Matrix-assisted laser desorption ionization time-of-flight mass spectrometry combined with chemometrics for protein profiling and classification of boiled and extruded quinoa from conventional and organic crops
Quinoa is an Andean crop that stands out as a high-quality protein-rich and gluten-free food. However, its increasing popularity exposes quinoa products to the potential risk of adulteration with cheaper cereals. Consequently, there is a need for novel methodologies to accurately characterize the co...
| Main Authors: | , , , , |
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| Format: | Artículo |
| Language: | Inglés |
| Published: |
MDPI
2024
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| Subjects: | |
| Online Access: | https://hdl.handle.net/20.500.12955/2583 https://doi.org/10.3390/foods13121906 |
| _version_ | 1855490573867155456 |
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| author | Galindo Luján, Rocío Pont, Laura Quispe Jacobo, Fredy Enrique Sanz Nebot, Victoria Benavente, Fernando |
| author_browse | Benavente, Fernando Galindo Luján, Rocío Pont, Laura Quispe Jacobo, Fredy Enrique Sanz Nebot, Victoria |
| author_facet | Galindo Luján, Rocío Pont, Laura Quispe Jacobo, Fredy Enrique Sanz Nebot, Victoria Benavente, Fernando |
| author_sort | Galindo Luján, Rocío |
| collection | Repositorio INIA |
| description | Quinoa is an Andean crop that stands out as a high-quality protein-rich and gluten-free food. However, its increasing popularity exposes quinoa products to the potential risk of adulteration with cheaper cereals. Consequently, there is a need for novel methodologies to accurately characterize the composition of quinoa, which is influenced not only by the variety type but also by the farming and processing conditions. In this study, we present a rapid and straightforward method based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to generate global fingerprints of quinoa proteins from white quinoa varieties, which were cultivated under conventional and organic farming and processed through boiling and extrusion. The mass spectra of the different protein extracts were processed using the MALDIquant software (version 1.19.3), detecting 49 proteins (with 31 tentatively identified). Intensity values from these proteins were then considered protein fingerprints for multivariate data analysis. Our results revealed reliable partial least squares-discriminant analysis (PLS-DA) classification models for distinguishing between farming and processing conditions, and the detected proteins that were critical for differentiation. They confirm the effectiveness of tracing the agricultural origins and technological treatments of quinoa grains through protein fingerprinting by MALDI-TOF-MS and chemometrics. This untargeted approach offers promising applications in food control and the food-processing industry. |
| format | Artículo |
| id | INIA2583 |
| institution | Institucional Nacional de Innovación Agraria |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | INIA25832024-09-30T18:41:19Z Matrix-assisted laser desorption ionization time-of-flight mass spectrometry combined with chemometrics for protein profiling and classification of boiled and extruded quinoa from conventional and organic crops Galindo Luján, Rocío Pont, Laura Quispe Jacobo, Fredy Enrique Sanz Nebot, Victoria Benavente, Fernando Boiling Conventional Farming Extrusion Maldiquant MALDI-TOF-MS Multivariate Data Analysis Organic Farming Proteins Quinoa https://purl.org/pe-repo/ocde/ford#2.11.00 Boiling Ebullición Conventional farming Agricultura convencional Extrusion Spectrometry Espectrometría Multivariate analysis Análisis multivariante Data analysis Análisis de datos Organic agriculture Agricultura orgánica Quinoa Quinua Quinoa is an Andean crop that stands out as a high-quality protein-rich and gluten-free food. However, its increasing popularity exposes quinoa products to the potential risk of adulteration with cheaper cereals. Consequently, there is a need for novel methodologies to accurately characterize the composition of quinoa, which is influenced not only by the variety type but also by the farming and processing conditions. In this study, we present a rapid and straightforward method based on matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) to generate global fingerprints of quinoa proteins from white quinoa varieties, which were cultivated under conventional and organic farming and processed through boiling and extrusion. The mass spectra of the different protein extracts were processed using the MALDIquant software (version 1.19.3), detecting 49 proteins (with 31 tentatively identified). Intensity values from these proteins were then considered protein fingerprints for multivariate data analysis. Our results revealed reliable partial least squares-discriminant analysis (PLS-DA) classification models for distinguishing between farming and processing conditions, and the detected proteins that were critical for differentiation. They confirm the effectiveness of tracing the agricultural origins and technological treatments of quinoa grains through protein fingerprinting by MALDI-TOF-MS and chemometrics. This untargeted approach offers promising applications in food control and the food-processing industry. This study was supported by grant PID2021-127137OB-I00, unded byMCIN/AEI/10.13039/501100011033, and by “ERDF A way of making Europe”. The Bioanalysis group of the university of Barcelona is part of the INSA-UB Maria de Maeztu Unit of Excellence (Grant CEX2021-001234-M) funded by MCIN/AEI/FEDER, UE. 2024-09-30T18:41:18Z 2024-09-30T18:41:18Z 2024-06-17 info:eu-repo/semantics/article Galindo-Luján, R.; Pont, L.; Quispe-Jacobo, F.E.; Sanz-Nebot, V.; & Benavente, F. (2024). Matrix-assisted laser desorption ionization time-of-flight mass spectrometry combined with chemometrics for protein profiling and classification of boiled and extruded quinoa from conventional and organic crops. Foods,13(12),1906. doi:10.3390/foods13121906 2304-8158 https://hdl.handle.net/20.500.12955/2583 https://doi.org/10.3390/foods13121906 eng urn:issn:2304-8158 Foods info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ application/pdf application/pdf MDPI CH Instituto Nacional de Innovación Agraria Repositorio Institucional - INIA |
| spellingShingle | Boiling Conventional Farming Extrusion Maldiquant MALDI-TOF-MS Multivariate Data Analysis Organic Farming Proteins Quinoa https://purl.org/pe-repo/ocde/ford#2.11.00 Boiling Ebullición Conventional farming Agricultura convencional Extrusion Spectrometry Espectrometría Multivariate analysis Análisis multivariante Data analysis Análisis de datos Organic agriculture Agricultura orgánica Quinoa Quinua Galindo Luján, Rocío Pont, Laura Quispe Jacobo, Fredy Enrique Sanz Nebot, Victoria Benavente, Fernando Matrix-assisted laser desorption ionization time-of-flight mass spectrometry combined with chemometrics for protein profiling and classification of boiled and extruded quinoa from conventional and organic crops |
| title | Matrix-assisted laser desorption ionization time-of-flight mass spectrometry combined with chemometrics for protein profiling and classification of boiled and extruded quinoa from conventional and organic crops |
| title_full | Matrix-assisted laser desorption ionization time-of-flight mass spectrometry combined with chemometrics for protein profiling and classification of boiled and extruded quinoa from conventional and organic crops |
| title_fullStr | Matrix-assisted laser desorption ionization time-of-flight mass spectrometry combined with chemometrics for protein profiling and classification of boiled and extruded quinoa from conventional and organic crops |
| title_full_unstemmed | Matrix-assisted laser desorption ionization time-of-flight mass spectrometry combined with chemometrics for protein profiling and classification of boiled and extruded quinoa from conventional and organic crops |
| title_short | Matrix-assisted laser desorption ionization time-of-flight mass spectrometry combined with chemometrics for protein profiling and classification of boiled and extruded quinoa from conventional and organic crops |
| title_sort | matrix assisted laser desorption ionization time of flight mass spectrometry combined with chemometrics for protein profiling and classification of boiled and extruded quinoa from conventional and organic crops |
| topic | Boiling Conventional Farming Extrusion Maldiquant MALDI-TOF-MS Multivariate Data Analysis Organic Farming Proteins Quinoa https://purl.org/pe-repo/ocde/ford#2.11.00 Boiling Ebullición Conventional farming Agricultura convencional Extrusion Spectrometry Espectrometría Multivariate analysis Análisis multivariante Data analysis Análisis de datos Organic agriculture Agricultura orgánica Quinoa Quinua |
| url | https://hdl.handle.net/20.500.12955/2583 https://doi.org/10.3390/foods13121906 |
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