Isolation and molecular cloning of genes from Myrciaria dubia “camu-camu” with potential use for biotechnological production of vitamin C
Myrciaria dubia “camu-camu” is a rich source of several bioactive phytochemicals and vitamin C (L-ascorbic acid, AsA). To gain insights about the genes involved in AsA biosynthesis in this plant species and consequently with potential use for its biotechnological production, here we report the isola...
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| Format: | info:eu-repo/semantics/article |
| Language: | Inglés |
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Society for Biology and Biotechnology
2018
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| Online Access: | https://repositorio.inia.gob.pe/handle/20.500.12955/737 |
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| author | Castro Gómez, Juan Carlos Cobos Ruiz, Marianela Maddox, J. Dylan Imán Correa, Sixto Alfredo Marapara del Aguila, Jorge Luis |
| author_browse | Castro Gómez, Juan Carlos Cobos Ruiz, Marianela Imán Correa, Sixto Alfredo Maddox, J. Dylan Marapara del Aguila, Jorge Luis |
| author_facet | Castro Gómez, Juan Carlos Cobos Ruiz, Marianela Maddox, J. Dylan Imán Correa, Sixto Alfredo Marapara del Aguila, Jorge Luis |
| author_sort | Castro Gómez, Juan Carlos |
| collection | Repositorio INIA |
| description | Myrciaria dubia “camu-camu” is a rich source of several bioactive phytochemicals and vitamin C (L-ascorbic acid, AsA). To gain insights about the genes involved in AsA biosynthesis in this plant species and consequently with potential use for its biotechnological production, here we report the isolation and molecular cloning of partial gene sequences of the D-mannose/L-galactose pathway. Degenerate primers designed by the multiple sequence alignment of related plant species were used to isolate in M. dubia the partial sequences of the six D-mannose/L-galactose pathway genes (GMP, GME, GGP, GPP, GDH and GLDH). The deduced protein sequences of the six genes have more than 81% sequence identity to rosids and asterids species, with a closer phylogenetic relationship to Eucalyptus grandis. In conclusion, gene sequences of the D-mannose/L-galactose pathway involved in AsA biosynthesis of M. dubia were successfully isolated and cloned and the phylogenetic analysis indicated that these genes have been relatively well conserved throughout of plant evolution, reflecting the importance of the enzymes of this metabolic pathway for plant growth and survival. Additionally, the isolation and cloning of these genes allow us to implement systems for biotechnological production of AsA. |
| format | info:eu-repo/semantics/article |
| id | INIA737 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Society for Biology and Biotechnology |
| publisherStr | Society for Biology and Biotechnology |
| record_format | dspace |
| spelling | INIA7372023-10-11T14:58:29Z Isolation and molecular cloning of genes from Myrciaria dubia “camu-camu” with potential use for biotechnological production of vitamin C Castro Gómez, Juan Carlos Cobos Ruiz, Marianela Maddox, J. Dylan Imán Correa, Sixto Alfredo Marapara del Aguila, Jorge Luis Clonación molecular Biotecnología molecular Filogenia molecular Biosíntesis del ácido L-ascórbico Frutas tropicales Camu-camu Gene cloning Molecular biotechnology Molecular phylogeny L-ascorbic acid biosynthesis Tropical fruit Recursos genéticos Myrciaria dubia “camu-camu” is a rich source of several bioactive phytochemicals and vitamin C (L-ascorbic acid, AsA). To gain insights about the genes involved in AsA biosynthesis in this plant species and consequently with potential use for its biotechnological production, here we report the isolation and molecular cloning of partial gene sequences of the D-mannose/L-galactose pathway. Degenerate primers designed by the multiple sequence alignment of related plant species were used to isolate in M. dubia the partial sequences of the six D-mannose/L-galactose pathway genes (GMP, GME, GGP, GPP, GDH and GLDH). The deduced protein sequences of the six genes have more than 81% sequence identity to rosids and asterids species, with a closer phylogenetic relationship to Eucalyptus grandis. In conclusion, gene sequences of the D-mannose/L-galactose pathway involved in AsA biosynthesis of M. dubia were successfully isolated and cloned and the phylogenetic analysis indicated that these genes have been relatively well conserved throughout of plant evolution, reflecting the importance of the enzymes of this metabolic pathway for plant growth and survival. Additionally, the isolation and cloning of these genes allow us to implement systems for biotechnological production of AsA. Peer reviewed 2018-11-13T14:34:25Z 2018-11-13T14:34:25Z 2016-11-03 info:eu-repo/semantics/article Castro, J.; Cobos, M.; Maddox, J.; Imán, S. A. & Marapara, J. L. (2016). Isolation and molecular cloning of genes from Myrciaria dubia “camu-camu” with potential use for biotechnological production of vitamin C. Plant Cell Biotechnology and Molecular Biology, 17(7-8), 415-427. Disponible en http://www.ikpress.org/index.php/PCBMB/article/view/1647 0972-2025 https://repositorio.inia.gob.pe/handle/20.500.12955/737 Plant Cell Biotechnology and Molecular Biology eng Plant Cell Biotechnology and Molecular Biology, vol.17, n.7-8, pp.415-427; 2016 http://www.ikpress.org/index.php/PCBMB/article/view/1647 http://www.ikpress.org/index.php/PCBMB/article/view/1647 info:eu-repo/semantics/restrictedAccess application/pdf application/pdf Perú Society for Biology and Biotechnology India Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Clonación molecular Biotecnología molecular Filogenia molecular Biosíntesis del ácido L-ascórbico Frutas tropicales Camu-camu Gene cloning Molecular biotechnology Molecular phylogeny L-ascorbic acid biosynthesis Tropical fruit Recursos genéticos Castro Gómez, Juan Carlos Cobos Ruiz, Marianela Maddox, J. Dylan Imán Correa, Sixto Alfredo Marapara del Aguila, Jorge Luis Isolation and molecular cloning of genes from Myrciaria dubia “camu-camu” with potential use for biotechnological production of vitamin C |
| title | Isolation and molecular cloning of genes from Myrciaria dubia “camu-camu” with potential use for biotechnological production of vitamin C |
| title_full | Isolation and molecular cloning of genes from Myrciaria dubia “camu-camu” with potential use for biotechnological production of vitamin C |
| title_fullStr | Isolation and molecular cloning of genes from Myrciaria dubia “camu-camu” with potential use for biotechnological production of vitamin C |
| title_full_unstemmed | Isolation and molecular cloning of genes from Myrciaria dubia “camu-camu” with potential use for biotechnological production of vitamin C |
| title_short | Isolation and molecular cloning of genes from Myrciaria dubia “camu-camu” with potential use for biotechnological production of vitamin C |
| title_sort | isolation and molecular cloning of genes from myrciaria dubia camu camu with potential use for biotechnological production of vitamin c |
| topic | Clonación molecular Biotecnología molecular Filogenia molecular Biosíntesis del ácido L-ascórbico Frutas tropicales Camu-camu Gene cloning Molecular biotechnology Molecular phylogeny L-ascorbic acid biosynthesis Tropical fruit Recursos genéticos |
| url | https://repositorio.inia.gob.pe/handle/20.500.12955/737 |
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