One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins
Seed proteins were extracted from the African yam bean (AYB; Sphenostylis stenocarpa), an underutilized West African food legume. One- and two-dimensional polyacrylamide gel electrophoresis was then used to analyze the albumin fraction, galactose-specific lectins purified on immobilized galactose−Se...
| Main Authors: | , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
American Chemical Society
2000
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/99870 |
| _version_ | 1855520076085592064 |
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| author | Machuka, J. Okeola, O. |
| author_browse | Machuka, J. Okeola, O. |
| author_facet | Machuka, J. Okeola, O. |
| author_sort | Machuka, J. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Seed proteins were extracted from the African yam bean (AYB; Sphenostylis stenocarpa), an underutilized West African food legume. One- and two-dimensional polyacrylamide gel electrophoresis was then used to analyze the albumin fraction, galactose-specific lectins purified on immobilized galactose−Sepharose 4B, and abundant non-lectin seed proteins left over following affinity chromatography. N-terminal sequencing of prominently resolved polypetide bands led to identification of proteins having sequence homology with characterized legume seed proteins, namely, mung bean seed albumin, pea α-fucosidase, soybean Kunitz-type trypsin inhibitor, an endochitinase, pea pathogenesis-related protein, and/or cowpea seed storage proteins. Minor lectin-like proteins lacking hemagglutinating activity against rabbit and human erythrocytes were also identified. Because proteins such as protease inhibitors, chitinases, pathogenesis-related proteins, and lectins are known to have antimetabolic effects, the findings from this study may have relevance in the acceptability, adoption, and utilization of AYB as human food. |
| format | Journal Article |
| id | CGSpace99870 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2000 |
| publishDateRange | 2000 |
| publishDateSort | 2000 |
| publisher | American Chemical Society |
| publisherStr | American Chemical Society |
| record_format | dspace |
| spelling | CGSpace998702024-08-27T10:35:21Z One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins Machuka, J. Okeola, O. legumes foods proteins pathogenesis Seed proteins were extracted from the African yam bean (AYB; Sphenostylis stenocarpa), an underutilized West African food legume. One- and two-dimensional polyacrylamide gel electrophoresis was then used to analyze the albumin fraction, galactose-specific lectins purified on immobilized galactose−Sepharose 4B, and abundant non-lectin seed proteins left over following affinity chromatography. N-terminal sequencing of prominently resolved polypetide bands led to identification of proteins having sequence homology with characterized legume seed proteins, namely, mung bean seed albumin, pea α-fucosidase, soybean Kunitz-type trypsin inhibitor, an endochitinase, pea pathogenesis-related protein, and/or cowpea seed storage proteins. Minor lectin-like proteins lacking hemagglutinating activity against rabbit and human erythrocytes were also identified. Because proteins such as protease inhibitors, chitinases, pathogenesis-related proteins, and lectins are known to have antimetabolic effects, the findings from this study may have relevance in the acceptability, adoption, and utilization of AYB as human food. 2000-06-01 2019-03-03T05:53:46Z 2019-03-03T05:53:46Z Journal Article https://hdl.handle.net/10568/99870 en Limited Access American Chemical Society Machuka, J. & Okeola, O. (2000). One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins. Journal of Agricultural and Food Chemistry, 48(6), 2296-2299. |
| spellingShingle | legumes foods proteins pathogenesis Machuka, J. Okeola, O. One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins |
| title | One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins |
| title_full | One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins |
| title_fullStr | One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins |
| title_full_unstemmed | One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins |
| title_short | One and two dimensional gel electrophoresic identification of African yam bean (Sphenostylis stenocarpa) seed proteins |
| title_sort | one and two dimensional gel electrophoresic identification of african yam bean sphenostylis stenocarpa seed proteins |
| topic | legumes foods proteins pathogenesis |
| url | https://hdl.handle.net/10568/99870 |
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