FecXBar a Novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine Sheep
Naturally occurring mutations in growth and differentiation factor 9 (GDF9) or bone morphogenetic protein 15 (BMP15) genes are associated with increased ovulation rate (OR) and litter size (LS) but also sterility. Observing the Tunisian Barbarine ewes of the "W" flock selected for improved prolifica...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | Inglés |
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Springer
2017
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10568/98486 |
| _version_ | 1855540119349493760 |
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| author | Lassoued, Narjess Ben Khlil, Zohra Woloszyn, Florent Rejeb, Ahmed Rekik, Mourad Fabre, Stephane |
| author_browse | Ben Khlil, Zohra Fabre, Stephane Lassoued, Narjess Rejeb, Ahmed Rekik, Mourad Woloszyn, Florent |
| author_facet | Lassoued, Narjess Ben Khlil, Zohra Woloszyn, Florent Rejeb, Ahmed Rekik, Mourad Fabre, Stephane |
| author_sort | Lassoued, Narjess |
| collection | Repository of Agricultural Research Outputs (CGSpace) |
| description | Naturally occurring mutations in growth and differentiation factor 9 (GDF9) or bone morphogenetic protein 15 (BMP15) genes are associated with increased ovulation rate (OR) and litter size (LS) but also sterility. Observing the Tunisian Barbarine ewes of the "W" flock selected for improved prolificacy, we found prolific and infertile ewes with streaky ovaries. Blood genomic DNA was extracted from a subset of low-ovulating, prolific and infertile ewes of the "W" flock, and the entire coding sequences of GDF9 and BMP15 were sequenced. We evidenced a novel polymorphism in the exon 1 of the BMP15 gene associated with increased prolificacy and sterility. This novel mutation called FecX Bar is a composite polymorphism associating a single nucleotide substitution (c.301G > T), a 3 bp deletion (c.302_304delCTA) and a C insertion (c.310insC) in the ovine BMP15 cDNA leading to a frame shift at protein position 101. Calculated in the "W" flock, the FecX Bar allele increased OR by 0.7 ova and LS by 0.3 lambs (p = 0.08). As for already identified mutations, homozygous females carrying FecX Bar exhibited streaky ovaries with a blockade at the primary stage of folliculogenesis as shown by histochemistry. Our investigation demonstrates a new mutation in the BMP15 gene providing a valuable genetic tool to control fecundity in Tunisian Barbarine, usable for diffusion program into conventional flocks looking for prolificacy improvement. |
| format | Journal Article |
| id | CGSpace98486 |
| institution | CGIAR Consortium |
| language | Inglés |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | CGSpace984862024-10-03T07:41:01Z FecXBar a Novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine Sheep Lassoued, Narjess Ben Khlil, Zohra Woloszyn, Florent Rejeb, Ahmed Rekik, Mourad Fabre, Stephane animal breeding genetics sheep small ruminants Naturally occurring mutations in growth and differentiation factor 9 (GDF9) or bone morphogenetic protein 15 (BMP15) genes are associated with increased ovulation rate (OR) and litter size (LS) but also sterility. Observing the Tunisian Barbarine ewes of the "W" flock selected for improved prolificacy, we found prolific and infertile ewes with streaky ovaries. Blood genomic DNA was extracted from a subset of low-ovulating, prolific and infertile ewes of the "W" flock, and the entire coding sequences of GDF9 and BMP15 were sequenced. We evidenced a novel polymorphism in the exon 1 of the BMP15 gene associated with increased prolificacy and sterility. This novel mutation called FecX Bar is a composite polymorphism associating a single nucleotide substitution (c.301G > T), a 3 bp deletion (c.302_304delCTA) and a C insertion (c.310insC) in the ovine BMP15 cDNA leading to a frame shift at protein position 101. Calculated in the "W" flock, the FecX Bar allele increased OR by 0.7 ova and LS by 0.3 lambs (p = 0.08). As for already identified mutations, homozygous females carrying FecX Bar exhibited streaky ovaries with a blockade at the primary stage of folliculogenesis as shown by histochemistry. Our investigation demonstrates a new mutation in the BMP15 gene providing a valuable genetic tool to control fecundity in Tunisian Barbarine, usable for diffusion program into conventional flocks looking for prolificacy improvement. 2017-12 2018-12-08T13:09:29Z 2018-12-08T13:09:29Z Journal Article https://hdl.handle.net/10568/98486 en Open Access Springer Lassoued, N., Benkhlil, Z., Woloszyn, F., Rejeb, A., Aouina, M., Rekik, M., Fabre, S. and Bedhiaf-Romdhani, S. 2017. FecXBar a Novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine Sheep. BMC Genetics 18:43. |
| spellingShingle | animal breeding genetics sheep small ruminants Lassoued, Narjess Ben Khlil, Zohra Woloszyn, Florent Rejeb, Ahmed Rekik, Mourad Fabre, Stephane FecXBar a Novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine Sheep |
| title | FecXBar a Novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine Sheep |
| title_full | FecXBar a Novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine Sheep |
| title_fullStr | FecXBar a Novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine Sheep |
| title_full_unstemmed | FecXBar a Novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine Sheep |
| title_short | FecXBar a Novel BMP15 mutation responsible for prolificacy and female sterility in Tunisian Barbarine Sheep |
| title_sort | fecxbar a novel bmp15 mutation responsible for prolificacy and female sterility in tunisian barbarine sheep |
| topic | animal breeding genetics sheep small ruminants |
| url | https://hdl.handle.net/10568/98486 |
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