A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta)
The most commonly used method for measuring carotenoid concentration is high-performance liquid chromatography (HPLC). Nevertheless, easier, quicker, and less costly proxy methods exist. We aimed to determine the diagnostic performance of several proxy methods: the spectrophotometer, iCheck Carotene...
| Main Authors: | , , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
| Published: |
Public Library of Science
2018
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| Online Access: | https://hdl.handle.net/10568/99098 |
| _version_ | 1855538849274396672 |
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| author | Jaramillo Valencia, Angélica M. Londoño Hernandez, Luis Fernando Orozco, Juan Camilo Patiño, Gelver Belalcázar, John Eiver Davrieux, Fabrice Talsma, Elise F. |
| author_browse | Belalcázar, John Eiver Davrieux, Fabrice Jaramillo Valencia, Angélica M. Londoño Hernandez, Luis Fernando Orozco, Juan Camilo Patiño, Gelver Talsma, Elise F. |
| author_facet | Jaramillo Valencia, Angélica M. Londoño Hernandez, Luis Fernando Orozco, Juan Camilo Patiño, Gelver Belalcázar, John Eiver Davrieux, Fabrice Talsma, Elise F. |
| author_sort | Jaramillo Valencia, Angélica M. |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | The most commonly used method for measuring carotenoid concentration is high-performance liquid chromatography (HPLC). Nevertheless, easier, quicker, and less costly proxy methods exist. We aimed to determine the diagnostic performance of several proxy methods: the spectrophotometer, iCheck Carotene, and near-infrared spectroscopy using both a desktop (dNIRS) and a portable (pNIRS) device for the measurement of total carotenoid concentration (TCC) and all-trans-β-carotene concentration (trans-BC) in 30 fresh cassava (Manihot esculenta Crantz) storage roots in comparison with HPLC. The spectrophotometer presented the highest predictability for TCC, followed by iCheck, dNIRS, and pNIRS. The dNIRS showed the highest predictability and agreement for trans-BC. The pNIRS showed the poorest repeatability and greatest underestimations compared with HPLC. The agreement between all methods was lower for higher carotenoid concentration, with the exception of the spectrophotometer. According to our results, and for screening purposes, the measurement of carotenoids in fresh cassava roots can be carried out by spectrophotometer, iCheck Carotene and NIRS methods depending on the availability of equipment. |
| format | Journal Article |
| id | CGSpace99098 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Public Library of Science |
| publisherStr | Public Library of Science |
| record_format | dspace |
| spelling | CGSpace990982025-12-08T09:54:28Z A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta) Jaramillo Valencia, Angélica M. Londoño Hernandez, Luis Fernando Orozco, Juan Camilo Patiño, Gelver Belalcázar, John Eiver Davrieux, Fabrice Talsma, Elise F. biofotification manihot esculenta carotenoids cassava spectrophotometers equipment boiling plant breeding The most commonly used method for measuring carotenoid concentration is high-performance liquid chromatography (HPLC). Nevertheless, easier, quicker, and less costly proxy methods exist. We aimed to determine the diagnostic performance of several proxy methods: the spectrophotometer, iCheck Carotene, and near-infrared spectroscopy using both a desktop (dNIRS) and a portable (pNIRS) device for the measurement of total carotenoid concentration (TCC) and all-trans-β-carotene concentration (trans-BC) in 30 fresh cassava (Manihot esculenta Crantz) storage roots in comparison with HPLC. The spectrophotometer presented the highest predictability for TCC, followed by iCheck, dNIRS, and pNIRS. The dNIRS showed the highest predictability and agreement for trans-BC. The pNIRS showed the poorest repeatability and greatest underestimations compared with HPLC. The agreement between all methods was lower for higher carotenoid concentration, with the exception of the spectrophotometer. According to our results, and for screening purposes, the measurement of carotenoids in fresh cassava roots can be carried out by spectrophotometer, iCheck Carotene and NIRS methods depending on the availability of equipment. 2018-12-28 2019-01-17T13:57:15Z 2019-01-17T13:57:15Z Journal Article https://hdl.handle.net/10568/99098 en Open Access Public Library of Science Jaramillo, Angélica M.; Londoño, Luis Fernando; Orozco, Juan Camilo; Patiño, Gelver; Belalcazar, John; Davrieux, Fabrice & Talsma, Elise F. (2018). A comparison study of five different methods to measure carotenoids in biofortified yellow cassava ( Manihot esculenta ). Plos One, 13(12): e0209702. |
| spellingShingle | biofotification manihot esculenta carotenoids cassava spectrophotometers equipment boiling plant breeding Jaramillo Valencia, Angélica M. Londoño Hernandez, Luis Fernando Orozco, Juan Camilo Patiño, Gelver Belalcázar, John Eiver Davrieux, Fabrice Talsma, Elise F. A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta) |
| title | A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta) |
| title_full | A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta) |
| title_fullStr | A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta) |
| title_full_unstemmed | A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta) |
| title_short | A comparison study of five different methods to measure carotenoids in biofortified yellow cassava (Manihot esculenta) |
| title_sort | comparison study of five different methods to measure carotenoids in biofortified yellow cassava manihot esculenta |
| topic | biofotification manihot esculenta carotenoids cassava spectrophotometers equipment boiling plant breeding |
| url | https://hdl.handle.net/10568/99098 |
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