Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle
The zoonotic enterohemorrhagic Escherichia coli (EHEC) O157: H7 bacterium causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS) in humans. Cattle are primary reservoirs and EHEC O157: H7; the bacteria predominately inhabit the colon and recto-anal junctions (RAJ). The early innat...
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| Format: | info:ar-repo/semantics/artículo |
| Language: | Inglés |
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SpringerNature
2021
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| Online Access: | http://hdl.handle.net/20.500.12123/10877 https://www.nature.com/articles/s41598-020-78752-x https://doi.org/10.1038/s41598-020-78752-x |
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| author | Larzabal, Mariano Marques Da Silva, Wanderson Multani, Anmol Vagnoni, Lucas Emilio Moore, Prando Dadin Marin, Maia Solange Riviere, Nahuel Agustin Delgado, Fernando Oscar Vilte, Daniel Alejandro Romero Victorica, Matias Tao, Ma Luo, Le Guan Talia, Paola Mónica Cataldi, Angel Adrian Cobo, Eduardo Rubén |
| author_browse | Cataldi, Angel Adrian Cobo, Eduardo Rubén Delgado, Fernando Oscar Larzabal, Mariano Luo, Le Guan Marin, Maia Solange Marques Da Silva, Wanderson Moore, Prando Dadin Multani, Anmol Riviere, Nahuel Agustin Romero Victorica, Matias Talia, Paola Mónica Tao, Ma Vagnoni, Lucas Emilio Vilte, Daniel Alejandro |
| author_facet | Larzabal, Mariano Marques Da Silva, Wanderson Multani, Anmol Vagnoni, Lucas Emilio Moore, Prando Dadin Marin, Maia Solange Riviere, Nahuel Agustin Delgado, Fernando Oscar Vilte, Daniel Alejandro Romero Victorica, Matias Tao, Ma Luo, Le Guan Talia, Paola Mónica Cataldi, Angel Adrian Cobo, Eduardo Rubén |
| author_sort | Larzabal, Mariano |
| collection | INTA Digital |
| description | The zoonotic enterohemorrhagic Escherichia coli (EHEC) O157: H7 bacterium causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS) in humans. Cattle are primary reservoirs and EHEC O157: H7; the bacteria predominately inhabit the colon and recto-anal junctions (RAJ). The early innate immune reactions in the infected gut are critical in the pathogenesis of EHEC O157: H7. In this study, calves orally inoculated with EHEC O157: H7 showed infiltration of neutrophils in the lamina propria of ileum and RAJ at 7 and 14 days post-infection. Infected calves had altered mucin layer and mast cell populations across small and large intestines. There were differential transcription expressions of key bovine β defensins, tracheal antimicrobial peptide (TAP) in the ileum, and lingual antimicrobial peptide (LAP) in RAJ. The main Gram-negative bacterial/LPS signaling Toll-Like receptor 4 (TLR4) was downregulated in RAJ. Intestinal infection with EHEC O157: H7 impacted the gut bacterial communities and influenced the relative abundance of Negativibacillus and Erysipelotrichaceae in mucosa-associated bacteria in the rectum. Thus, innate immunity in the gut of calves showed unique characteristics during infection with EHEC O157: H7, which occurred in the absence of major clinical manifestations but denoted an active immunological niche. |
| format | info:ar-repo/semantics/artículo |
| id | INTA10877 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | SpringerNature |
| publisherStr | SpringerNature |
| record_format | dspace |
| spelling | INTA108772021-12-09T18:11:41Z Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle Larzabal, Mariano Marques Da Silva, Wanderson Multani, Anmol Vagnoni, Lucas Emilio Moore, Prando Dadin Marin, Maia Solange Riviere, Nahuel Agustin Delgado, Fernando Oscar Vilte, Daniel Alejandro Romero Victorica, Matias Tao, Ma Luo, Le Guan Talia, Paola Mónica Cataldi, Angel Adrian Cobo, Eduardo Rubén Ganado Bovino Escherichia coli Inmunología Enfermedades de los Animales Enfermedades Intestinales Cattle Immunology Animal Diseases Intestinal Diseases The zoonotic enterohemorrhagic Escherichia coli (EHEC) O157: H7 bacterium causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS) in humans. Cattle are primary reservoirs and EHEC O157: H7; the bacteria predominately inhabit the colon and recto-anal junctions (RAJ). The early innate immune reactions in the infected gut are critical in the pathogenesis of EHEC O157: H7. In this study, calves orally inoculated with EHEC O157: H7 showed infiltration of neutrophils in the lamina propria of ileum and RAJ at 7 and 14 days post-infection. Infected calves had altered mucin layer and mast cell populations across small and large intestines. There were differential transcription expressions of key bovine β defensins, tracheal antimicrobial peptide (TAP) in the ileum, and lingual antimicrobial peptide (LAP) in RAJ. The main Gram-negative bacterial/LPS signaling Toll-Like receptor 4 (TLR4) was downregulated in RAJ. Intestinal infection with EHEC O157: H7 impacted the gut bacterial communities and influenced the relative abundance of Negativibacillus and Erysipelotrichaceae in mucosa-associated bacteria in the rectum. Thus, innate immunity in the gut of calves showed unique characteristics during infection with EHEC O157: H7, which occurred in the absence of major clinical manifestations but denoted an active immunological niche. Instituto de Biotecnología Fil: Larzabal, Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Larzabal, Mariano. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marques Da Silva, Wanderson. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular, Argentina Fil: Marques Da Silva, Wanderson. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Multani, Anmol. University of Calgary. Faculty Veterinary Medicine. Production Animal Health; Canadá Fil: Vagnoni, Lucas Emilio. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Vagnoni, Lucas Emilio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Moore, Prando Dadin. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Marin, Maia Solange. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina Fil: Marin, Maia Solange. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Riviere, Nahuel Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Riviere, Nahuel Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Delgado, Fernando Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Vilte, Daniel Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiologia; Argentina Fil: Vilte, Daniel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Romero Victorica, Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Romero Victorica, Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tao, Ma. University of Alberta. Department of Agricultural, Food and Nutritional Science; Canadá Fil: Tao, Ma. Chinese Academy of Agricultural Sciences. Feed Research Institute/Key Laboratory of Feed Biotechnology of the Ministry of Agriculture and Rural Affairs; China Fil: Luo, Le Guan. University of Alberta. Department of Agricultural, Food and Nutritional Science; Canadá Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Talia, Paola Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Cataldi, Angel Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cobo, Eduardo Rubén. University of Calgary. Faculty of Veterinary Medicine. Production Animal Health; Canadá 2021-12-09T18:05:36Z 2021-12-09T18:05:36Z 2020-12 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10877 https://www.nature.com/articles/s41598-020-78752-x 2045-2322 https://doi.org/10.1038/s41598-020-78752-x eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf SpringerNature Scientific Reports 10 : Article number: 21535 (2020) |
| spellingShingle | Ganado Bovino Escherichia coli Inmunología Enfermedades de los Animales Enfermedades Intestinales Cattle Immunology Animal Diseases Intestinal Diseases Larzabal, Mariano Marques Da Silva, Wanderson Multani, Anmol Vagnoni, Lucas Emilio Moore, Prando Dadin Marin, Maia Solange Riviere, Nahuel Agustin Delgado, Fernando Oscar Vilte, Daniel Alejandro Romero Victorica, Matias Tao, Ma Luo, Le Guan Talia, Paola Mónica Cataldi, Angel Adrian Cobo, Eduardo Rubén Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle |
| title | Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle |
| title_full | Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle |
| title_fullStr | Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle |
| title_full_unstemmed | Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle |
| title_short | Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle |
| title_sort | early immune innate hallmarks and microbiome changes across the gut during escherichia coli o157 h7 infection in cattle |
| topic | Ganado Bovino Escherichia coli Inmunología Enfermedades de los Animales Enfermedades Intestinales Cattle Immunology Animal Diseases Intestinal Diseases |
| url | http://hdl.handle.net/20.500.12123/10877 https://www.nature.com/articles/s41598-020-78752-x https://doi.org/10.1038/s41598-020-78752-x |
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