Differential mRNA accumulation upon early arabidopsis thaliana infection with ORMV and TMV-Cg Is associated with distinct endogenous small RNAs Level
Small RNAs (sRNAs) play important roles in plant development and host-pathogen interactions. Several studies have highlighted the relationship between viral infections, endogenous sRNA accumulation and transcriptional changes associated with symptoms. However, few studies have described a global...
| Autores principales: | , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
2017
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/1232 http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0134719&type=printable https://doi.org/10.1371/journal.pone.0134719 |
| _version_ | 1855034769863081984 |
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| author | Zavallo, Diego Debat, Humberto Julio Conti, Gabriela Manacorda, Carlos Augusto Rodriguez, Maria Cecilia Asurmendi, Sebastian |
| author_browse | Asurmendi, Sebastian Conti, Gabriela Debat, Humberto Julio Manacorda, Carlos Augusto Rodriguez, Maria Cecilia Zavallo, Diego |
| author_facet | Zavallo, Diego Debat, Humberto Julio Conti, Gabriela Manacorda, Carlos Augusto Rodriguez, Maria Cecilia Asurmendi, Sebastian |
| author_sort | Zavallo, Diego |
| collection | INTA Digital |
| description | Small RNAs (sRNAs) play important roles in plant development and host-pathogen interactions.
Several studies have highlighted the relationship between viral infections, endogenous
sRNA accumulation and transcriptional changes associated with symptoms.
However, few studies have described a global analysis of endogenous sRNAs by comparing
related viruses at early stages of infection, especially before viral accumulation reaches
systemic tissues. An sRNA high-throughput sequencing of Arabidopsis thaliana leaf samples
infected either with Oilseed rape mosaic virus (ORMV) or crucifer-infecting Tobacco
mosaic virus (TMV-Cg) with slightly different symptomatology at two early stages of infection
(2 and 4dpi) was performed. At early stages, both viral infections strongly alter the patterns
of several types of endogenous sRNA species in distal tissues with no virus
accumulation suggesting a systemic signaling process foregoing to virus spread. A correlation
between sRNAs derived from protein coding genes and the associated mRNA transcripts
was also detected, indicating that an unknown recursive mechanism is involved in a
regulatory circuit encompassing this sRNA/mRNA equilibrium. This work represents the initial
step in uncovering how differential accumulation of endogenous sRNAs contributes to
explain the massive alteration of the transcriptome associated with plant-virus interactions |
| format | info:ar-repo/semantics/artículo |
| id | INTA1232 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA12322017-11-28T12:42:11Z Differential mRNA accumulation upon early arabidopsis thaliana infection with ORMV and TMV-Cg Is associated with distinct endogenous small RNAs Level Zavallo, Diego Debat, Humberto Julio Conti, Gabriela Manacorda, Carlos Augusto Rodriguez, Maria Cecilia Asurmendi, Sebastian Arabidopsis Thaliana ARN Virus de las Plantas Genética Genetics RNA Plant Viruses SRNA ORMV Small RNAs (sRNAs) play important roles in plant development and host-pathogen interactions. Several studies have highlighted the relationship between viral infections, endogenous sRNA accumulation and transcriptional changes associated with symptoms. However, few studies have described a global analysis of endogenous sRNAs by comparing related viruses at early stages of infection, especially before viral accumulation reaches systemic tissues. An sRNA high-throughput sequencing of Arabidopsis thaliana leaf samples infected either with Oilseed rape mosaic virus (ORMV) or crucifer-infecting Tobacco mosaic virus (TMV-Cg) with slightly different symptomatology at two early stages of infection (2 and 4dpi) was performed. At early stages, both viral infections strongly alter the patterns of several types of endogenous sRNA species in distal tissues with no virus accumulation suggesting a systemic signaling process foregoing to virus spread. A correlation between sRNAs derived from protein coding genes and the associated mRNA transcripts was also detected, indicating that an unknown recursive mechanism is involved in a regulatory circuit encompassing this sRNA/mRNA equilibrium. This work represents the initial step in uncovering how differential accumulation of endogenous sRNAs contributes to explain the massive alteration of the transcriptome associated with plant-virus interactions Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Debat, Humberto Julio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Manacorda, Carlos Augusto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Rodriguez, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-09-15T18:01:26Z 2017-09-15T18:01:26Z 2015-08-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1232 http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0134719&type=printable 1932-6203 https://doi.org/10.1371/journal.pone.0134719 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Plos one 10 (8) : e0134719. (2015). |
| spellingShingle | Arabidopsis Thaliana ARN Virus de las Plantas Genética Genetics RNA Plant Viruses SRNA ORMV Zavallo, Diego Debat, Humberto Julio Conti, Gabriela Manacorda, Carlos Augusto Rodriguez, Maria Cecilia Asurmendi, Sebastian Differential mRNA accumulation upon early arabidopsis thaliana infection with ORMV and TMV-Cg Is associated with distinct endogenous small RNAs Level |
| title | Differential mRNA accumulation upon early arabidopsis thaliana infection with ORMV and TMV-Cg Is associated with distinct endogenous small RNAs Level |
| title_full | Differential mRNA accumulation upon early arabidopsis thaliana infection with ORMV and TMV-Cg Is associated with distinct endogenous small RNAs Level |
| title_fullStr | Differential mRNA accumulation upon early arabidopsis thaliana infection with ORMV and TMV-Cg Is associated with distinct endogenous small RNAs Level |
| title_full_unstemmed | Differential mRNA accumulation upon early arabidopsis thaliana infection with ORMV and TMV-Cg Is associated with distinct endogenous small RNAs Level |
| title_short | Differential mRNA accumulation upon early arabidopsis thaliana infection with ORMV and TMV-Cg Is associated with distinct endogenous small RNAs Level |
| title_sort | differential mrna accumulation upon early arabidopsis thaliana infection with ormv and tmv cg is associated with distinct endogenous small rnas level |
| topic | Arabidopsis Thaliana ARN Virus de las Plantas Genética Genetics RNA Plant Viruses SRNA ORMV |
| url | http://hdl.handle.net/20.500.12123/1232 http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0134719&type=printable https://doi.org/10.1371/journal.pone.0134719 |
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