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Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle

The zoonotic enterohemorrhagic Escherichia coli (EHEC) O157: H7 bacterium causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS) in humans. Cattle are primary reservoirs and EHEC O157: H7; the bacteria predominately inhabit the colon and recto-anal junctions (RAJ). The early innat...

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Autores principales: Larzabal, Mariano, Marques Da Silva, Wanderson, Multani, Anmol, Vagnoni, Lucas Emilio, Moore, Prando Dadin, Marin, Maia Solange, Riviere, Nahuel Agustin, Delgado, Fernando Oscar, Vilte, Daniel Alejandro, Romero Victorica, Matias, Tao, Ma, Luo, Le Guan, Talia, Paola Mónica, Cataldi, Angel Adrian, Cobo, Eduardo Rubén
Formato: info:ar-repo/semantics/artículo
Lenguaje:Inglés
Publicado: SpringerNature 2021
Materias:
Ganado Bovino
Escherichia coli
Inmunología
Enfermedades de los Animales
Enfermedades Intestinales
Cattle
Immunology
Animal Diseases
Intestinal Diseases
Acceso en línea:http://hdl.handle.net/20.500.12123/10877
https://www.nature.com/articles/s41598-020-78752-x
https://doi.org/10.1038/s41598-020-78752-x
Sumario:The zoonotic enterohemorrhagic Escherichia coli (EHEC) O157: H7 bacterium causes diarrhea, hemorrhagic colitis, and hemolytic uremic syndrome (HUS) in humans. Cattle are primary reservoirs and EHEC O157: H7; the bacteria predominately inhabit the colon and recto-anal junctions (RAJ). The early innate immune reactions in the infected gut are critical in the pathogenesis of EHEC O157: H7. In this study, calves orally inoculated with EHEC O157: H7 showed infiltration of neutrophils in the lamina propria of ileum and RAJ at 7 and 14 days post-infection. Infected calves had altered mucin layer and mast cell populations across small and large intestines. There were differential transcription expressions of key bovine β defensins, tracheal antimicrobial peptide (TAP) in the ileum, and lingual antimicrobial peptide (LAP) in RAJ. The main Gram-negative bacterial/LPS signaling Toll-Like receptor 4 (TLR4) was downregulated in RAJ. Intestinal infection with EHEC O157: H7 impacted the gut bacterial communities and influenced the relative abundance of Negativibacillus and Erysipelotrichaceae in mucosa-associated bacteria in the rectum. Thus, innate immunity in the gut of calves showed unique characteristics during infection with EHEC O157: H7, which occurred in the absence of major clinical manifestations but denoted an active immunological niche.

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