaliana infection with ORMV and TMV-Cg Is associated with distinct endogenous small RNAs Level
Small RNAs (sRNAs) play important roles in plant development and host-pathogen interactions. Several studies have highlighted the relationship between viral infections, endogenous sRNA accumulation and transcriptional changes associated with symptoms. However, few studies have described a global...
| Main Authors: | , , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
| Published: |
2017
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| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/1232 http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0134719&type=printable https://doi.org/10.1371/journal.pone.0134719 |
| Summary: | Small RNAs (sRNAs) play important roles in plant development and host-pathogen interactions.
Several studies have highlighted the relationship between viral infections, endogenous
sRNA accumulation and transcriptional changes associated with symptoms.
However, few studies have described a global analysis of endogenous sRNAs by comparing
related viruses at early stages of infection, especially before viral accumulation reaches
systemic tissues. An sRNA high-throughput sequencing of Arabidopsis thaliana leaf samples
infected either with Oilseed rape mosaic virus (ORMV) or crucifer-infecting Tobacco
mosaic virus (TMV-Cg) with slightly different symptomatology at two early stages of infection
(2 and 4dpi) was performed. At early stages, both viral infections strongly alter the patterns
of several types of endogenous sRNA species in distal tissues with no virus
accumulation suggesting a systemic signaling process foregoing to virus spread. A correlation
between sRNAs derived from protein coding genes and the associated mRNA transcripts
was also detected, indicating that an unknown recursive mechanism is involved in a
regulatory circuit encompassing this sRNA/mRNA equilibrium. This work represents the initial
step in uncovering how differential accumulation of endogenous sRNAs contributes to
explain the massive alteration of the transcriptome associated with plant-virus interactions |
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