Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.]
Low molecular weight glutenin subunits (LMW-GS) encoded by the Glu-3 loci are known to contribute to wheat breadmaking quality. However, the specific effect of individual Glu-3 alleles is not well understood due to their complex protein banding patterns in SDS-PAGE and tight linkage with gliadins at...
| Main Authors: | , , , , |
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1201 http://www.sciencedirect.com/science/article/pii/S0733521015000211 |
| _version_ | 1855034759227375616 |
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| author | Bonafede, Marcos Tranquilli, Gabriela Pfluger, Laura Alicia Peña, Roberto Javier Dubcovsky, Jorge |
| author_browse | Bonafede, Marcos Dubcovsky, Jorge Peña, Roberto Javier Pfluger, Laura Alicia Tranquilli, Gabriela |
| author_facet | Bonafede, Marcos Tranquilli, Gabriela Pfluger, Laura Alicia Peña, Roberto Javier Dubcovsky, Jorge |
| author_sort | Bonafede, Marcos |
| collection | INTA Digital |
| description | Low molecular weight glutenin subunits (LMW-GS) encoded by the Glu-3 loci are known to contribute to wheat breadmaking quality. However, the specific effect of individual Glu-3 alleles is not well understood due to their complex protein banding patterns in SDS-PAGE and tight linkage with gliadins at the Gli-1 locus. Using DNA markers and a backcross program, we developed a set of nine near isogenic lines (NILs) including different Glu-A3/Gli-A1 or Glu-B3/Gli-B1 alleles in the genetic background of the Argentine variety ProINTA Imperial. The nine NILs and the control were evaluated in three different field trials in Argentina. Significant genotype-by-environment interactions were detected for most quality parameters indicating that the effects of the Glu-3/Gli-1 alleles are modulated by environmental differences. None of the NILs showed differences in total flour protein content, but relative changes in the abundance of particular classes of proteins cannot be ruled out. On average, the Glu-A3f, Glu-B3b, Glu-B3g and Glu-B3iMan alleles were associated with the highest values in gluten strength-related parameters, while Glu-A3e, Glu-B3a and Glu-B3iChu were consistently associated with weak gluten and low quality values. The value of different Glu-3/Gli-1 allele combinations to improve breadmaking quality is discussed. |
| format | info:ar-repo/semantics/artículo |
| id | INTA1201 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA12012018-02-16T15:45:22Z Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.] Bonafede, Marcos Tranquilli, Gabriela Pfluger, Laura Alicia Peña, Roberto Javier Dubcovsky, Jorge Trigo Triticum Aestivum Peso Molecular Gluteninas Variación Genética Alelos Panificación Molecular Weight Glutenins Genetic Variation Alleles Breadmaking Glu-3/Gli-1 Low molecular weight glutenin subunits (LMW-GS) encoded by the Glu-3 loci are known to contribute to wheat breadmaking quality. However, the specific effect of individual Glu-3 alleles is not well understood due to their complex protein banding patterns in SDS-PAGE and tight linkage with gliadins at the Gli-1 locus. Using DNA markers and a backcross program, we developed a set of nine near isogenic lines (NILs) including different Glu-A3/Gli-A1 or Glu-B3/Gli-B1 alleles in the genetic background of the Argentine variety ProINTA Imperial. The nine NILs and the control were evaluated in three different field trials in Argentina. Significant genotype-by-environment interactions were detected for most quality parameters indicating that the effects of the Glu-3/Gli-1 alleles are modulated by environmental differences. None of the NILs showed differences in total flour protein content, but relative changes in the abundance of particular classes of proteins cannot be ruled out. On average, the Glu-A3f, Glu-B3b, Glu-B3g and Glu-B3iMan alleles were associated with the highest values in gluten strength-related parameters, while Glu-A3e, Glu-B3a and Glu-B3iChu were consistently associated with weak gluten and low quality values. The value of different Glu-3/Gli-1 allele combinations to improve breadmaking quality is discussed. Fil: Bonafede, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Tranquilli, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Pfluger, Laura Alicia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina Fil: Peña, Roberto Javier. Centro Internacional de Mejoramiento de Maíz y Trigo; México Fil: Dubcovsky, Jorge. University of California. Department of Plant Sciences; Estados Unidos. Howard Hughes Medical Institute; Estados Unidos 2017-09-12T17:08:11Z 2017-09-12T17:08:11Z 2015-03 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1201 http://www.sciencedirect.com/science/article/pii/S0733521015000211 0733-5210 eng info:eu-repo/semantics/restrictedAccess application/pdf Journal of cereal science 62 : 143-150. (March 2015) |
| spellingShingle | Trigo Triticum Aestivum Peso Molecular Gluteninas Variación Genética Alelos Panificación Molecular Weight Glutenins Genetic Variation Alleles Breadmaking Glu-3/Gli-1 Bonafede, Marcos Tranquilli, Gabriela Pfluger, Laura Alicia Peña, Roberto Javier Dubcovsky, Jorge Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.] |
| title | Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.] |
| title_full | Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.] |
| title_fullStr | Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.] |
| title_full_unstemmed | Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.] |
| title_short | Effect of allelic variation at the Glu-3 / Gli-1 loci on breadmaking quality parameters in hexaploid wheat [Triticum aestivum L.] |
| title_sort | effect of allelic variation at the glu 3 gli 1 loci on breadmaking quality parameters in hexaploid wheat triticum aestivum l |
| topic | Trigo Triticum Aestivum Peso Molecular Gluteninas Variación Genética Alelos Panificación Molecular Weight Glutenins Genetic Variation Alleles Breadmaking Glu-3/Gli-1 |
| url | http://hdl.handle.net/20.500.12123/1201 http://www.sciencedirect.com/science/article/pii/S0733521015000211 |
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