Replication and transmission features of two experimental vaccine candidates against bovine tuberculosis subcutaneously administrated in a murine model
Cattle vaccination is an attractive approach in compliance with control and eradication programs against Bovine Tuberculosis (bTB). Today, there is no anti bTB vaccine licensed. Two vaccine candidates, MbΔmce2 and MbΔmce2-phoP previously designed were evaluated in BALB/c mice, including the parental...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2022
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| Acceso en línea: | http://hdl.handle.net/20.500.12123/11933 https://www.sciencedirect.com/science/article/abs/pii/S1472979222000403 https://doi.org/10.1016/j.tube.2022.102203 |
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| author | Ferrara Muñiz, Ximena Garcia, Elizabeth Andrea Blanco, Federico Carlos Bigi, Fabiana Zumarraga, Martin Jose Cataldi, Angel Adrian Eirin, Maria Emilia |
| author_browse | Bigi, Fabiana Blanco, Federico Carlos Cataldi, Angel Adrian Eirin, Maria Emilia Ferrara Muñiz, Ximena Garcia, Elizabeth Andrea Zumarraga, Martin Jose |
| author_facet | Ferrara Muñiz, Ximena Garcia, Elizabeth Andrea Blanco, Federico Carlos Bigi, Fabiana Zumarraga, Martin Jose Cataldi, Angel Adrian Eirin, Maria Emilia |
| author_sort | Ferrara Muñiz, Ximena |
| collection | INTA Digital |
| description | Cattle vaccination is an attractive approach in compliance with control and eradication programs against Bovine Tuberculosis (bTB). Today, there is no anti bTB vaccine licensed. Two vaccine candidates, MbΔmce2 and MbΔmce2-phoP previously designed were evaluated in BALB/c mice, including the parental M. bovis NCTC10772 and a M. bovis hypervirulent Mb04-303 strains as controls. Sentinel mice (non-inoculated) cohoused with subcutaneous inoculated mice. Persistence, visible tuberculosis lesions (VTL) in lungs and spleens and bacillary load were investigated subcutaneously delivered at 60 and 90 days after inoculation (dpi) as well as their potential transmission to naïve mice.
While a 100% survival was observed at 90 dpi without VTL in all groups, transmission was not evidenced in the sentinels mice. Vaccine candidates and control strains were isolated from the spleen of all inoculated mice, while Mb04-303 was isolated from the lungs of one inoculated mouse. Vaccine candidate's attenuation considering survival, lung bacillary load and VTL was confirmed, administrated by the subcutaneous route. Future experiments are necessary to demonstrate whether the persistence of both mutants in the spleen, with low CFU, remains over time to increase the potential increasing risk of dissemination to organs and subsequent transmission to other animals by airborne or other routes. |
| format | Artículo |
| id | INTA11933 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2022 |
| publishDateRange | 2022 |
| publishDateSort | 2022 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA119332022-05-23T18:14:33Z Replication and transmission features of two experimental vaccine candidates against bovine tuberculosis subcutaneously administrated in a murine model Ferrara Muñiz, Ximena Garcia, Elizabeth Andrea Blanco, Federico Carlos Bigi, Fabiana Zumarraga, Martin Jose Cataldi, Angel Adrian Eirin, Maria Emilia Mycobacterium bovis Coadyuvantes de Vacunas Ratón Vaccine Adjuvants Tuberculosis Mice Murine Model Modelo Murino Cattle vaccination is an attractive approach in compliance with control and eradication programs against Bovine Tuberculosis (bTB). Today, there is no anti bTB vaccine licensed. Two vaccine candidates, MbΔmce2 and MbΔmce2-phoP previously designed were evaluated in BALB/c mice, including the parental M. bovis NCTC10772 and a M. bovis hypervirulent Mb04-303 strains as controls. Sentinel mice (non-inoculated) cohoused with subcutaneous inoculated mice. Persistence, visible tuberculosis lesions (VTL) in lungs and spleens and bacillary load were investigated subcutaneously delivered at 60 and 90 days after inoculation (dpi) as well as their potential transmission to naïve mice. While a 100% survival was observed at 90 dpi without VTL in all groups, transmission was not evidenced in the sentinels mice. Vaccine candidates and control strains were isolated from the spleen of all inoculated mice, while Mb04-303 was isolated from the lungs of one inoculated mouse. Vaccine candidate's attenuation considering survival, lung bacillary load and VTL was confirmed, administrated by the subcutaneous route. Future experiments are necessary to demonstrate whether the persistence of both mutants in the spleen, with low CFU, remains over time to increase the potential increasing risk of dissemination to organs and subsequent transmission to other animals by airborne or other routes. Instituto de Biotecnología Fil: Ferrara Muñiz, Ximena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ferrara Muñiz, Ximena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Garcia, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Eirin, Maria Emilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Eirin, Maria Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina 2022-05-23T18:09:51Z 2022-05-23T18:09:51Z 2022-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11933 https://www.sciencedirect.com/science/article/abs/pii/S1472979222000403 1472-9792 https://doi.org/10.1016/j.tube.2022.102203 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I102-001/2019-PD-E5-I102-001/AR./Desarrollo de vacunas y tecnologías para mejorar las estrategias profilácticas y terapéuticas de las enfermedades que afectan la producción animal y la salud pública info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Tuberculosis 134 : 102203 (Mayo 2022) |
| spellingShingle | Mycobacterium bovis Coadyuvantes de Vacunas Ratón Vaccine Adjuvants Tuberculosis Mice Murine Model Modelo Murino Ferrara Muñiz, Ximena Garcia, Elizabeth Andrea Blanco, Federico Carlos Bigi, Fabiana Zumarraga, Martin Jose Cataldi, Angel Adrian Eirin, Maria Emilia Replication and transmission features of two experimental vaccine candidates against bovine tuberculosis subcutaneously administrated in a murine model |
| title | Replication and transmission features of two experimental vaccine candidates against bovine tuberculosis subcutaneously administrated in a murine model |
| title_full | Replication and transmission features of two experimental vaccine candidates against bovine tuberculosis subcutaneously administrated in a murine model |
| title_fullStr | Replication and transmission features of two experimental vaccine candidates against bovine tuberculosis subcutaneously administrated in a murine model |
| title_full_unstemmed | Replication and transmission features of two experimental vaccine candidates against bovine tuberculosis subcutaneously administrated in a murine model |
| title_short | Replication and transmission features of two experimental vaccine candidates against bovine tuberculosis subcutaneously administrated in a murine model |
| title_sort | replication and transmission features of two experimental vaccine candidates against bovine tuberculosis subcutaneously administrated in a murine model |
| topic | Mycobacterium bovis Coadyuvantes de Vacunas Ratón Vaccine Adjuvants Tuberculosis Mice Murine Model Modelo Murino |
| url | http://hdl.handle.net/20.500.12123/11933 https://www.sciencedirect.com/science/article/abs/pii/S1472979222000403 https://doi.org/10.1016/j.tube.2022.102203 |
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