Biological, Molecular and Phiysiological Characterization of Four Soybean mosaic virus Isolates Present in Argentine Soybean Crops
Soybean mosaic virus (SMV) causes systemic infections in soybean plants, leading to chlorotic mosaic and producing significant yield losses. The virus is widely distributed in all soybean production areas in the world. In Argentina, three geographical isolates were identified: Marcos Juárez (MJ), Ma...
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| Format: | Artículo |
| Language: | Inglés |
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BioRxiv
2022
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| Online Access: | http://hdl.handle.net/20.500.12123/12258 https://www.biorxiv.org/content/10.1101/2021.06.10.447356v1 https://doi.org/10.1101/2021.06.10.447356 |
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| author | Maugeri Suarez, Mariel Rodriguez, Marianela Bejerman, Nicolas Esteban Laguna, Irma Graciela Rodriguez Pardina, Patricia |
| author_browse | Bejerman, Nicolas Esteban Laguna, Irma Graciela Maugeri Suarez, Mariel Rodriguez Pardina, Patricia Rodriguez, Marianela |
| author_facet | Maugeri Suarez, Mariel Rodriguez, Marianela Bejerman, Nicolas Esteban Laguna, Irma Graciela Rodriguez Pardina, Patricia |
| author_sort | Maugeri Suarez, Mariel |
| collection | INTA Digital |
| description | Soybean mosaic virus (SMV) causes systemic infections in soybean plants, leading to chlorotic mosaic and producing significant yield losses. The virus is widely distributed in all soybean production areas in the world. In Argentina, three geographical isolates were identified: Marcos Juárez (MJ), Manfredi (M), and North Western Argentina (NOA), and another isolate named “Planta Vinosa” (PV), which causes severe necrosis symptoms in some cultivars. Here, the biological, molecular and physiological characterization of these isolates was performed for the first time. Three of the four isolates showed a low genetic divergence in the evaluated genes (P1, CI and CP). Although SMV-NOA and SMV-PV had high homology at the sequence level, they showed wide differences in pathogenicity, seed mottling and the ability of transmission by seeds or aphids, as well as in physiological effects. SMV-NOA caused early alterations (before symptom appearance, BS) in ΦPSII and MDA content in leaves with respect to the other isolates. After the appearance of macroscopic symptoms (late symptoms, LS), SMV-M caused a significant increase in the content of MDA, total soluble sugars, and starch with respect to the other isolates. Thus, early alterations of ΦPSII and soluble sugars might have an impact on late viral symptoms. Likewise, SMV-MJ developed more severe symptoms in the susceptible Davis cultivar than in DM 4800. Therefore, our results show differences in genome, biological properties and physiological effects among SMV isolates as well as different interactions of SMV-MJ with two soybean cultivars. |
| format | Artículo |
| id | INTA12258 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | BioRxiv |
| publisherStr | BioRxiv |
| record_format | dspace |
| spelling | INTA122582025-12-30T11:27:36Z Biological, Molecular and Phiysiological Characterization of Four Soybean mosaic virus Isolates Present in Argentine Soybean Crops Maugeri Suarez, Mariel Rodriguez, Marianela Bejerman, Nicolas Esteban Laguna, Irma Graciela Rodriguez Pardina, Patricia Soybean Mosaic Virus Soybeans Plant Viruses Crops Argentina Soja Virus de las Plantas Virus Mosaico Soja Cultivos SMV Virus del Mosaico de la Soja Soybean mosaic virus (SMV) causes systemic infections in soybean plants, leading to chlorotic mosaic and producing significant yield losses. The virus is widely distributed in all soybean production areas in the world. In Argentina, three geographical isolates were identified: Marcos Juárez (MJ), Manfredi (M), and North Western Argentina (NOA), and another isolate named “Planta Vinosa” (PV), which causes severe necrosis symptoms in some cultivars. Here, the biological, molecular and physiological characterization of these isolates was performed for the first time. Three of the four isolates showed a low genetic divergence in the evaluated genes (P1, CI and CP). Although SMV-NOA and SMV-PV had high homology at the sequence level, they showed wide differences in pathogenicity, seed mottling and the ability of transmission by seeds or aphids, as well as in physiological effects. SMV-NOA caused early alterations (before symptom appearance, BS) in ΦPSII and MDA content in leaves with respect to the other isolates. After the appearance of macroscopic symptoms (late symptoms, LS), SMV-M caused a significant increase in the content of MDA, total soluble sugars, and starch with respect to the other isolates. Thus, early alterations of ΦPSII and soluble sugars might have an impact on late viral symptoms. Likewise, SMV-MJ developed more severe symptoms in the susceptible Davis cultivar than in DM 4800. Therefore, our results show differences in genome, biological properties and physiological effects among SMV isolates as well as different interactions of SMV-MJ with two soybean cultivars. Instituto de Patología Vegetal Fil: Maugeri Suarez, M. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales (FCEFyN); Argentina Fil: Rodriguez, Marianela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina Fil: Rodriguez, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA) ; Argentina Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Bejerman, Nicolas Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Laguna, Irma Graciela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina Fil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Rodriguez Pardina, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina 2022-07-06T11:45:48Z 2022-07-06T11:45:48Z 2021-06-10 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/submittedVersion http://hdl.handle.net/20.500.12123/12258 https://www.biorxiv.org/content/10.1101/2021.06.10.447356v1 2692-8205 https://doi.org/10.1101/2021.06.10.447356 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf BioRxiv BioRxiv : the preprint server for biology. (June 10, 2021) |
| spellingShingle | Soybean Mosaic Virus Soybeans Plant Viruses Crops Argentina Soja Virus de las Plantas Virus Mosaico Soja Cultivos SMV Virus del Mosaico de la Soja Maugeri Suarez, Mariel Rodriguez, Marianela Bejerman, Nicolas Esteban Laguna, Irma Graciela Rodriguez Pardina, Patricia Biological, Molecular and Phiysiological Characterization of Four Soybean mosaic virus Isolates Present in Argentine Soybean Crops |
| title | Biological, Molecular and Phiysiological Characterization of Four Soybean mosaic virus Isolates Present in Argentine Soybean Crops |
| title_full | Biological, Molecular and Phiysiological Characterization of Four Soybean mosaic virus Isolates Present in Argentine Soybean Crops |
| title_fullStr | Biological, Molecular and Phiysiological Characterization of Four Soybean mosaic virus Isolates Present in Argentine Soybean Crops |
| title_full_unstemmed | Biological, Molecular and Phiysiological Characterization of Four Soybean mosaic virus Isolates Present in Argentine Soybean Crops |
| title_short | Biological, Molecular and Phiysiological Characterization of Four Soybean mosaic virus Isolates Present in Argentine Soybean Crops |
| title_sort | biological molecular and phiysiological characterization of four soybean mosaic virus isolates present in argentine soybean crops |
| topic | Soybean Mosaic Virus Soybeans Plant Viruses Crops Argentina Soja Virus de las Plantas Virus Mosaico Soja Cultivos SMV Virus del Mosaico de la Soja |
| url | http://hdl.handle.net/20.500.12123/12258 https://www.biorxiv.org/content/10.1101/2021.06.10.447356v1 https://doi.org/10.1101/2021.06.10.447356 |
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