Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity
In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Un...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Frontiers Media
2022
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/12156 https://www.frontiersin.org/articles/10.3389/fmicb.2022.868575/full https://doi.org/10.3389/fmicb.2022.868575 |
| _version_ | 1855484921279152128 |
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| author | Garcia Guizzo, Melina Tirloni, Lucas Gonzalez, Sergio Alberto Farber, Marisa Diana Braz, Glória Parizi, Luís Fernando Dedavid e Silva, Lucas Andre Da Silva Vaz, Itabajara Oliveira, Pedro L. |
| author_browse | Braz, Glória Da Silva Vaz, Itabajara Dedavid e Silva, Lucas Andre Farber, Marisa Diana Garcia Guizzo, Melina Gonzalez, Sergio Alberto Oliveira, Pedro L. Parizi, Luís Fernando Tirloni, Lucas |
| author_facet | Garcia Guizzo, Melina Tirloni, Lucas Gonzalez, Sergio Alberto Farber, Marisa Diana Braz, Glória Parizi, Luís Fernando Dedavid e Silva, Lucas Andre Da Silva Vaz, Itabajara Oliveira, Pedro L. |
| author_sort | Garcia Guizzo, Melina |
| collection | INTA Digital |
| description | In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Understanding the molecular basis of the interaction between ticks and their mutualist endosymbionts may help to develop control methods based on microbiome manipulation. Previously, we showed that Rhipicephalus microplus larvae with reduced levels of Coxiella endosymbiont of R. microplus (CERM) were arrested at the metanymph life stage (partially engorged nymph) and did not molt into adults. In this study, we performed a transcriptomic differential analysis of the R. microplus metanymph in the presence and absence of its mutualist endosymbiont. The lack of CERM resulted in an altered expression profile of transcripts from several functional categories. Gene products such as DA-P36, protease inhibitors, metalloproteases, and evasins, which are involved in blood feeding capacity, were underexpressed in CERM-free metanymphs. Disregulation in genes related to extracellular matrix remodeling was also observed in the absence of the symbiont. Taken together, the observed alterations in gene expression may explain the blockage of development at the metanymph stage and reveal a novel physiological aspect of the symbiont-tick-vertebrate host interaction. |
| format | Artículo |
| id | INTA12156 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | INTA121562022-06-24T10:36:49Z Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity Garcia Guizzo, Melina Tirloni, Lucas Gonzalez, Sergio Alberto Farber, Marisa Diana Braz, Glória Parizi, Luís Fernando Dedavid e Silva, Lucas Andre Da Silva Vaz, Itabajara Oliveira, Pedro L. Coxiella Simbiontico Transcriptomas Microbiomas Sanidad Animal Symbionts Rhipicephalus Transcriptome Microbiomes Animal Health Rhipicephalus microplus In the past decade, metagenomics studies exploring tick microbiota have revealed widespread interactions between bacteria and arthropods, including symbiotic interactions. Functional studies showed that obligate endosymbionts contribute to tick biology, affecting reproductive fitness and molting. Understanding the molecular basis of the interaction between ticks and their mutualist endosymbionts may help to develop control methods based on microbiome manipulation. Previously, we showed that Rhipicephalus microplus larvae with reduced levels of Coxiella endosymbiont of R. microplus (CERM) were arrested at the metanymph life stage (partially engorged nymph) and did not molt into adults. In this study, we performed a transcriptomic differential analysis of the R. microplus metanymph in the presence and absence of its mutualist endosymbiont. The lack of CERM resulted in an altered expression profile of transcripts from several functional categories. Gene products such as DA-P36, protease inhibitors, metalloproteases, and evasins, which are involved in blood feeding capacity, were underexpressed in CERM-free metanymphs. Disregulation in genes related to extracellular matrix remodeling was also observed in the absence of the symbiont. Taken together, the observed alterations in gene expression may explain the blockage of development at the metanymph stage and reveal a novel physiological aspect of the symbiont-tick-vertebrate host interaction. Instituto de Biotecnología Fil: Garcia Guizzo, Melina. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Vector Biology Section; Estados Unidos Fil: Garcia Guizzo, Melina. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil Fil: Tirloni, Lucas. National Institute of Allergy and Infectious Diseases. Rocky Mountain Laboratories. Laboratory of Bacteriology. Tick-Pathogen Transmission Unit; Estados Unidos Fil: Gonzalez, Sergio Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnologia y Biologia Molecular; Argentina Fil: Gonzalez, Sergio Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Farber, Marisa Diana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnologia y Biologia Molecular; Argentina Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Braz, Glória. Universidade Federal do Rio de Janeiro. Instituto de Química; Brasil Fil: Parizi, Luís Fernando. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil Fil: Dedavid e Silva, Lucas Andre. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil Fil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul. Centro de Biotecnologia; Brasil Fil: Da Silva Vaz, Itabajara. Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária; Brasil Fil: Da Silva Vaz, Itabajara. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil Fil: Oliveira, Pedro L. Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis; Brasil Fil: Oliveira, Pedro L. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular; Brasil 2022-06-24T10:31:46Z 2022-06-24T10:31:46Z 2022-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/12156 https://www.frontiersin.org/articles/10.3389/fmicb.2022.868575/full 1664-302X https://doi.org/10.3389/fmicb.2022.868575 eng info:eu-repograntAgreement/INTA/PNBIO-1131043/AR./Bioinformática y Estadística Genómica. info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Frontiers Media Frontiers in Microbiology 13 : 868575 (Mayo 2022) |
| spellingShingle | Coxiella Simbiontico Transcriptomas Microbiomas Sanidad Animal Symbionts Rhipicephalus Transcriptome Microbiomes Animal Health Rhipicephalus microplus Garcia Guizzo, Melina Tirloni, Lucas Gonzalez, Sergio Alberto Farber, Marisa Diana Braz, Glória Parizi, Luís Fernando Dedavid e Silva, Lucas Andre Da Silva Vaz, Itabajara Oliveira, Pedro L. Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity |
| title | Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity |
| title_full | Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity |
| title_fullStr | Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity |
| title_full_unstemmed | Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity |
| title_short | Coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity |
| title_sort | coxiella endosymbiont of rhipicephalus microplus modulates tick physiology with a major impact in blood feeding capacity |
| topic | Coxiella Simbiontico Transcriptomas Microbiomas Sanidad Animal Symbionts Rhipicephalus Transcriptome Microbiomes Animal Health Rhipicephalus microplus |
| url | http://hdl.handle.net/20.500.12123/12156 https://www.frontiersin.org/articles/10.3389/fmicb.2022.868575/full https://doi.org/10.3389/fmicb.2022.868575 |
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