Cattle are a potential reservoir of bubaline herpesvirus 1 (BuHV1)
In the present work, controlled experimental infection and transmission studies in domestic cattle (Bos taurus) and water buffaloes (Bubalus bubalis) were carried out to study the in vivo behaviour of bubaline herpesvirus 1 (BuHV1). Two bovine and two buffalo calves were infected with BuHV1 (20287N...
| Main Authors: | , , , , , |
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| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
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2017
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| Online Access: | http://hdl.handle.net/20.500.12123/1068 http://vetrecordopen.bmj.com/content/vetreco/3/1/e000162.full.pdf http://dx.doi.org/10.1136/vetreco-2015-000162 |
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| author | Maidana, Silvina Soledad Delgado, Fernando Oscar Vagnoni, Lucas Emilio Mauroy, Axel Thiry, Etienne Romera, Sonia Alejandra |
| author_browse | Delgado, Fernando Oscar Maidana, Silvina Soledad Mauroy, Axel Romera, Sonia Alejandra Thiry, Etienne Vagnoni, Lucas Emilio |
| author_facet | Maidana, Silvina Soledad Delgado, Fernando Oscar Vagnoni, Lucas Emilio Mauroy, Axel Thiry, Etienne Romera, Sonia Alejandra |
| author_sort | Maidana, Silvina Soledad |
| collection | INTA Digital |
| description | In the present work, controlled experimental infection and transmission studies in domestic cattle (Bos taurus) and water buffaloes (Bubalus bubalis) were carried out to study the in vivo behaviour of bubaline herpesvirus 1 (BuHV1). Two bovine and two buffalo calves were infected with BuHV1 (20287N isolate) by intranasal aerosolisation. Two sentinel cattle did not receive the virus challenge, but were housed with infected buffaloes to evaluate horizontal transmission.
All experimentally inoculated animals showed viral infection and respiratory clinical signs. BuHV1 experimentally infected calves showed intermittent viral excretion between 2 days and 18 days postinfection (dpi) with a maximum titre of excretion of 106 TCID50/ml and moderate rhinitis between 2 dpi and 20 dpi. BuHV1 experimentally inoculated buffaloes showed mild respiratory signs, which consisted mainly of serous nasal secretions during the infection period. Sentinel calves showed mucosal specific IgG1 antibodies at seven days postcontact. Viral DNA was detected by PCR and sequencing in both buffaloes and sentinel calves, which could be associated with latency.
In conclusion, this study showed the susceptibility of cattle to BuHV1 after both experimental infection and contact with infected buffaloes. These data increase the scarce knowledge on the pathogenesis in natural host and the susceptibility of cattle to BuHV1 experimental infection. |
| format | info:eu-repo/semantics/article |
| id | INTA1068 |
| institution | Instituto Nacional de Tecnología Agropecuaria (INTA -Argentina) |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| record_format | dspace |
| spelling | INTA10682018-02-26T12:33:04Z Cattle are a potential reservoir of bubaline herpesvirus 1 (BuHV1) Maidana, Silvina Soledad Delgado, Fernando Oscar Vagnoni, Lucas Emilio Mauroy, Axel Thiry, Etienne Romera, Sonia Alejandra Enfermedades de los Animales Ganado Bovino Herpesviridae Búfalo de Agua Animal Diseases Cattle Water Buffaloes Herpesvirus In the present work, controlled experimental infection and transmission studies in domestic cattle (Bos taurus) and water buffaloes (Bubalus bubalis) were carried out to study the in vivo behaviour of bubaline herpesvirus 1 (BuHV1). Two bovine and two buffalo calves were infected with BuHV1 (20287N isolate) by intranasal aerosolisation. Two sentinel cattle did not receive the virus challenge, but were housed with infected buffaloes to evaluate horizontal transmission. All experimentally inoculated animals showed viral infection and respiratory clinical signs. BuHV1 experimentally infected calves showed intermittent viral excretion between 2 days and 18 days postinfection (dpi) with a maximum titre of excretion of 106 TCID50/ml and moderate rhinitis between 2 dpi and 20 dpi. BuHV1 experimentally inoculated buffaloes showed mild respiratory signs, which consisted mainly of serous nasal secretions during the infection period. Sentinel calves showed mucosal specific IgG1 antibodies at seven days postcontact. Viral DNA was detected by PCR and sequencing in both buffaloes and sentinel calves, which could be associated with latency. In conclusion, this study showed the susceptibility of cattle to BuHV1 after both experimental infection and contact with infected buffaloes. These data increase the scarce knowledge on the pathogenesis in natural host and the susceptibility of cattle to BuHV1 experimental infection. Inst.de Virología Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina Fil: Delgado, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Vagnoni, Lucas Emilio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Mauroy, Axel. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica Fil: Thiry, Etienne. University of Liège. Fundamental and Applied Research on Animal Health Center and Faculty of Veterinary Medicine. Veterinary Virology and Animal Viral Diseases; Bélgica Fil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Morón. Facultad de Ciencias Exactas, Químicas y Naturales. Cátedra de Inmunogenética; Argentina. Universidad del Salvador. Cátedra de Inmunología; Argentina 2017-08-29T14:33:21Z 2017-08-29T14:33:21Z 2016 info:eu-repo/semantics/article info:ar-repo/semantics/artículo info:eu-repo/semantics/publishedVersion http://hdl.handle.net/20.500.12123/1068 http://vetrecordopen.bmj.com/content/vetreco/3/1/e000162.full.pdf Maidana SS, et al. Cattle are a potential reservoir of bubaline herpesvirus 1 (BuHV1). Vet Rec Open 2016;3:e000162.doi:10.1136/vetreco-2015- 000162 2052-6113 (Online) http://dx.doi.org/10.1136/vetreco-2015-000162 eng info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) application/pdf Veterinary Record Open 3 (2016) |
| spellingShingle | Enfermedades de los Animales Ganado Bovino Herpesviridae Búfalo de Agua Animal Diseases Cattle Water Buffaloes Herpesvirus Maidana, Silvina Soledad Delgado, Fernando Oscar Vagnoni, Lucas Emilio Mauroy, Axel Thiry, Etienne Romera, Sonia Alejandra Cattle are a potential reservoir of bubaline herpesvirus 1 (BuHV1) |
| title | Cattle are a potential reservoir of bubaline herpesvirus 1 (BuHV1) |
| title_full | Cattle are a potential reservoir of bubaline herpesvirus 1 (BuHV1) |
| title_fullStr | Cattle are a potential reservoir of bubaline herpesvirus 1 (BuHV1) |
| title_full_unstemmed | Cattle are a potential reservoir of bubaline herpesvirus 1 (BuHV1) |
| title_short | Cattle are a potential reservoir of bubaline herpesvirus 1 (BuHV1) |
| title_sort | cattle are a potential reservoir of bubaline herpesvirus 1 buhv1 |
| topic | Enfermedades de los Animales Ganado Bovino Herpesviridae Búfalo de Agua Animal Diseases Cattle Water Buffaloes Herpesvirus |
| url | http://hdl.handle.net/20.500.12123/1068 http://vetrecordopen.bmj.com/content/vetreco/3/1/e000162.full.pdf http://dx.doi.org/10.1136/vetreco-2015-000162 |
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