Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome
Background: Metabolic syndrome (MetS) is a health issue with a high incidence in adult population. Using herbal medicines for the management of serious complications of MetS, such as dyslipidemia and hyperglycemia, is highly promising. An aqueous extract from Mulinum spinosum (M. spinosum), traditio...
| Autores principales: | , , , , , , , |
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| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/10852 https://www.sciencedirect.com/science/article/pii/S2667031321001512 https://doi.org/10.1016/j.phyplu.2021.100169 |
| _version_ | 1855036465308762112 |
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| author | Berruezo, Silvia Ferreyra, Susana Fontana, Ariel Paola, Matilde de Perez, María Belén Scapini, Celina Cremer, Cecilia Castro, Claudia |
| author_browse | Berruezo, Silvia Castro, Claudia Cremer, Cecilia Ferreyra, Susana Fontana, Ariel Paola, Matilde de Perez, María Belén Scapini, Celina |
| author_facet | Berruezo, Silvia Ferreyra, Susana Fontana, Ariel Paola, Matilde de Perez, María Belén Scapini, Celina Cremer, Cecilia Castro, Claudia |
| author_sort | Berruezo, Silvia |
| collection | INTA Digital |
| description | Background: Metabolic syndrome (MetS) is a health issue with a high incidence in adult population. Using herbal medicines for the management of serious complications of MetS, such as dyslipidemia and hyperglycemia, is highly promising. An aqueous extract from Mulinum spinosum (M. spinosum), traditionally used as hyperglycemic and anti-inflammatory, could have beneficial effects on the treatment of MetS.
Purpose: The present study was aimed to characterize the composition of M. spinosum roots decoction, and to evaluate antidiabetic, antilipemic and antioxidant effects in an animal model of MetS.
Study design and methods: M. spinosum roots extract was characterized using high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) method. Total Phenolic Content (TPC) was spectrophotometrically measured and the antioxidant capacity was determined by the oxygen radical absorbance capacity (ORAC) assay. To generate the MetS model, adult male C57BL/6 mice were fed with a 20% w/v fructose (Fru) in drinking water combined with 30% w/w high fat diet (HFD) over a period of 12 weeks. M. spinosum aqueous extracts (3 or 6 g/kg/day) were administered in drinking water together with Fru-HFD. At the end of the exposure period, mice were weighed and glucose tolerance test was determined. After sacrifice, adipose tissues were isolated and blood samples were collected to evaluate lipid profile, lipid peroxidation level (LPO) and ferric reducing antioxidant power (FRAP).
Results: The characterization of M. spinosum revealed the presence of caffeic acid, trans-resveratrol, kaempferol-3-glucoside, (-)-epicatechin, (-)-gallocatechin gallate, (-)-epigallocatechin and (+)-catechin. A high antioxidant capacity of the extract was revealed by ORAC test. Mice fed a Fru-HF diet and treated with M. spinosum, reduced diet-induced weight gain and significantly decreased mesenteric fat compared with Fru-HFD group (p<0.01). Post prandial glycaemia significantly diminished when mice were treated with M. spinosum, and also total cholesterol, LDL-cholesterol and triglycerides (TG) were reduced. Regarding the antioxidant effect of the consumption of M. spinosum, we found a decrease in plasmatic LPO, and a significant increase in plasmatic FRAP.
Conclusion: To our knowledge, the phenolic composition and the antioxidant capacity of M. spinosum roots decoction is reported for the first time. In addition, we provide evidence that M. spinosum root extract is a promising source of antioxidants able to alleviates Fru-HFD-induced metabolic alterations and slow the progression of MetS. |
| format | info:ar-repo/semantics/artículo |
| id | INTA10852 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | INTA108522021-12-06T11:54:10Z Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome Berruezo, Silvia Ferreyra, Susana Fontana, Ariel Paola, Matilde de Perez, María Belén Scapini, Celina Cremer, Cecilia Castro, Claudia Extractos Vegetales Control de Enfermedades Trastornos Metabólicos Obesidad Propiedades Antioxidantes Plant Extracts Disease Control Metabolic Disorders Obesity Antioxidant Properties Síndrome Metabólico Mulinum spinosum Metabolic Syndrome Background: Metabolic syndrome (MetS) is a health issue with a high incidence in adult population. Using herbal medicines for the management of serious complications of MetS, such as dyslipidemia and hyperglycemia, is highly promising. An aqueous extract from Mulinum spinosum (M. spinosum), traditionally used as hyperglycemic and anti-inflammatory, could have beneficial effects on the treatment of MetS. Purpose: The present study was aimed to characterize the composition of M. spinosum roots decoction, and to evaluate antidiabetic, antilipemic and antioxidant effects in an animal model of MetS. Study design and methods: M. spinosum roots extract was characterized using high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) method. Total Phenolic Content (TPC) was spectrophotometrically measured and the antioxidant capacity was determined by the oxygen radical absorbance capacity (ORAC) assay. To generate the MetS model, adult male C57BL/6 mice were fed with a 20% w/v fructose (Fru) in drinking water combined with 30% w/w high fat diet (HFD) over a period of 12 weeks. M. spinosum aqueous extracts (3 or 6 g/kg/day) were administered in drinking water together with Fru-HFD. At the end of the exposure period, mice were weighed and glucose tolerance test was determined. After sacrifice, adipose tissues were isolated and blood samples were collected to evaluate lipid profile, lipid peroxidation level (LPO) and ferric reducing antioxidant power (FRAP). Results: The characterization of M. spinosum revealed the presence of caffeic acid, trans-resveratrol, kaempferol-3-glucoside, (-)-epicatechin, (-)-gallocatechin gallate, (-)-epigallocatechin and (+)-catechin. A high antioxidant capacity of the extract was revealed by ORAC test. Mice fed a Fru-HF diet and treated with M. spinosum, reduced diet-induced weight gain and significantly decreased mesenteric fat compared with Fru-HFD group (p<0.01). Post prandial glycaemia significantly diminished when mice were treated with M. spinosum, and also total cholesterol, LDL-cholesterol and triglycerides (TG) were reduced. Regarding the antioxidant effect of the consumption of M. spinosum, we found a decrease in plasmatic LPO, and a significant increase in plasmatic FRAP. Conclusion: To our knowledge, the phenolic composition and the antioxidant capacity of M. spinosum roots decoction is reported for the first time. In addition, we provide evidence that M. spinosum root extract is a promising source of antioxidants able to alleviates Fru-HFD-induced metabolic alterations and slow the progression of MetS. EEA La Consulta Fil: Berruezo, Silvia. Universidad Nacional del Comahue. Facultad de Ciencias Médicas. Centro Interdisciplinario de Investigaciones Biomédicas y Clínicas; Argentina Fil: Ferreyra, Susana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Ferreyra, Susana. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Fontana, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Fontana, Ariel. Universidad Nacional de Cuyo. Facultad de Ciencias Agrarias. Instituto de Biología Agrícola de Mendoza; Argentina Fil: Paola, Matilde de. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Paola, Matilde de. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Bioquímica y Biotecnología; Argentina Fil: Pérez, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Pérez, María Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria La Consulta; Argentina Fil: Scapini, Celina. Universidad Nacional del Comahue. Facultad de Ciencias Médicas. Centro Interdisciplinario de Investigaciones Biomédicas y Clínicas; Argentina Fil: Cremer, Cecilia. Universidad Nacional del Comahue. Facultad de Ciencias Médicas. Centro Interdisciplinario de Investigaciones Biomédicas y Clínicas; Argentina Fil: Castro, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto de Medicina y Biología Experimental de Cuyo; Argentina Fil: Castro, Claudia. Universidad Nacional de Cuyo. Facultad de Ciencias Médicas. Instituto de Bioquímica y Biotecnología; Argentina 2021-12-06T11:52:22Z 2021-12-06T11:52:22Z 2022-02 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10852 https://www.sciencedirect.com/science/article/pii/S2667031321001512 2667-0313 https://doi.org/10.1016/j.phyplu.2021.100169 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Elsevier Phytomedicine Plus 2 (1) : 100169 (February 2022) |
| spellingShingle | Extractos Vegetales Control de Enfermedades Trastornos Metabólicos Obesidad Propiedades Antioxidantes Plant Extracts Disease Control Metabolic Disorders Obesity Antioxidant Properties Síndrome Metabólico Mulinum spinosum Metabolic Syndrome Berruezo, Silvia Ferreyra, Susana Fontana, Ariel Paola, Matilde de Perez, María Belén Scapini, Celina Cremer, Cecilia Castro, Claudia Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome |
| title | Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome |
| title_full | Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome |
| title_fullStr | Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome |
| title_full_unstemmed | Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome |
| title_short | Mulinum spinosum root extract, rich in antioxidant compounds, mitigates harmful effects in mice with diet-induced metabolic syndrome |
| title_sort | mulinum spinosum root extract rich in antioxidant compounds mitigates harmful effects in mice with diet induced metabolic syndrome |
| topic | Extractos Vegetales Control de Enfermedades Trastornos Metabólicos Obesidad Propiedades Antioxidantes Plant Extracts Disease Control Metabolic Disorders Obesity Antioxidant Properties Síndrome Metabólico Mulinum spinosum Metabolic Syndrome |
| url | http://hdl.handle.net/20.500.12123/10852 https://www.sciencedirect.com/science/article/pii/S2667031321001512 https://doi.org/10.1016/j.phyplu.2021.100169 |
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