Mycobacterial MCE proteins as transporters that control lipid homeostasis of the cell wall

Mammalian cell entry (mce) genes are not only present in genomes of pathogenic mycobacteria, including Mycobacterium tuberculosis (the causative agent of tuberculosis), but also in saprophytic and opportunistic mycobacterial species. MCE are conserved cell-wall proteins encoded by mce operons, which...

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Autores principales: Klepp, Laura Ines, Sabio Y Garcia, Julia Veronica, Bigi, Fabiana
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: Elsevier 2022
Materias:
Acceso en línea:http://hdl.handle.net/20.500.12123/11116
https://www.sciencedirect.com/science/article/abs/pii/S1472979221001128
https://doi.org/10.1016/j.tube.2021.102162
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author Klepp, Laura Ines
Sabio Y Garcia, Julia Veronica
Bigi, Fabiana
author_browse Bigi, Fabiana
Klepp, Laura Ines
Sabio Y Garcia, Julia Veronica
author_facet Klepp, Laura Ines
Sabio Y Garcia, Julia Veronica
Bigi, Fabiana
author_sort Klepp, Laura Ines
collection INTA Digital
description Mammalian cell entry (mce) genes are not only present in genomes of pathogenic mycobacteria, including Mycobacterium tuberculosis (the causative agent of tuberculosis), but also in saprophytic and opportunistic mycobacterial species. MCE are conserved cell-wall proteins encoded by mce operons, which maintain an identical structure in all mycobacteria: two yrbE genes (A and B) followed by six mce genes (A, B, C, D, E and F). Although these proteins are known to participate in the virulence of pathogenic mycobacteria, the presence of the operons in nonpathogenic mycobacteria and other bacteria indicates that they play another role apart from host cell invasion. In this respect, more recent studies suggest that they are functionally similar to ABC transporters and form part of lipid transporters in Actinobacteria. To date, most reviews on mce operons in the literature discuss their role in virulence. However, according to data from transcriptional studies, mce genes, particularly the mce1 and mce4 operons, modify their expression according to the carbon source and upon hypoxia, starvation, surface stress and oxidative stress; which suggests a role of MCE proteins in the response of Mycobacteria to external stressors. In addition to these data, this review also summarizes the studies demonstrating the role of MCE proteins as lipid transporters as well as the relevance of their transport function in the interaction of pathogenic Mycobacteria with the hosts. Altogether, the evidence to date would indicate that MCE proteins participate in the response to the stress conditions that mycobacteria encounter during infection, by participating in the cell wall remodelling and possibly contributing to lipid homeostasis.
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spelling INTA111162022-01-13T13:20:15Z Mycobacterial MCE proteins as transporters that control lipid homeostasis of the cell wall Klepp, Laura Ines Sabio Y Garcia, Julia Veronica Bigi, Fabiana Mycobacterium tuberculosis Pared Celular Lípidos Proteínas Homeostasis Cell Walls Lipids Proteins Mammalian cell entry (mce) genes are not only present in genomes of pathogenic mycobacteria, including Mycobacterium tuberculosis (the causative agent of tuberculosis), but also in saprophytic and opportunistic mycobacterial species. MCE are conserved cell-wall proteins encoded by mce operons, which maintain an identical structure in all mycobacteria: two yrbE genes (A and B) followed by six mce genes (A, B, C, D, E and F). Although these proteins are known to participate in the virulence of pathogenic mycobacteria, the presence of the operons in nonpathogenic mycobacteria and other bacteria indicates that they play another role apart from host cell invasion. In this respect, more recent studies suggest that they are functionally similar to ABC transporters and form part of lipid transporters in Actinobacteria. To date, most reviews on mce operons in the literature discuss their role in virulence. However, according to data from transcriptional studies, mce genes, particularly the mce1 and mce4 operons, modify their expression according to the carbon source and upon hypoxia, starvation, surface stress and oxidative stress; which suggests a role of MCE proteins in the response of Mycobacteria to external stressors. In addition to these data, this review also summarizes the studies demonstrating the role of MCE proteins as lipid transporters as well as the relevance of their transport function in the interaction of pathogenic Mycobacteria with the hosts. Altogether, the evidence to date would indicate that MCE proteins participate in the response to the stress conditions that mycobacteria encounter during infection, by participating in the cell wall remodelling and possibly contributing to lipid homeostasis. Instituto de Biotecnología Fil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Sabio Y Garcia, Julia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina Fil: Bigi, Fabiana. Consejo Nacional de investigaciones Científicas y Tecnológicas; Argentina 2022-01-13T13:18:07Z 2022-01-13T13:18:07Z 2022-01 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/11116 https://www.sciencedirect.com/science/article/abs/pii/S1472979221001128 1472-9792 https://doi.org/10.1016/j.tube.2021.102162 eng info:eu-repograntAgreement/INTA/2019-PD-E5-I103-001/2019-PD-E5-I103-001/AR./Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud pública info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001/AR./Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco “Una Salud” info:eu-repo/semantics/restrictedAccess application/pdf Elsevier Tuberculosis 132 : 102162 (January 2022)
spellingShingle Mycobacterium tuberculosis
Pared Celular
Lípidos
Proteínas
Homeostasis
Cell Walls
Lipids
Proteins
Klepp, Laura Ines
Sabio Y Garcia, Julia Veronica
Bigi, Fabiana
Mycobacterial MCE proteins as transporters that control lipid homeostasis of the cell wall
title Mycobacterial MCE proteins as transporters that control lipid homeostasis of the cell wall
title_full Mycobacterial MCE proteins as transporters that control lipid homeostasis of the cell wall
title_fullStr Mycobacterial MCE proteins as transporters that control lipid homeostasis of the cell wall
title_full_unstemmed Mycobacterial MCE proteins as transporters that control lipid homeostasis of the cell wall
title_short Mycobacterial MCE proteins as transporters that control lipid homeostasis of the cell wall
title_sort mycobacterial mce proteins as transporters that control lipid homeostasis of the cell wall
topic Mycobacterium tuberculosis
Pared Celular
Lípidos
Proteínas
Homeostasis
Cell Walls
Lipids
Proteins
url http://hdl.handle.net/20.500.12123/11116
https://www.sciencedirect.com/science/article/abs/pii/S1472979221001128
https://doi.org/10.1016/j.tube.2021.102162
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