Prevalence and risk factors for shedding of Cryptosporidium spp. oocysts in dairy calves of Buenos Aires Province, Argentina

In order to determine the prevalence and risk factors for shedding of Cryptosporidium spp. in dairy calves, a cross-sectional study was carried out in the northeastern region of Buenos Aires Province, Argentina. Fecal samples from a total of 552 calves from 27 dairy herds were collected, along with...

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Autores principales: Garro, Carlos Javier, Morici, Gabriel Edgardo, Utges, María E., Tomazic, Mariela Luján, Schnittger, Leonhard
Formato: Artículo
Lenguaje:Inglés
Publicado: 2017
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/1214
http://www.sciencedirect.com/science/article/pii/S2405673115300234
https://doi.org/10.1016/j.parepi.2016.03.008
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author Garro, Carlos Javier
Morici, Gabriel Edgardo
Utges, María E.
Tomazic, Mariela Luján
Schnittger, Leonhard
author_browse Garro, Carlos Javier
Morici, Gabriel Edgardo
Schnittger, Leonhard
Tomazic, Mariela Luján
Utges, María E.
author_facet Garro, Carlos Javier
Morici, Gabriel Edgardo
Utges, María E.
Tomazic, Mariela Luján
Schnittger, Leonhard
author_sort Garro, Carlos Javier
collection INTA Digital
description In order to determine the prevalence and risk factors for shedding of Cryptosporidium spp. in dairy calves, a cross-sectional study was carried out in the northeastern region of Buenos Aires Province, Argentina. Fecal samples from a total of 552 calves from 27 dairy herds were collected, along with a questionnaire about management factors. Cryptosporidium spp. oocysts were detected by light microscopy using Kinyoun staining. Putative risk factors were tested for association using generalized linear mixed models (GLMMs). Oocyst shedding calves were found in 67% (CI95% = 49–84) of herds (corresponding to a true herd prevalence of 98%) and 16% (CI95% = 13–19) of calves (corresponding to a true calve prevalence of 8%). Withinherd prevalence ranged from 0 to 60%, with a median of 8%. Cryptosporidium spp. excretion was not associated with the type of liquid diet, gender, time the calf stayed with the dam after birth, use of antibiotics, blood presence in feces, and calving season. However, important highly significant risk factors of oocyst shedding of calves was an age of less or equal than 20 days (OR = 7.4; 95% CI95% = 3–16; P b 0.0001) and occurrence of diarrhea (OR = 5.5; 95% CI95% = 2–11; P b 0.0001). The observed association with young age strongly suggests an early exposure of neonatal calves to Cryptosporidium spp. oocysts in maternity pens and/ or an age-related susceptibility. Association with diarrhea suggests that Cryptosporidium spp. is an important enteropathogen primarily responsible for the cause of the observed diarrheal syndrome. Results demonstrate that Cryptosporidium spp. infection is widespread in the study region. Monitoring and control of this parasitic protozoan infection in dairy herds is recommended.
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spelling INTA12142017-12-22T13:09:05Z Prevalence and risk factors for shedding of Cryptosporidium spp. oocysts in dairy calves of Buenos Aires Province, Argentina Garro, Carlos Javier Morici, Gabriel Edgardo Utges, María E. Tomazic, Mariela Luján Schnittger, Leonhard Enfermedades de los Animales Cryptosporidium Ganado de Leche Factores de Riesgo Animal Diseases Dairy Cattle Risk Factors Cryptosporidium spp. Oocysts In order to determine the prevalence and risk factors for shedding of Cryptosporidium spp. in dairy calves, a cross-sectional study was carried out in the northeastern region of Buenos Aires Province, Argentina. Fecal samples from a total of 552 calves from 27 dairy herds were collected, along with a questionnaire about management factors. Cryptosporidium spp. oocysts were detected by light microscopy using Kinyoun staining. Putative risk factors were tested for association using generalized linear mixed models (GLMMs). Oocyst shedding calves were found in 67% (CI95% = 49–84) of herds (corresponding to a true herd prevalence of 98%) and 16% (CI95% = 13–19) of calves (corresponding to a true calve prevalence of 8%). Withinherd prevalence ranged from 0 to 60%, with a median of 8%. Cryptosporidium spp. excretion was not associated with the type of liquid diet, gender, time the calf stayed with the dam after birth, use of antibiotics, blood presence in feces, and calving season. However, important highly significant risk factors of oocyst shedding of calves was an age of less or equal than 20 days (OR = 7.4; 95% CI95% = 3–16; P b 0.0001) and occurrence of diarrhea (OR = 5.5; 95% CI95% = 2–11; P b 0.0001). The observed association with young age strongly suggests an early exposure of neonatal calves to Cryptosporidium spp. oocysts in maternity pens and/ or an age-related susceptibility. Association with diarrhea suggests that Cryptosporidium spp. is an important enteropathogen primarily responsible for the cause of the observed diarrheal syndrome. Results demonstrate that Cryptosporidium spp. infection is widespread in the study region. Monitoring and control of this parasitic protozoan infection in dairy herds is recommended. Inst. de Patobiología Fil: Garro, Carlos Javier. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología. Grupo de Epidemiología y Medicina Preventiva; Argentina Fil: Morici, Gabriel Edgardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología. Grupo de Parasitología; Argentina Fil: Utges, María E. Argentina. Ministerio de Salud. Centro Nacional de Diagnóstico e Investigación en Endemo-Epidemias, ANLIS “Dr. C. G. Malbrán”; Argentina Fil: Tomazic, Mariela Luján. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología. Grupo de Protozoos Patógenos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Schnittger, Leonhard. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología. Grupo de Protozoos Patógenos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2017-09-14T12:19:03Z 2017-09-14T12:19:03Z 2016 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1214 http://www.sciencedirect.com/science/article/pii/S2405673115300234 2405-6731 https://doi.org/10.1016/j.parepi.2016.03.008 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Buenos Aires (province) Parasite Epidemiology and Control 1 (2) : 36-41 (June 2016)
spellingShingle Enfermedades de los Animales
Cryptosporidium
Ganado de Leche
Factores de Riesgo
Animal Diseases
Dairy Cattle
Risk Factors
Cryptosporidium spp. Oocysts
Garro, Carlos Javier
Morici, Gabriel Edgardo
Utges, María E.
Tomazic, Mariela Luján
Schnittger, Leonhard
Prevalence and risk factors for shedding of Cryptosporidium spp. oocysts in dairy calves of Buenos Aires Province, Argentina
title Prevalence and risk factors for shedding of Cryptosporidium spp. oocysts in dairy calves of Buenos Aires Province, Argentina
title_full Prevalence and risk factors for shedding of Cryptosporidium spp. oocysts in dairy calves of Buenos Aires Province, Argentina
title_fullStr Prevalence and risk factors for shedding of Cryptosporidium spp. oocysts in dairy calves of Buenos Aires Province, Argentina
title_full_unstemmed Prevalence and risk factors for shedding of Cryptosporidium spp. oocysts in dairy calves of Buenos Aires Province, Argentina
title_short Prevalence and risk factors for shedding of Cryptosporidium spp. oocysts in dairy calves of Buenos Aires Province, Argentina
title_sort prevalence and risk factors for shedding of cryptosporidium spp oocysts in dairy calves of buenos aires province argentina
topic Enfermedades de los Animales
Cryptosporidium
Ganado de Leche
Factores de Riesgo
Animal Diseases
Dairy Cattle
Risk Factors
Cryptosporidium spp. Oocysts
url http://hdl.handle.net/20.500.12123/1214
http://www.sciencedirect.com/science/article/pii/S2405673115300234
https://doi.org/10.1016/j.parepi.2016.03.008
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