Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction
One of the main distinguishing features of bacteria belonging to the Cellulomonas genus is their ability to secrete multiple polysaccharide degrading enzymes. However, their application in biomass deconstruction still constitutes a challenge. We addressed the optimisation of the xylanolytic activiti...
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | info:ar-repo/semantics/artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2021
|
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12123/10779 https://link.springer.com/article/10.1007%2Fs00253-021-11305-y https://doi.org/10.1007/s00253-021-11305-y |
| _version_ | 1855036451706634240 |
|---|---|
| author | Ontañon, Ornella Mailen Bedő, Soma Ghio, Silvina Garrido, Mercedes Maria Topalian, Juliana Jahola, Dóra Fehér, Anikó Valacco, Maria Pia Campos, Eleonora Fehér, Csaba |
| author_browse | Bedő, Soma Campos, Eleonora Fehér, Anikó Fehér, Csaba Garrido, Mercedes Maria Ghio, Silvina Jahola, Dóra Ontañon, Ornella Mailen Topalian, Juliana Valacco, Maria Pia |
| author_facet | Ontañon, Ornella Mailen Bedő, Soma Ghio, Silvina Garrido, Mercedes Maria Topalian, Juliana Jahola, Dóra Fehér, Anikó Valacco, Maria Pia Campos, Eleonora Fehér, Csaba |
| author_sort | Ontañon, Ornella Mailen |
| collection | INTA Digital |
| description | One of the main distinguishing features of bacteria belonging to the Cellulomonas genus is their ability to secrete multiple polysaccharide degrading enzymes. However, their application in biomass deconstruction still constitutes a challenge. We addressed the optimisation of the xylanolytic activities in extracellular enzymatic extracts of Cellulomonas sp. B6 and Cellulomonas fimi B-402 for their subsequent application in lignocellulosic biomass hydrolysis by culture in several substrates. As demonstrated by secretomic profiling, wheat bran and waste paper resulted to be suitable inducers for the secretion of xylanases of Cellulomonas sp. B6 and C. fimi B-402, respectively. Both strains showed high xylanolytic activity in culture supernatant although Cellulomonas sp. B6 was the most efficient xylanolytic strain. Upscaling from flasks to fermentation in a bench scale bioreactor resulted in equivalent production of extracellular xylanolytic enzymatic extracts and freeze drying was a successful method for concentration and conservation of the extracellular enzymes, retaining 80% activity. Moreover, enzymatic cocktails composed of combined extra and intracellular extracts effectively hydrolysed the hemicellulose fraction of extruded barley straw into xylose and xylooligosaccharides. |
| format | info:ar-repo/semantics/artículo |
| id | INTA10779 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | INTA107792021-11-15T16:22:42Z Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction Ontañon, Ornella Mailen Bedő, Soma Ghio, Silvina Garrido, Mercedes Maria Topalian, Juliana Jahola, Dóra Fehér, Anikó Valacco, Maria Pia Campos, Eleonora Fehér, Csaba Cellulomonas Lignocellulose Enzymatic Hydrolysis Biomass Lignocelulosa Hidrólisis Enzimática Biomasa Xilanasas Xylanases One of the main distinguishing features of bacteria belonging to the Cellulomonas genus is their ability to secrete multiple polysaccharide degrading enzymes. However, their application in biomass deconstruction still constitutes a challenge. We addressed the optimisation of the xylanolytic activities in extracellular enzymatic extracts of Cellulomonas sp. B6 and Cellulomonas fimi B-402 for their subsequent application in lignocellulosic biomass hydrolysis by culture in several substrates. As demonstrated by secretomic profiling, wheat bran and waste paper resulted to be suitable inducers for the secretion of xylanases of Cellulomonas sp. B6 and C. fimi B-402, respectively. Both strains showed high xylanolytic activity in culture supernatant although Cellulomonas sp. B6 was the most efficient xylanolytic strain. Upscaling from flasks to fermentation in a bench scale bioreactor resulted in equivalent production of extracellular xylanolytic enzymatic extracts and freeze drying was a successful method for concentration and conservation of the extracellular enzymes, retaining 80% activity. Moreover, enzymatic cocktails composed of combined extra and intracellular extracts effectively hydrolysed the hemicellulose fraction of extruded barley straw into xylose and xylooligosaccharides. Instituto de Biotecnología Fil: Ontañon, Ornella. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ontañon, Ornella. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bedő, Soma. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungría Fil: Ghio, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Ghio, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Garrido, Mercedes Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Garrido, Mercedes Maria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Topalian, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Fil: Topalian, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Jahola, Dóra. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungría Fil: Fehér, Anikó. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungría Fil: Valacco, Maria Pia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica. Centro de Estudios Químicos y Biológicos por Espectrometría de Masa; Argentina Fil: Valacco, Maria Pia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Campos, Eleonora. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fehér, Csaba. Budapest University of Technology and Economics. Faculty of Chemical Technology and Biotechnology. Department of Applied Biotechnology and Food Science. Biorefinery Research Group; Hungría 2021-11-15T16:19:12Z 2021-11-15T16:19:12Z 2021-05 info:ar-repo/semantics/artículo info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/10779 https://link.springer.com/article/10.1007%2Fs00253-021-11305-y 0175-7598 1432-0614 https://doi.org/10.1007/s00253-021-11305-y eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Springer Applied Microbiology and Biotechnology 105 : 4577-4588 (2021) |
| spellingShingle | Cellulomonas Lignocellulose Enzymatic Hydrolysis Biomass Lignocelulosa Hidrólisis Enzimática Biomasa Xilanasas Xylanases Ontañon, Ornella Mailen Bedő, Soma Ghio, Silvina Garrido, Mercedes Maria Topalian, Juliana Jahola, Dóra Fehér, Anikó Valacco, Maria Pia Campos, Eleonora Fehér, Csaba Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction |
| title | Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction |
| title_full | Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction |
| title_fullStr | Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction |
| title_full_unstemmed | Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction |
| title_short | Optimisation of xylanases production by two Cellulomonas strains and their use for biomass deconstruction |
| title_sort | optimisation of xylanases production by two cellulomonas strains and their use for biomass deconstruction |
| topic | Cellulomonas Lignocellulose Enzymatic Hydrolysis Biomass Lignocelulosa Hidrólisis Enzimática Biomasa Xilanasas Xylanases |
| url | http://hdl.handle.net/20.500.12123/10779 https://link.springer.com/article/10.1007%2Fs00253-021-11305-y https://doi.org/10.1007/s00253-021-11305-y |
| work_keys_str_mv | AT ontanonornellamailen optimisationofxylanasesproductionbytwocellulomonasstrainsandtheiruseforbiomassdeconstruction AT bedosoma optimisationofxylanasesproductionbytwocellulomonasstrainsandtheiruseforbiomassdeconstruction AT ghiosilvina optimisationofxylanasesproductionbytwocellulomonasstrainsandtheiruseforbiomassdeconstruction AT garridomercedesmaria optimisationofxylanasesproductionbytwocellulomonasstrainsandtheiruseforbiomassdeconstruction AT topalianjuliana optimisationofxylanasesproductionbytwocellulomonasstrainsandtheiruseforbiomassdeconstruction AT jaholadora optimisationofxylanasesproductionbytwocellulomonasstrainsandtheiruseforbiomassdeconstruction AT feheraniko optimisationofxylanasesproductionbytwocellulomonasstrainsandtheiruseforbiomassdeconstruction AT valaccomariapia optimisationofxylanasesproductionbytwocellulomonasstrainsandtheiruseforbiomassdeconstruction AT camposeleonora optimisationofxylanasesproductionbytwocellulomonasstrainsandtheiruseforbiomassdeconstruction AT fehercsaba optimisationofxylanasesproductionbytwocellulomonasstrainsandtheiruseforbiomassdeconstruction |