Optimized In vitro propagation and somatic embryogenesis of camu camu (Myrciaria dubia) cultivar Vitahuayo for enhanced commercial production
Background: Myrciaria dubia “camu-camu” a fruit-bearing shrub native to the Amazon, is known for its high vitamin C content. However, variations in vitamin C biosynthesis and accumulation among different cultivars present challenges for commercial production. This study aimed to establish an efficie...
| Autores principales: | , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Agricultural Research Communication Centre (ARCC Journals)
2025
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| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12955/2804 https://doi.org/10.18805/ag.DF-684 |
| _version_ | 1855490417937612800 |
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| author | Adrianzén, Pedro M. Pinedo Freyre, Sergio Fernando Valles, Barbara S. Marapara, Jorge L. Cobos, Marianela Rodríguez, Hicler N. Castro, Juan C. |
| author_browse | Adrianzén, Pedro M. Castro, Juan C. Cobos, Marianela Marapara, Jorge L. Pinedo Freyre, Sergio Fernando Rodríguez, Hicler N. Valles, Barbara S. |
| author_facet | Adrianzén, Pedro M. Pinedo Freyre, Sergio Fernando Valles, Barbara S. Marapara, Jorge L. Cobos, Marianela Rodríguez, Hicler N. Castro, Juan C. |
| author_sort | Adrianzén, Pedro M. |
| collection | Repositorio INIA |
| description | Background: Myrciaria dubia “camu-camu” a fruit-bearing shrub native to the Amazon, is known for its high vitamin C content. However, variations in vitamin C biosynthesis and accumulation among different cultivars present challenges for commercial production. This study aimed to establish an efficient in vitro regeneration protocol for the Vitahuayo cultivar through callus induction and somatic embryogenesis.
Methods: Stem and leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with 24-dichlorophenoxyacetic acid (24-D) and 6-benzylaminopurine (BAP) to induce callus formation. Somatic embryos were induced using Woody Plant Medium (WPM) supplemented with 2,4-D, thidiazuron (TDZ), indole-3-butyric acid (IBA), naphthaleneacetic acid (NAA) and kinetin (KIN).
Result: The best results for callogenesis were obtained with 1 mg/L of 24-D and 0.5 mg/L BAP. Somatic embryos were successfully induced with treatments T2 (2,4-D at 3 mg/L, NAA at 20 mg/L and KIN at 15 mg/L) showing 90% efficiency, while T3 (KIN at 0.3 mg/L and BAP at 0.05 mg/L) achieved 57.5% effectiveness. These findings offer great potential for the large-scale propagation of Vitahuayo, the optimization of its commercial production and the standardization of vitamin C biosynthesis. |
| format | Artículo |
| id | INIA2804 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Agricultural Research Communication Centre (ARCC Journals) |
| publisherStr | Agricultural Research Communication Centre (ARCC Journals) |
| record_format | dspace |
| spelling | INIA28042025-07-30T06:17:21Z Optimized In vitro propagation and somatic embryogenesis of camu camu (Myrciaria dubia) cultivar Vitahuayo for enhanced commercial production Adrianzén, Pedro M. Pinedo Freyre, Sergio Fernando Valles, Barbara S. Marapara, Jorge L. Cobos, Marianela Rodríguez, Hicler N. Castro, Juan C. ascorbic acid callus induction plant regeneration somatic embryogenesis ácido ascórbico inducción de callos regeneración vegetal embriogénesis somática https://purl.org/pe-repo/ocde/ford#4.04.00 cultivo de tejidos; tissue culture; biosíntesis; biosynthesis Background: Myrciaria dubia “camu-camu” a fruit-bearing shrub native to the Amazon, is known for its high vitamin C content. However, variations in vitamin C biosynthesis and accumulation among different cultivars present challenges for commercial production. This study aimed to establish an efficient in vitro regeneration protocol for the Vitahuayo cultivar through callus induction and somatic embryogenesis. Methods: Stem and leaf explants were cultured on Murashige and Skoog (MS) medium supplemented with 24-dichlorophenoxyacetic acid (24-D) and 6-benzylaminopurine (BAP) to induce callus formation. Somatic embryos were induced using Woody Plant Medium (WPM) supplemented with 2,4-D, thidiazuron (TDZ), indole-3-butyric acid (IBA), naphthaleneacetic acid (NAA) and kinetin (KIN). Result: The best results for callogenesis were obtained with 1 mg/L of 24-D and 0.5 mg/L BAP. Somatic embryos were successfully induced with treatments T2 (2,4-D at 3 mg/L, NAA at 20 mg/L and KIN at 15 mg/L) showing 90% efficiency, while T3 (KIN at 0.3 mg/L and BAP at 0.05 mg/L) achieved 57.5% effectiveness. These findings offer great potential for the large-scale propagation of Vitahuayo, the optimization of its commercial production and the standardization of vitamin C biosynthesis. 2025-07-30T06:17:21Z 2025-07-30T06:17:21Z 2025-03-12 info:eu-repo/semantics/article Adrianzén, P. M., Pinedo, S. F., Valles, B. S., Marapara, J. L., Cobos, M., Rodríguez, H. N., & Castro, J. C. (2025). Optimized in vitro propagation and somatic embryogenesis of camu camu (Myrciaria dubia) cultivar Vitahuayo for enhanced commercial production. Agricultural Science Digest, 45(3), 468–474. https://doi.org/10.18805/ag.DF-684 0253-150X http://hdl.handle.net/20.500.12955/2804 https://doi.org/10.18805/ag.DF-684 eng urn:issn:0253-150X Agricultural Research Communication Centre (ARCC Journals) info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ application/pdf application/pdf Agricultural Research Communication Centre (ARCC Journals) IN Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | ascorbic acid callus induction plant regeneration somatic embryogenesis ácido ascórbico inducción de callos regeneración vegetal embriogénesis somática https://purl.org/pe-repo/ocde/ford#4.04.00 cultivo de tejidos; tissue culture; biosíntesis; biosynthesis Adrianzén, Pedro M. Pinedo Freyre, Sergio Fernando Valles, Barbara S. Marapara, Jorge L. Cobos, Marianela Rodríguez, Hicler N. Castro, Juan C. Optimized In vitro propagation and somatic embryogenesis of camu camu (Myrciaria dubia) cultivar Vitahuayo for enhanced commercial production |
| title | Optimized In vitro propagation and somatic embryogenesis of camu camu (Myrciaria dubia) cultivar Vitahuayo for enhanced commercial production |
| title_full | Optimized In vitro propagation and somatic embryogenesis of camu camu (Myrciaria dubia) cultivar Vitahuayo for enhanced commercial production |
| title_fullStr | Optimized In vitro propagation and somatic embryogenesis of camu camu (Myrciaria dubia) cultivar Vitahuayo for enhanced commercial production |
| title_full_unstemmed | Optimized In vitro propagation and somatic embryogenesis of camu camu (Myrciaria dubia) cultivar Vitahuayo for enhanced commercial production |
| title_short | Optimized In vitro propagation and somatic embryogenesis of camu camu (Myrciaria dubia) cultivar Vitahuayo for enhanced commercial production |
| title_sort | optimized in vitro propagation and somatic embryogenesis of camu camu myrciaria dubia cultivar vitahuayo for enhanced commercial production |
| topic | ascorbic acid callus induction plant regeneration somatic embryogenesis ácido ascórbico inducción de callos regeneración vegetal embriogénesis somática https://purl.org/pe-repo/ocde/ford#4.04.00 cultivo de tejidos; tissue culture; biosíntesis; biosynthesis |
| url | http://hdl.handle.net/20.500.12955/2804 https://doi.org/10.18805/ag.DF-684 |
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