Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption
As clinical toxoplasmosis is not considered a problem in pigs, the main reason to implement a control strategy against Toxoplasma gondii (T. gondii) in this species is to reduce the establishment of T. gondii tissue cysts in pork, consequently reducing the risk of the parasite entering the human f...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Artículo |
| Language: | Inglés |
| Published: |
2017
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12123/1151 https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-015-0177-0 https://doi.org/10.1186/s13567-015-0177-0 |
| _version_ | 1855482837345501184 |
|---|---|
| author | Burrels, Alison Benavides-Silván, Julio Canton, German Jose Garcia, João L. Bartley, Paul M. Nath, Mintu Thomson, Jackie Chianini, Francesca Innes, Elisabeth A. Katze, Frank |
| author_browse | Bartley, Paul M. Benavides-Silván, Julio Burrels, Alison Canton, German Jose Chianini, Francesca Garcia, João L. Innes, Elisabeth A. Katze, Frank Nath, Mintu Thomson, Jackie |
| author_facet | Burrels, Alison Benavides-Silván, Julio Canton, German Jose Garcia, João L. Bartley, Paul M. Nath, Mintu Thomson, Jackie Chianini, Francesca Innes, Elisabeth A. Katze, Frank |
| author_sort | Burrels, Alison |
| collection | INTA Digital |
| description | As clinical toxoplasmosis is not considered a problem in pigs, the main reason to implement a control strategy against
Toxoplasma gondii (T. gondii) in this species is to reduce the establishment of T. gondii tissue cysts in pork, consequently
reducing the risk of the parasite entering the human food chain. Consumption of T. gondii tissue cysts from raw or
undercooked meat is one of the main sources of human infection, with infected pork being considered a high risk. This
study incorporates a mouse bioassay with molecular detection of T. gondii DNA to study the effectiveness of vaccination
(incomplete S48 strain) in its ability to reduce tissue cyst burden in pigs, following oocyst (M4 strain) challenge. Results
from the mouse bioassay show that 100% of mice which had received porcine tissues from vaccinated and challenged
pigs survived compared with 51.1% of mice which received tissues from non-vaccinated and challenged pigs. The
presence (or absence) of T. gondii DNA from individual mouse brains also confirmed these results. This indicates a
reduction in viable T. gondii tissue cysts within tissues from pigs which have been previously vaccinated with the S48
strain. In addition, the study demonstrated that the main predilection sites for the parasite were found to be brain and
highly vascular muscles (such as tongue, diaphragm, heart and masseter) of pigs, while meat cuts used as human food
such as chop, loin, left tricep and left semitendinosus, had a lower burden of T. gondii tissue cysts. These promising
results highlight the potential of S48 strain tachyzoites for reducing the number of T. gondii tissues cysts in pork and thus
improving food safety |
| format | Artículo |
| id | INTA1151 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA11512019-02-01T12:00:24Z Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption Burrels, Alison Benavides-Silván, Julio Canton, German Jose Garcia, João L. Bartley, Paul M. Nath, Mintu Thomson, Jackie Chianini, Francesca Innes, Elisabeth A. Katze, Frank Vacunación Vaccination Swine Animal Diseases Pork Cerdo Toxoplasma Gondii Enfermedades de los Animales Carne de Cerdo S48 Strain Cepa S48 As clinical toxoplasmosis is not considered a problem in pigs, the main reason to implement a control strategy against Toxoplasma gondii (T. gondii) in this species is to reduce the establishment of T. gondii tissue cysts in pork, consequently reducing the risk of the parasite entering the human food chain. Consumption of T. gondii tissue cysts from raw or undercooked meat is one of the main sources of human infection, with infected pork being considered a high risk. This study incorporates a mouse bioassay with molecular detection of T. gondii DNA to study the effectiveness of vaccination (incomplete S48 strain) in its ability to reduce tissue cyst burden in pigs, following oocyst (M4 strain) challenge. Results from the mouse bioassay show that 100% of mice which had received porcine tissues from vaccinated and challenged pigs survived compared with 51.1% of mice which received tissues from non-vaccinated and challenged pigs. The presence (or absence) of T. gondii DNA from individual mouse brains also confirmed these results. This indicates a reduction in viable T. gondii tissue cysts within tissues from pigs which have been previously vaccinated with the S48 strain. In addition, the study demonstrated that the main predilection sites for the parasite were found to be brain and highly vascular muscles (such as tongue, diaphragm, heart and masseter) of pigs, while meat cuts used as human food such as chop, loin, left tricep and left semitendinosus, had a lower burden of T. gondii tissue cysts. These promising results highlight the potential of S48 strain tachyzoites for reducing the number of T. gondii tissues cysts in pork and thus improving food safety Fil: Burrels, Alison. Moredun Research Institute. Pentlands Science Park; Reino Unido Fil: Benavides-Silván, Julio. Moredun Research Institute. Pentlands Science Park; Reino Unido. Instituto de Ganadería de Montaña; España Fil: Canton, German José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Moredun Research Institute. Pentlands Science Park; Reino Unido Fil: Garcia, João L. Moredun Research Institute. Pentlands Science Park; Reino Unido. Universidade Estadual de Londrina. Departamento de Medicina Veterinária Preventiva; Brasil Fil: Bartley, Paul M. Moredun Research Institute. Pentlands Science Park; Reino Unido Fil: Nath, Mintu. Biomathematics & Statistics Scotland; Reino Unido Fil: Thomson, Jackie. Moredun Research Institute. Pentlands Science Park; Reino Unido Fil: Chianini, Francesca. Moredun Research Institute. Pentlands Science Park; Reino Unido Fil: Innes, Elisabeth A. Moredun Research Institute. Pentlands Science Park; Reino Unido Fil: Katze, Frank. Moredun Research Institute. Pentlands Science Park; Reino Unido 2017-09-06T15:05:29Z 2017-09-06T15:05:29Z 2015 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1151 https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-015-0177-0 1297-9716 https://doi.org/10.1186/s13567-015-0177-0 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Veterinary research 46 (1) : 1-12. (2015) |
| spellingShingle | Vacunación Vaccination Swine Animal Diseases Pork Cerdo Toxoplasma Gondii Enfermedades de los Animales Carne de Cerdo S48 Strain Cepa S48 Burrels, Alison Benavides-Silván, Julio Canton, German Jose Garcia, João L. Bartley, Paul M. Nath, Mintu Thomson, Jackie Chianini, Francesca Innes, Elisabeth A. Katze, Frank Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption |
| title | Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption |
| title_full | Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption |
| title_fullStr | Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption |
| title_full_unstemmed | Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption |
| title_short | Vaccination of pigs with the S48 strain of Toxoplasma gondii – safer meat for human consumption |
| title_sort | vaccination of pigs with the s48 strain of toxoplasma gondii safer meat for human consumption |
| topic | Vacunación Vaccination Swine Animal Diseases Pork Cerdo Toxoplasma Gondii Enfermedades de los Animales Carne de Cerdo S48 Strain Cepa S48 |
| url | http://hdl.handle.net/20.500.12123/1151 https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-015-0177-0 https://doi.org/10.1186/s13567-015-0177-0 |
| work_keys_str_mv | AT burrelsalison vaccinationofpigswiththes48strainoftoxoplasmagondiisafermeatforhumanconsumption AT benavidessilvanjulio vaccinationofpigswiththes48strainoftoxoplasmagondiisafermeatforhumanconsumption AT cantongermanjose vaccinationofpigswiththes48strainoftoxoplasmagondiisafermeatforhumanconsumption AT garciajoaol vaccinationofpigswiththes48strainoftoxoplasmagondiisafermeatforhumanconsumption AT bartleypaulm vaccinationofpigswiththes48strainoftoxoplasmagondiisafermeatforhumanconsumption AT nathmintu vaccinationofpigswiththes48strainoftoxoplasmagondiisafermeatforhumanconsumption AT thomsonjackie vaccinationofpigswiththes48strainoftoxoplasmagondiisafermeatforhumanconsumption AT chianinifrancesca vaccinationofpigswiththes48strainoftoxoplasmagondiisafermeatforhumanconsumption AT inneselisabetha vaccinationofpigswiththes48strainoftoxoplasmagondiisafermeatforhumanconsumption AT katzefrank vaccinationofpigswiththes48strainoftoxoplasmagondiisafermeatforhumanconsumption |